Caspian Sea’s Navicula salinicola Hustedt 1939 and effect of the prolonged culture on its fatty acid profile

Ehsan Etesami, Farkhondeh Saba, Mostafa Noroozi, Mohammad Ali Amoozegar, Gholamreza Bakhshi Khaniki, Seyed Abolhassan Shahzadeh Fazeli


Diatoms are a potent source of polyunsaturated fatty acids. This study was conducted for screening a Naviculoid diatom strains from the southern Caspian Sea with analyzing its lipid production and accumulation potentials. The isolate was identified as Navicula salinicola strain IBRC-M 5083 based on micro-morphological characterization and analysis of 18S rRNA genomic region. Navicula salinicola were cultured in the f/2 medium under both normal and prolonged culture (21 days) conditions. Total lipid percentages of this strain were found to be 31.83% under normal condition and 43.72±1.4% in prolonged culture respectively on the basis of their dry cell weight (DCW). Also, the oil droplets were detected in 21 days’ cells as shown by Sudan Black B staining experiments. Furthermore, the main fatty acids were found by Gas Chromatography analyses of this strain under prolonged condition to be Eicosapentaenoic acid (25.58%TFA). Such oil accumulation capabilities seem to be promising for performing further studies on this strain as a source of Omega-3 in aquafeed, pharmaceutical and biofuel industries.


Algae, Omega3, Eicosapentaenoic acid, Docosahexaenoic acid.

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