Pectinase enzyme from Streptomyces coelicoflavus GIAL86 isolated from Meyghan Salt Lake, Arak, Iran

Ensieh Salehghamari, Zohre Nasrollahzadeh, Mohammad Tahmaseb, Mohammad Ali Amoozegar

Abstract

Aquatic saline ecosystems are suitable environments for isolation of microorganisms with high diversity and widely used biotechnology features. The pectinase enzyme is one of the most important commercial enzymes with high potential in food and pharmaceutical industries. Therefore, the discovery of microorganisms with new characteristics has always been a focus of research. As such, a pectinase producing actinomycete Streptomyces coelicoflavus GIAL86 was isolated from Meyghan Salt Lake of Arak located in Markazi Province of Iran. This strain was screened among 35 isolates of halotolerant actinomycetes with the highest production of pectinase enzymes. It was also found that production of pectate lyase, pectin esterase and polygalacturonase increased simultaneously with the logarithmic growth of the strain and its maximum production is at the time of stationary phase beginning. Also, some actinomycete strains with more pectinase activity were identified by molecular identification and their phylogenic relationships were investigated.

Keywords

Actinomycete, Halotolerant, Pectin methyl esterase, Pectate lyase, Poly galactoronase.

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References

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