Preliminary investigation on the tail regeneration of the juvenile marine polychaete, Perinereis wilsoni
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In Perinereis wilsoni, gonad formation is thought to depend on germ cells supplied by the tail-end segment (pygidium). In this study, we investigated the regenerative capacity of germ cells in the pygidium following posterior amputation, using vasa as a putative germ cell marker. At 3 days post-amputation (dpa), pw-vasa-positive cells were scattered around the amputated area of the posterior segment, with some located near germ cell clusters in the distal parapodial region of the adjacent unamputated segment. By 4 dpa, pw-vasa expression was observed in the segment addition zone. At 7 dpa, pw-vasa was detected in germ cell clusters within the regenerating pygidium, and by 10 dpa, expression was evident in both the pygidium and the distal parapodia of the regenerated segment. These findings suggest that the re-emergence of germ cells in the pygidium may be supported by germ cell supply from the parapodia of the neighboring unamputated segment.
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