Evaluation of oxidative stress induced by cadmium and comparative antioxidant effects of Shirazi thyme (Zataria multiï¬‚ora Boiss) and vitamin E in common carp (Cyprinus carpio)

Mohammad Mohiseni; Dara Bagheri; Mahdi Banaee; Behzad Nematdust Haghi

doi:10.22034/ijab.v5i6.318

Authors

Mohammad Mohiseni
[email protected]
Department of Fisheries, Natural Resources and Environmental Faculty, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.
Dara Bagheri Department of Fisheries, Natural Resources and Environmental Faculty, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.
Mahdi Banaee Department of Fisheries, Natural Resources and Environmental Faculty, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.
Behzad Nematdust Haghi Department of Fisheries, Natural Resources and Environmental Faculty, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.

Vol. 5 No. 6 (2017): December

Articles

December 12, 2017

Downloads

PDF

Abstract
How to Cite
Metrics
References

Shirazi thyme is an active phytobiotc contains phenolic compounds and flavonoids which have strong antioxidant properties. This study was conducted to investigate the potential protective effects of Shirazi thyme compared to that of vitamin E against cadmium toxicity. Common carp juveniles (34±3 g) were divided into four groups and fed by three different diets including commercial diet without any additive (for control and metal only group) and supplemented with either 1% ground Shirazi thyme or 100 mg/kg vitamin E. All treatments except the control were exposed to sublethal concentration of waterborne cadmium (1.5 mg/L free ion) for 15 d and liver, kidney and gill were sampled 3, 7, 10 and 15 days after the metal exposure. Results showed that treatment of the fish with cadmium for 15 days resulted in a significant reduction in glutathione reductase (GR), glutathione-S-transferase (GST) and catalase (CAT) and led to liver, kidney and gills dysfunction. On the other hand, the level of malondialdehyde (MDA) significantly increased during metal exposure. Supplementation of diets with Shirazi thyme and vitamin E led to a significant protection against metal exposure in different tissues. Moreover, Shirazi thyme was found to be as effective as vitamin E. The current finding can provide a useful reference for stress protective effects of thyme and its beneficial role in aquaculture.

Mohiseni, M., Bagheri, D., Banaee, M., & Nematdust Haghi, B. (2017). Evaluation of oxidative stress induced by cadmium and comparative antioxidant effects of Shirazi thyme (Zataria multiï¬‚ora Boiss) and vitamin E in common carp (Cyprinus carpio). International Journal of Aquatic Biology, 5(6), 360–369. https://doi.org/10.22034/ijab.v5i6.318

Download Citation

Aebi H. (1984). Catalase in vitro. Methods in enzymology, 105: 121-126.

Alí§ií§ek Z. (2011). The effects of thyme (Thymus vulgaris L.) oil concentration on liquid-smoked vacuum-packed rainbow trout (Oncorhynchus mykiss Walbaum, 1792) fillets during chilled storage. Food chemistry, 128(3): 683-688.

Almeida J., Diniz Y., Marques S., Faine L., Ribas B., Burneiko R., Novelli E. (2002). The use of the oxidative stress responses as biomarkers in Nile tilapia (Oreochromis niloticus) exposed to in vivo cadmium contamination. Environment International, 27(8): 673-679.

Antache A., Cristea V., Grecu I., CreÅ£u M. (2014). The synergistic influence of thymus vulgaris and vitamin E on growth performance and oxidative stress at Oreochromis niloticus species. Seria Zootehnie, 62: 85-90.

Chaurasia S.S., Kar A. (1997). Protective effects of vitamin E against lead-induced deterioration of membrane associated type-I iodothyronine 5"²-monodeiodinase (5"² DI) activity in male mice. Toxicology, 124(3): 203-209.

Chow C.K. (1991). Vitamin E and oxidative stress. Free Radical Biology and Medicine, 11(2): 215-232.

Cinar M., Yigit A., Eraslan G. (2010). Effects of vitamin C or vitamin E supplementation on cadmium induced oxidative stress and anaemia in broilers. Revue de Médecine Vétérinaire, 161(10): 449-454.

Cohen M.B., Duvel D.L. (1988). Characterization of the inhibition of glutathione reductase and the recovery of enzyme activity in exponentially growing murine leukemia (11210) cells treated with 1, 3-bis (2-chloroethyl) 1-nitrosourea. Biochemical Pharmacology, 37(17): 3317-3320.

Dorojan O.G., Placinta S., Petrea S. (2014). The influence of some phytobiotics (thyme, seabuckthorn) on growth performance of stellate sturgeon (A. stellatus, Pallas, 1771) in an industrial recirculating aquaculture system. Scientific Papers Animal Science and Biotechnologies, 47(1): 205-210.

Ehsani A., Jasour M.S., Hashemi M., Mehryar L., Khodayari M. (2014). Zataria multiflora Boiss essential oil and sodium acetate: how they affect shelf life of vacuum"packaged trout burgers. International Journal of Food Science and Technology, 49(4): 1055-1062.

El-Demerdash F.M., Yousef M.I., Kedwany F.S., Baghdadi H.H. (2004). Cadmium-induced changes in lipid peroxidation, blood hematology, biochemical parameters and semen quality of male rats: protective role of vitamin E and Î²-carotene. Food and chemical Toxicology, 42(10): 1563-1571.

El-Nekeety A.A., Mohamed S.R., Hathout A.S., Hassan N. S., Aly S.E., Abdel-Wahhab M.A. (2011). Antioxidant properties of Thymus vulgaris oil against aflatoxin-induce oxidative stress in male rats. Toxicon, 57(7): 984-991.

Fariss M.W. (1991). Cadmium toxicity: unique cytoprotective properties of alpha tocopheryl succinate in hepatocytes. Toxicology, 69(1): 63-77.

Farombi E., Adelowo O., Ajimoko Y. (2007). Biomarkers of oxidative stress and heavy metal levels as indicators of environmental pollution in African cat fish (Clarias gariepinus) from Nigeria Ogun River. International Journal of Environmental Research and Public Health, 4(2): 158-165.

Gate L., Paul J., Ba G.N., Tew K., Tapiero H. (1999). Oxidative stress induced in pathologies: the role of antioxidants. Biomedicine and Pharmacotherapy, 53(4): 169-180.

Gupta R.S., Gupta E.S., Dhakal B.K., Thakur A.R., Ahnn J. (2004). Vitamin C and vitamin Ð• protect the rat testes from cadmium-induced reactive oxygen species. Molecules and Cells, 17(1): 132-139.

Habig W.H., Pabst M.J., Jakoby W.B. (1974). Glutathione S-transferases the first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249(22): 7130-7139.

Hamzawy M.A., El-Denshary E., Hassan N.S., Mannaa F., Abdel-Wahhab M.A. (2012). Antioxidant and hepatorenoprotective effect of thyme vulgaris extract in rats during aflatoxicosis. Global Journal Pharmacology, 6(2): 106-117.

Harabawy A.S., Mosleh Y.Y. (2014). The role of vitamins A, C, E and selenium as antioxidants against genotoxicity and cytotoxicity of cadmium, copper, lead and zinc on erythrocytes of Nile tilapia, Oreochromis niloticus. Ecotoxicology and Environmental Safety, 104: 28-35.

Hassan A., Barakat I. (2008). Assessment of oxidative stress induced by nickel chloride and antioxidant effects of Basil (Ocimum basilicum L.) and Thyme (Tymus vulgaris L.). Journal of Genetic Engineering and Biotechnology, 6: 29-38.

Hosseini S.A., Meimandipour A., Alami F., Mahdavi A., Mohiti-Asli M., Lotfollahian H., Cross D. (2013). Effects of ground thyme and probiotic supplements in diets on broiler performance, blood biochemistry and immunological response to sheep red blood cells. Italian Journal of Animal Science, 12(1): 116-120.

Hosseinzadeh H., Ramezani M., Salmani G.-A. (2000). Antinociceptive, anti-inflammatory and acute toxicity effects of Zataria multiflora Boiss extracts in mice and rats. Journal of Ethnopharmacology, 73(3): 379-385.

Jurczuk M., Brzóska M.M., Moniuszko-Jakoniuk J., GaÅ‚aÅ¼yn-Sidorczuk M., Kulikowska-KarpiÅ„ska E. (2004). Antioxidant enzymes activity and lipid peroxidation in liver and kidney of rats exposed to cadmium and ethanol. Food and Chemical Toxicology, 42(3): 429-438.

Kalay M., Ay í–., Canli M. (1999). Heavy metal concentrations in fish tissues from the Northeast Mediterranean Sea. Bulletin of Environmental Contamination and Toxicology, 63(5): 673-681.

Kandaswami C., Middleton E. (1994). Free Radicals in Diagnostic Medicine. In: D. Armstrong (Ed.). Free Radicals in Diagnostic Medicine. New York: Springer pp: 351-376.

Kaushik S. (1995). Nutrient requirements, supply and utilization in the context of carp culture. Aquaculture, 129(1): 225-241.

Kavoosi G., Teixeira da Silva J.A., Saharkhiz M. J. (2012). Inhibitory effects of Zataria multiflora essential oil and its main components on nitric oxide and hydrogen peroxide production in lipopolysaccharide"stimulated macrophages. Journal of Pharmacy and Pharmacology, 64(10): 1491-1500.

Kruger N.J. (1994). The Bradford method for protein quantitation. Basic protein and peptide protocols: 9-15.

McCay P.B. (1985). Vitamin E: interactions with free radicals and ascorbate. Annual review of nutrition, 5(1): 323-340.

Mekkawy I.A., Mahmoud U.M., Wassif E.T., Naguib M. (2011). Effects of cadmium on some haematological and biochemical characteristics of Oreochromis niloticus (Linnaeus, 1758) dietary supplemented with tomato paste and vitamin E. Fish physiology and biochemistry, 37(1): 71-84.

Miura K., Kikuzaki H., Nakatani N. (2002). Antioxidant activity of chemical components from sage (Salvia officinalis L.) and thyme (Thymus vulgaris L.) measured by the oil stability index method. Journal of Agricultural and Food chemistry, 50(7): 1845-1851.

Mohiseni M., Asayesh S., Shafiee Bazarnoie S., Mohseni F., Moradi N., Matouri M., Mirzaee N. (2016). Biochemical alteration induced by cadmium and lead in common carp via an experimental food chain. Iranian Journal of Toxicology, 10(4): 25-32.

Mohiseni M., Farhangi M., Agh N., Mirvaghefi A., Talebi K. (2017a). Toxicity and Bioconcentration of Cadmium and Copper in Artemia Urmiana Nauplii. Iranian Journal of Toxicology, 11(1): 33-41.

Mohiseni M., Sepidnameh M., Bagheri, D., Banaee, M., Nematdust Haghi, B. (2017b). Comparative effects of Shirazi thyme and vitamin E on some growth and plasma biochemical changes in common carp (Cyprinus carpio) during cadmium exposure. Aquaculture Research, 48(9): 4811-4821.

Montero D., Marrero M., Izquierdo M., Robaina L., Vergara J., Tort L. (1999). Effect of vitamin E and C dietary supplementation on some immune parameters of gilthead seabream (Sparus aurata) juveniles subjected to crowding stress. Aquaculture, 171(3): 269-278.

Ochi T., Takahashi K., Ohsawa M. (1987). Indirect evidence for the induction of a prooxidant state by cadmium chloride in cultured mammalian cells and a possible mechanism for the induction. Mutation Research, 180(2): 257-266.

Oliveira M., Ahmad I., Maria V., Pacheco M., Santos M. (2010). Antioxidant responses versus DNA damage and lipid peroxidation in golden grey mullet liver: a field study at Ria de Aveiro (Portugal). Archives of Environmental Contamination and Toxicology, 59(3): 454-463.

Ortuño J., Cuesta A., Esteban M.A., Meseguer J. (2001). Effect of oral administration of high vitamin C and E dosages on the gilthead seabream (Sparus aurata L.) innate immune system. Veterinary Immunology and Immunopathology, 79(3): 167-180.

Rice-Evans C., Miller N., Paganga G. (1997). Antioxidant properties of phenolic compounds. Trends in Plant Science, 2(4): 152-159.

Ringwood A.H., Hoguet J., Keppler C., Gielazyn M., Ward B., Rourk A. (2003). Cellular biomarkers (lysosomal destabilization, glutathione & lipid peroxidation) in three common estuarine species: a methods handbook. South Carolina: Marine Resources Research Institute, South Carolina Department of Natural Resources Charleston.

Romeo M., Bennani N., Gnassia-Barelli M., Lafaurie M., Girard J. (2000). Cadmium and copper display different responses towards oxidative stress in the kidney of the sea bass Dicentrarchus labrax. Aquatic Toxicology, 48(2): 185-194.

Ruangsomboon S., Wongrat L. (2006). Bioaccumulation of cadmium in an experimental aquatic food chain involving phytoplankton (Chlorella vulgaris), zooplankton (Moina macrocopa), and the predatory catfish Clarias macrocephalusí— C. gariepinus. Aquatic Toxicology, 78(1): 15-20.

Sadek K., Ahmed H., Ayoub M., Elsabagh M. (2014). Evaluation of Digestarom and thyme as phytogenic feed additives for broiler chickens. European Poultry Science, 78: 1-12.

Sajed, H., Sahebkar, A., Iranshahi, M. (2013). Zataria multiflora Boiss (Shirazi thyme)"”an ancient condiment with modern pharmaceutical uses. Journal of Ethnopharmacology, 145(3): 686-698.

Sayeed I., Parvez S., Pandey S., Bin-Hafeez B., Haque R., Raisuddin S. (2003). Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, Channa punctatus Bloch. Ecotoxicology and Environmental Safety, 56(2): 295-301.

Sengí¼l T., Yurtseven S., Cetin M., Kocyigit A., Sögí¼t B. (2008). Effect of thyme (T. vulgaris) extracts on fattening performance, some blood parameters, oxidative stress and DNA damage in Japanese quails. Journal of Animal and Feed Sciences, 17(4): 608-620.

Sevcikova M., Modra H., Slaninova A., Svobodova Z. (2011). Metals as a cause of oxidative stress in fish: a review. Vet Med, 56(11): 537-546.

Soleimany V., Banaee M., Mohiseni M., Nematdoost Hagi B., Mousavi Dehmourdi L. (2016). Evaluation of pre-clinical safety and toxicology of Althaea officinalis extracts as naturopathic medicine for common carp (Cyprinus carpio). Iranian Journal of Fisheries Sciences, 15(2): 613-629.

Sönmez A.Y., Bilen S., Alak G., Hisar O., YanÄ±k T., Biswas, G. (2015). Growth performance and antioxidant enzyme activities in rainbow trout (Oncorhynchus mykiss) juveniles fed diets supplemented with sage, mint and thyme oils. Fish physiology and biochemistry, 41(1): 165-175.

Stohs S., Bagchi D. (1995). Oxidative mechanisms in the toxicity of metal ions. Free Radical Biology and Medicine, 18(2): 321-336.

Tan X.Y., Luo Z., Zhang G.Y., Liu X.J., Jiang M. (2010). Effect of dietary cadmium level on the growth, body composition and several hepatic enzymatic activities of juvenile yellow catfish, Pelteobagrus fulvidraco. Aquaculture Research, 41(7): 1022-1029.

íœndeÄŸer íœ., BaÅŸaran A., Degen G., BaÅŸaran N. (2009). Antioxidant activities of major thyme ingredients and lack of (oxidative) DNA damage in V79 Chinese hamster lung fibroblast cells at low levels of carvacrol and thymol. Food and Chemical Toxicology, 47(8): 2037-2043.

Vinodhini R., Narayanan M. (2009). The impact of toxic heavy metals on the hematological parameters in common carp (Cyprinus carpio L.). Iranian Journal of Environmental Health, Science and Engineering, 6(1): 23-28.

YÄ±lmaz S., Ergí¼n S., í‡elik E.Åž. (2012). Effects of herbal supplements on growth performance of sea bass (Dicentrarchus labrax): Change in body composition and some blood parameters. Journal of BioScience and Biotechnology, 1(3): 217-222.

YÄ±lmaz S., Ergí¼n S., í‡elik E.Åž. (2013). Effect of dietary herbal supplements on some physiological conditions of sea bass Dicentrarchus labrax. Journal of Aquatic Animal Health, 25(2): 98-103.