Effect of copper on the characterization of proteins in the Spiny lobster, Panulirus homarus homarus (Linnaeus,1758)

Maharajan Athisuyambulingam, Sundar Rajalakshmi, Manambrakat Vijayakumara

Abstract

Copper is most toxic metal in marine organisms. Characterization of protein occurring in the metabolically active tissues of muscle (MU), hepatopancreas (HP) and gills (GL) of the spiny lobster, Panulirus homarus homarus on exposure to two sub-lethal doses (9.55 and 19.1 µg/l) of copper were studied for 28 days of exposure (DoE). The electrophoretic pattern of muscle, hepatopancreas and gill proteins revealed 12, 8 and 8 slow moving bands (control). The number of bands decreased to 8 and 7, 6 and 5, 6 and 4 after 7 days of exposure to 9.55 µg/l and 19.1 µg/l concentrations of copper, respectively. After 28 days, the protein bands decreased to 7 and 6, 5 and 4, 4 and 4 at 9.55 µg/l and 19.1 µg/l concentrations of copper, respectively. Present study to indicate that to avoid the Cupro-Nickel coil in lobster holding centers in chiller plants used for cooling of water was found to be responsible for the mortality of lobsters during live transportation.

Keywords

Panulirus homarus homarus, Copper, SDS PAGE, Protein

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