Evaluation of toxicity and biochemical effects of the Oxadiargyl in Common carp (Cyprinus carpio L.)

Hossein Emadi, Somayeh Shariatzadeh, Shahla Jamili, Ali Mashinchian


This study aimed to investigate the effects of sublethal concentrations of oxadiargyl herbicide on growth performance, serum biochemical parameters and liver histology of the common carp, Cyprinus carpio, during 30 days exposure period. Carp fingerlings were randomly divided into four duplicate groups. Experimental groups were exposed to the concentration of 0.1, 0.3 and 0.5 ppm of oxadiargyl, while the control group was kept in toxicant free. During the experiment, none of the control fish died. Weight gain and condition factor decreased, while food conversion ratio increased in fish exposed to 0.5 ppm of oxadiargyl. Exposure to oxadiargyl significantly increased serum ALT, AST and ALP, as well as glucose levels, while cholesterol, total protein and albumin were significantly decreased in the exposed fish groups depend on oxadiargyl concentrations and exposure time. Diffuse and focal necrosis mainly as perivascular necrosis fibrosis, increase in size and number of melanomacrophage centers, bile duct hyperplasia, hyperemia and hemorrhage, fatty changes in the hepatocytes, fibrocyte aggregation and focal inflammatory cells were also the histological le­sions observed in the liver of exposed fish. These results showed that oxadiargyl is highly toxic to common carp and had negative effects on the growth performance, serum biochemical parameters and the liver tissue of common carp.


Carp, Growth performance, Biochemical parameters, Histopathology, Liver.

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Abdul-Farah M., Ateeq B., Ali M.N., Ahmad W. (2004). Studies on lethal concentrations and toxicity stress of some xenobiotics on aquatic organisms. Chemosphere, 55: 257-265.

Ahmadivand S., Farahmand H., Mirvaghefi A., Eagderi S., Shokrpoor S., Rahmati-Holasoo H. (2014). Histopathological and haematological response of male rainbow trout (Oncorhynchus mykiss) subjected to butachlor. Veterinarni Medicina, 59: 433-439.

Ahmadivand S., Farahmand H., Mirvaghefi A., Eagderi S., Zargar A. (2015). Effects of (anti) androgenic endocrine disruptors (DEHP and Butachlor) on immunoglobulin M (IgM) and leukocytes counts of male rainbow trout (Oncorhynchus mykiss). Bulletin of Environmental Contamination and Toxicology, 94: 695-700.

Ahmadivand S., Farahmand H., Teimoori-Toolabi L., Mirvaghefi A., Eagderi S., Geerinckx T., Shokrpoor S., Rahmati-Holasoo H. (2016). Boule gene expression underpins the meiotic arrest in spermatogenesis in male rainbow trout (Oncorhynchus mykiss) exposed to DEHP and butachlor. General and Comparative Endocrinology, 225: 235-241.

Ahmed S., Singh-Chauhan B. (2015). Efficacy and phytotoxicity of different rates of oxadiargyl and pendimethalin in dry-seeded rice (Oryza sativa L.) in Bangladesh. Crop Protection, 72: 169-174.

Ajani F., Oluyinka-Ajiboye A., Oluwatosin-Oyelowo O. (2015). Effects of Oxadiazon on Nutrient Utilization and Growth of African Catfish (Clarias gariepinus). American Journal of Agricultural Science, 2: 121-125.

Bálint T., Ferenczy J., Kátai F., Kiss I., Kráczer L., Kufcsák O., Láng G., Polyhos C., Szabó I., Szegletes T., Nemcsók J. (1997). Similarities and differences between the massive eel (Anguilla anguilla L) devastations that occurred in Lake Balaton in 1991 and 1995. Ecotoxicology and Environmental Safety, 37: 17-23.

Barton B.A. (2002). Stress in fishes: a diversity of responses with particular reference to changes in circulating corticosteroids. Integrative and Comparative Biology, 42: 517-25.

Blahova J., Modra H., Sevcikova M., Marsalek P., Zelnickova L., Skoric M., Svobodova Z. (2014). Evaluation of biochemical, haematological, and histopathological responses and recovery ability of common carp (Cyprinus carpio L.) after acute exposure to atrazine herbicide. BioMed Research International, 98094: 1-8.

Eagderi S., Mojazi Amiri B., Adriaens D. (2013). Description of the ovarian follicle maturation of the migratory adult female bulatmai barbel (Luciobarbus capito, Güldenstädt 1772) in captivity. Iranian Journal of Fisheries Sciences, 12(3): 550-560.

Gokcimen A., Gulle K., Demirin H., Bayram D., Kocak A., Altuntas I. (2007). Effects of diazinon at different doses on rat liver and pancreas tissues. Pesticide Biochemistry and Physiology, 87: 103-108.

Grung M., Lin Y., Zhang H., Steen A.O., Huang J., Zhang G., Larssen T. (2015). Pesticide levels and environmental risk in aquatic environments in China: A review. Environment International, 81: 87-97

Hewitson T.D., Wigg B., Becker G.J. (2010). Tissue preparation for histochemistry: fixation, embedding and antigen retrieval for light microscopy. In: T.D., Hewitson, I.A. Darby (Eds.), Histology Protocols. Humana Press, New York. pp: 3-18.

Hwang I. T., Hong K.S., Choi J.S., Kim H.R., Jeon D.J., Cho K.Y. (2004). Protoporphyrinogen ix-oxidizing activities involved in the mode of action of a new compound N-[4-Chloro-2-Fluoro-5-{3-(2-Fluorophen-yl)-5-Methyl-4,5-Dihydroisoxazol-5-Yl-Methoxy}-Ph-enyl]-3,4,5,6-Tetrahydrophthalimide. Pesticide Bioche-mistry and Physiology, 80: 123-130.

Kane A.M., Soltani M, Ebrahimzahe-Mousavi H.A, Pakzad K. (2016). Influence of probiotic, Lactobacillus plantarum on serum biochemical and immune parameters in vaccinated rainbow trout (Oncorhynchus mykiss) against streptococcosis/lactococosis. International Journal of Aquatic Biology, 4: 285-294.

Mahmoudi M., Rahnemaie R., Eshaghi A., Malakouti M.J. (2013). Kinetics of degradation and adsorption-desorption isotherms of thiobencarb and oxadiargyl in calcareous paddy fields. Chemosphere, 91: 1009-1017.

Monjezi N., Razmjo J., Karimmojeni H. (2015). Valerian (Valeriana officinalis L.) tolerance to some post-emergence herbicides. Journal of Plant Protection Research, 55: 415-420.

Nayak A.K., Das B.K., Kohli M.P.S., Mukherjee S.C. (2004). The immunosuppressive effect of alpha-permethrin on Indian major carp, rohu (Labeo rohita Ham.). Fish and Shellfish Immunology, 16: 41-50.

Nethra N.S., Jagannath S. (2011). Phytotoxic effect of oxadiargyl on germination and early growth of sunflower (Helianthus annuus L.) and maize (Zea mays L.). Archives of Phytopathology and Plant Protection, 44: 1901-1907.

Poleksic V., Karan V. (1999). Effects of trifluralin on carp: biochemical and histological evaluation. Ecotoxicology and Environmental Safety, 43: 213-221.

Ribeiro H.J., Procópio M.S., Gomes J.M.M., Vieira F.O., Russo R.C., Balzuweit K., Chiarini-Garcia H., Santana-Castro A.C., Rizzo E., Corrêa-Jr J.D. (2011). Functional dissimilarity of melanomacrophage centres in the liver and spleen from females of the teleost fish Prochilodus argenteus. Cell Tissue Research, 346: 417-425.

Richert L., Price S., Chesne C., Maita K., Carmichael N. (1996). Comparison of the induction of hepatic peroxisome proliferation by the herbicide oxadiazon in vivo in rats, mice, and dogs and in vitro in rat and human hepatocytes. Toxicology and Applied Pharmacology, 141: 35-43.

Saravanan M., Kim J.Y., Hur K.J., Ramesh M., Hur J-H. (2017). Responses of the freshwater fish Cyprinus carpio exposed to different concentrations of butachlor and oxadiazon. Biocatalysis and Agricultural Biotechnology, 11: 275-281.

Steinberg C.E.W., Lorenz R., Spieser O.H. (1995). Effects of atrazine on swimming behavior of zebrafish, Brachydanio rerio. Water Research, 29: 981-985.

Sweilum M.A. (2006). Effect of sublethal toxicity of some pesticides on growth parameters, haematological properties and total production of Nile tilapia (Oreochromis niloticus L.) and water quality of ponds. Aquaculture Research, 37: 1079-1089.

Wolf J.C., Wheeler J.R. (2018). A critical review of histopathological findings associated with endocrine and non-endocrine hepatic toxicity in fish models. Aquatic Toxicology, 197: 60-78.

Xing H., Liu T., Zhang Z., Wang X., Xu S. (2015). Acute and subchronic toxic effects of atrazine and chlorpyrifos on common carp (Cyprinus carpio L.): Immunotoxicity assessments. Fish and Shellfish Immunology, 45: 327-333.

Zanjani S.A., Emadi H., Jamili S., Mashinchian A. (2017). DNA damage and hematological changes in Common carp (Cyprinus carpio) exposed to oxadiazon. International Journal of Aquatic Biology, 5: 387-392.


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