Enhancing the growth, hydrocolloid, and mineral content of Gracilaria verrucosa by selecting seedstock beyond local sources and various cultivation methods
The quality of seedlings is essential for optimal growth rate and seaweed quality. The availability of local seedlings often poses constraints on quality and sustainability. This research aims to determine the optimal cultivation method for Gracilaria verrucosa using seeds from multiple locations. Seedlings of G. verrucosa were sourced from Semarang (S), Brebes (B), and Demak (D). Observations were conducted by cultivating the three seedlings using the off-bottom (O), broadcast (B), and longline (L) methods. A completely randomised design with a combination of seedling origin and cultivation method (3x3), namely SO, SB, SL, BO, BB, BL, and DO, DB, DL, was conducted in Brebes Regency, Central Java. The highest growth rate of G. verrucosa was observed in SL (3.21±0.07 % day-1), while the lowest was observed in BB and DB. The broadcast cultivation method was shown to increase agar content across all seed sources, but this increase did not always correlate with gel strength. SB seedlings showed the best combination with high agar content (34.33±0.58%) and strong gel strength (294.7±6.05 g.cm?²), while SO produced the highest gel strength (324.7±5.21 g.cm?²) despite having lower agar content (26.65±0.80%). Integrating selective seed sources and optimised cultivation methods is crucial for enhancing growth, ensuring hydrocolloid quality, and achieving sustainable production of G. verrucosa.
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Apadym G., Ayliker V., Cengiz E., Saydam M., Kup N., Tirasoglu E. (2010). Analysis of metal contents of seaweed (Ulva lactuca) from Istanbul, Turkey by EDXRF. Turkish Journal of Fisheries and Aquatic Sciences, 10: 215-220.
Banik U., Mohiuddin M., Wahab M., Rahman M., Nahiduzzaman M., Sarkar S., Wong L., Asaduzzaman M. (2023). Comparative performances of different farming systems and associated influence of ecological factors on Gracilaria sp. seaweed at the south-east coast of the Bay of Bengal, Bangladesh. Aquaculture, 574: 739675.
Badraeni, Azis H., Tresnati J., Tuwo A. (2020). Seaweed Gracilaria changii as a bioremediator agent for ammonia, nitrite and nitrate in controlled tanks of Whiteleg Shrimp Litopenaeus vannamei. IOP Conference Series: Earth and Environmental Science, 564.
Bixler H.J., Porse H. (2011). A decade of change in the seaweed hydrocolloids industry. Journal of Applied Phycology, 23: 321-335.
Chen B., Zou D., Zhu M., Yang Y. (2017). Effects of CO2 levels and light intensities on growth and amino acid contents in red seaweed Gracilaria lemaneiformis. Aquaculture Research, 48: 2683-2690.
Chaqmaqchee F., Baker A., Salih N.F. (2017). Comparison of various plastics wastes using X-ray fluorescence. American Journal of Materials Synthesis and Processing, 2(2): 24-27.
Debbarma J., Rao B.M., Murthy L.N., Mathew S., Venkateshwarlu G., Ravishankar C.N. (2016). Nutritional profiling of the edible seaweeds Gracilaria edulis, Ulva lactuca and Sargassum, sp. Indian Journal of Fisheries, 63(3): 81-87.
FAO. (2018). The Global Status of Seaweed Production, Trade and Utilization Vol. 124. Roma.
Filippini M., Baldisserotto A.M., Simonetta., Giorgio F.R., Silva G., Domenico V., Giorgia B., Raissa., Stefano M., Silvia V. (2021). Heavy metals and potential risks in edible seaweed on the market in Italy. Chemosphere, 263: 127983.
Hayashi L., Leonardi P.I., Critchley A.T. (2017). The cultivation of Kappaphycus and Eucheuma in tropical and subtropical waters. Advances in Botanical Research, 78: 121-156.
Hurtado A.Q., Neish I.C., Critchley A.T. (2019). Phyconomy: The seaweed industry in the Asia-Pacific region. Journal of Applied Phycology, 31(2): 797-818.
Ho C.L., Lim Y-y., Lee W-K. (2019). PH affects growth, physiology, and agar properties of agarophyte Gracilaria changii (Rhodophyta) under low light intensity from Morib, Malaysia. Regional Studies in Marine Science, 30: 100738.
Indonesian National Standard (SNI). 2011. Water quality: Determination of nitrate with spectrophotometric method using cadmium reduction. SNI 6989.79:2011. National Standardization Agency of Indonesia (BSN), Jakarta.
Indonesian National Standard (SNI). (2004). Water quality—Determination of nitrite by spectrophotometric method. SNI 06-6989.9:2004. National Standardization Agency of Indonesia (BSN), Jakarta.
Indonesian National Standard (SNI). (2005). Water quality—Determination of ammonia with spectrophotometric phenate method. SNI 06-6989.30:2005. National Standardization Agency of Indonesia (BSN), Jakarta.
Indonesian National Standard (SNI). (2005(. Water quality—Determination of orthophosphate by spectrophotometric ascorbic acid method. SNI 06-6989.31:2005. National Standardization Agency of Indonesia (BSN), Jakarta.
Jha A.K., Panda S.K., Kishore P., Mathew S., Ravishankar C.N. (2021). Trace-minerals and lipid quality indices in seaweeds growing at Okhta, India: A health risk assessment. Regional studies in Marine Science, 47: 101966.
Kim J.K., Yarish C., Pereira R. (2016). Tolerances to hypo-osmotic and temperature stress in native and invasive species of Gracilaria (Rhodophyta). Phycologia, 55(3): 257-264.
Lee W., Lim Y., Leow A., Namasivayam P., Abdullah J., Ho C. (2016). Factors affecting yield and geling properties of agar. Journal of Applied Phycology, 29: 1527-1540.
Mustafa A., Athirah A., Ratnawati E., Paena M., Kamariah K., Asaad A., Tarunamulia T., Asaf R., Syaichudin M., Taukhid I. (2024). Identifying the environmental factors that determine the agar content of seaweed Gracilaria verrucosa in acid sulfate soil-affected brackishwater ponds. Polish Journal of Environmental Studies, 33(4): 4673-4686.
Noor M., Hidayat N., Mohamad M., Abdullah N., Rusly S., Saad S., Mukai Y., Susanti D. (2021). Growth of Gracilaria manilaensis Yamamoto et Trono (Rhodophyta) under different light intensities, salinities and pH. Borneo Journal of Marine Science and Aquaculture (BJoMSA), 5(2): 53-56.
Peñalver R., Lorenzo J.M., Ros G., Amarowicz R., Pateiro M. (2020). Seaweeds as a Functional Ingredient for a Healthy Diet. Marine Drugs, 18(6): 301.
Rejeki S., Ariyati R., Widowati L.L., Bosma R.H. (2018). The effect of three cultivation methods and two seedling types on growth, agar content and gel strength of Gracilaria verrucosa. Egyptian Journal of Aquatic Research, 44(1): 65-70.
Ricker W.E. (1975). Computation and interpretation of biological statistics of fish populations. Fisheries Research Board of Canada, Bulletin, 191: 1-382.
Sawant O., Jaiswar S. (2024). Assessing the nitrate and phosphate uptake kinetics potential and growth performance of Gracilaria corticata var. cylindrica in shrimp farm water (SFW). Discover Oceans, 1: 7.
Siregar R., Permadi A., Wijaya V. (2024). Physicochemical characteristics of agar from Gracilaria verrucosa at different locations. Postharvest and Marine Biotechnology Journal of Fisheries and Marine Affairs, 19(2): 93.
Smith J.L., Summers G., Wong R. (2010). Nutrient and heavy metal content of edible seaweeds in New Zealand. New Zealand Journal of Crop and Horticultural Science, 38: 19-28.
Weizhou C. (2012). Effects of different temperature and salinity on growth and biochemical constituents of Gracilaria chouae. South China Fisheries Science, 8(2): 51-57.
Wong K.H., Cheung P.C.K. (2000). Nutritional evaluation of some subtropical red and green seaweeds Part I – proximate composition, amino acid profiles and some physico-chemical properties. Food Chemistry, 71: 475-482.
Yarnpakdee S., Benjakul S., Kingwascharapong P. (2015). Physico-chemical and gel properties of agar from Gracilaria tenuistipitata from the lake of Songkhla, Thailand. Food Hydrocolloids, 51: 217-226.
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