Determination of mancozeb toxicity and biochemical effects in common carp (Cyprinus carpio)

Pooria Simakani, Mohammad Hadi Abolhasani, Seyyed Morteza Hoseini


The aim of this study was to investigate mancozeb toxicity and its effects on physiological characteristics of common carp. Fish were reared for one week to acclimatize with the experimental conditions. For 96h-LC50 determination, the fish were stocked in 16 aquaria at the density of 10 fish per aquarium. The aquaria were exposed to 8 mancozeb concentrations (two aquaria per concentration) for 96 h (0, 0.94, 1.87, 3.75, 7.50, 15, 30 and 60 mg/L). 96h-LC50 was calculated based on the fish mortality, being 8.03 (4.95-13.2) mg/L. For sub-acute test, the fish were exposed to 0 (control), 1.6, 2.4 and 3.2 mg/L mancozeb (20, 30 and 40% of the 96h-LC50) for one week. Blood samples were taken from each treatment for determination of plasma glucose, total protein, albumin, globulin, calcium, alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Mancozeb exposure led to increase in glucose and AST, and decrease in plasma proteins and ALT. In conclusion, mancozeb exposure causes stress response, health problem and tissue damage in common carp.


Carp, Mancozeb, Blood, Toxicity, Pesticide.

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