Growth performance and nutrient utilization of glass catfish (Kryptopterus lais) larvae in response to varying dietary protein levels
Downloads
This study evaluated the effects of varying dietary protein levels (35, 40, 45, and 50%) on growth, feed utilization, and body composition in Glass catfish, Kryptopterus lais, larvae over 30 days. Larvae with an initial average weight of 0.15±0.01 g were distributed into 12 aquaria at a density of 50 individuals per tank. Fish were fed four times daily to apparent satiation. The results indicated that weight gain (WG) increased with dietary protein levels up to 40%, beyond which WG declined. Similarly, the feed conversion ratio was lowest in fish fed the 40% protein diet. The protein efficiency ratio was significantly higher in K. lais larvae fed the 40% protein diet compared to those fed diets containing 45 and 50% protein. Body composition analysis revealed that larvae fed the 40% protein diet exhibited the highest protein content, while those fed the 35% protein diet had a significantly elevated lipid content. Broken-line regression analysis, based on the specific growth rate, estimated the optimal dietary protein requirement for maximal growth performance to be 42.89%. These findings provide a quantitative basis for formulating diets that effectively meet the protein requirements of K. lais larvae.
Downloads
Ahmed I., Ahmad I. (2020). Effect of dietary protein levels on growth performance, hematological profile, and biochemical composition of fingerlings of rainbow trout, Oncorhynchus mykiss, reared in the Indian Himalayan region. Aquaculture Reports, 16: 100268.
Ahmed I., Maqbool A. (2017). Effects of dietary protein levels on the growth, feed utilization and haemato-biochemical parameters of freshwater fish. Cyprinus Carpio Var Specularis. Fisheries and Aquaculture Journal, 8: 187.
Ali M.R., Afzal M., Khan M.F., Naqvi S.M.H.M., Akhtar S. (2014). Dietary protein requirement of giant river catfish, Sperata seenghala (sykes), determined using diets of varying protein level. Pakistan Journal of Nutrition, 13(3): 151-156.
Ali M.R., Basharat H., Ahmed A., Fakhar M., Khan A. (2024). Determination of optimum dietary crude protein requirement for maximum growth of African catfish (Clarias gariepinus). Sarhad Journal of Agriculture, 40(2): 595-602.
Anizah M.R., Saad C.R., Kamarudin M.S., Rahim A.A. (2017). Effect of dietary protein and protein energy ratio on the growth performance of lemon fin barb hybrid (Hypsibarbus wetmorei X Puntius gonionotus) larvae. Iranian Journal of Fisheries Sciences, 16(2): 711-721.
AOAC. (2005). Official method of analysis. 18th Edition, Association of Officiating Analytical Chemists, Washington DC, Method 935.14 and 992.24.
Arisuryanti T., Nikmah B.U., Kasayev T., Hakim L. (2020). Determination of species boundaries of Selais fish from Arut River, Central Kalimantan based on 16S mitochondrial gene using Bayesian approach. In: BIO Web of Conferences, 28: 01003. EDP Sciences.
Biswas P., Jena A.K., Patel A.B., Pandey P.K. (2020). Dietary protein requirement of Indian Butter catfish, Ompok bimaculatus (Bloch) fingerlings. Journal of Applied Aquaculture, 32(2): 107-123.
Cai L.S., Wang L., Song K., Lu K.L., Zhang C.X., Rahimnejad S. (2020). Evaluation of protein requirement of spotted seabass (Lateolabrax maculatus) under two temperatures, and the liver transcriptome response to thermal stress. Aquaculture, 516: 734615.
Chandan C.S.S., Roy P.U.J.A., Khatun F.A.H.I.M.A., Roy N.C. (2021). Effect of dietary protein on growth, survival and cannibalism of larval Striped Snakehead, Channa striata (Bloch, 1793). Asian Fisheries Science, 34(3): 236-242.
Chang J., Niu H.X., Jia Y.D., Li S.G., Xu G.F. (2018). Effects of dietary lipid levels on growth, feed utilization, digestive tract enzyme activity and lipid deposition of juvenile Manchurian trout, Brachymystax lenok (Pallas). Aquaculture Nutrition, 24: 694-701.
Cho S.H., Lee S.M., Lee J.H. (2005). Effect of dietary protein and lipid level on growth and body composition of juvenile turbot (Scophthalmus maximus L.) reared under optimum salinity and temperature conditions. Aquaculture Nutrition, 11: 235-250.
Cho J.H., Lee S., Lee B.J., Hur S.W., Kim K.W., Son M.H., Yoo D.J. (2021). A preliminary study of dietary protein requirement of juvenile marbled flounder (Pseudopleuronectes yokohamae). Animal Nutrition, 7(2): 548-555.
Chou R.L., Su M.S., Chen H.Y. (2001). Optimal dietary protein and lipid levels for juvenile cobia (Rachycentron canadum). Aquaculture, 193(1-2): 81-89.
Debnath D., Pal A.K., Sahu N.P., Yengkokpam S., Baruah K., Choudhury D., Venkateshwarlu G. (2007). Digestive enzymes and metabolic profile of Labeo rohita fingerlings fed diets with different crude protein levels. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 146(1): 107-114.
Deng J., Zhang X., Bi B., Kong L., Kang B. (2011). Dietary protein requirement of juvenile Asian red-tailed catfish Hemibagrus wyckioides. Animal Feed Science and Technology, 170(3-4): 231-238.
Efrizal., Andre S.R., Lubis A.S., Eriza M. (2023). The quality of artificial feed test by adding thyroxine hormones on the growth of selais fish Kryptopterus lais (Bleeker, 1851). Aquaculture, Aquarium, Conservation and Legislation, 16(3): 1410-1417.
González-Rodríguez Á., Celada J.D., Carral J.M., Sáez-Royuela M., Fuertes J.B. (2014). Effects of varying protein level in practical diets on survival, growth, feed utilization and body composition of juvenile tench (Tinca tinca L.). Aquaculture International, 22: 1723-1735.
Hadi K., Suharman I., Hasan B., Rosyadi R., Caipang C.M.A. (2024). Shrimp head protein hydrolysate as a potential feed attractant for the Asian redtail catfish (Hemibagrus nemurus) larvae. Egyptian Journal of Aquatic Biology and Fisheries, 28(5): 1755-1769.
Hardy R.W., Gatlin III D.M. (2002). Nutritional strategies to reduce nutrient losses in intensive aquaculture. Avances en Nutrición Acuícola. VI. Memorias del VI Simposium Internacional de Nutricio´n Acu?´cola. Cancun, Quintana Roo, Mexico. pp: 23-34.
Hepher B. (1988). Nutrition of pond fishes. Cambridge: Cambridge University Press. New York, Sydney. 208 p.
Islam M.S., Tanaka M. (2004). Optimization of dietary protein requirement for pond-reared mahseer, Tor putitora Hamilton (Cypriniformes: cyprinidae). Aquaculture Research, 35: 1270-1276.
Jang B.I., Olowe O.S., Cho S.H. (2022). Evaluation of the optimal protein required in granulated microdiets for rockfish (Sebastes schlegeli) larvae. Aquaculture Nutrition, 2022(1): 2270384.
Jauncey K. (1982). The effects of varying dietary protein level on the growth, food conversion, protein utilization and body composition of juvenile tilapias (Sarotherodon mossambicus). Aquaculture, 27(1): 43-54.
Jayant M., Muralidhar A.P., Sahu N.P., Jain K.K., Pal A.K., Srivastava P.P. (2018). Protein requirement of juvenile striped catfish, Pangasianodon hypophthalmus. Aquaculture International, 26: 375-389.
Khan M.S., Ang K.J., Ambak M.A., Saad C.R. (1993). Optimum dietary protein requirement of a Malaysian freshwater catfish, Mystus nemurus. Aquaculture, 112(2-3): 227-235.
Kim L.O., Lee S.M. (2005). Effects of dietary protein and lipid levels on growth and body composition of bagrid catfish, Pseudobagrus fulvidraco. Aquaculture, 243: 323-329.
Kim S., Lee K. (2009). Dietary protein requirement of juvenile tiger puffer (Takifugu rubripes). Aquaculture, 287: 219-222.
Kpogue D.N., Ayanou G.A., Toko I.I., Mensah G.A., Fiogbe E.D. (2013). Influence of dietary protein levels on growth, feed utilization and carcass composition of snakehead, Parachanna obscura (Günther, 1861) fingerlings. International Journal of Fisheries and Aquaculture, 5(5): 71-77.
Jiang S., Mo X., Zhou F., Huang J., Yang Q., Yang L., Jiang S. (2022). Effect of different dietary levels of protein on growth performance of Penaeus monodon families. Pakistan Journal of Zoology, 54: 129-136.
Lee S.M., Jeon I.G., Lee J.Y. (2002). Effects of digestible protein and lipid levels in practical diets on growth, protein utilization and body composition of juvenile rockfish (Sebastes schlegeli). Aquaculture, 211(1-4): 227-239.
Leksono T., Edison., Irasari., Ikhsan M.N. (2020). The effect of different variety of fire-woods on smoking of selais catfish (Cryptopterus bicirchis). In IOP Conference Series: Earth and Environmental Science, 430(1): 012002.
Liu H., Dong X., Tan B., Du T., Zhang S., Yang Y., Chi S., Yang Q., Liu H. (2021). Effects of dietary protein and lipid levels on growth, body composition, enzymes activity, expression of IGF-1 and TOR of juvenile northern whiting, Sillago sihama. Aquaculture, 533: 736166.
Ma H., Liu K., Feng X., Huang H., Wu Y., Xie N. (2021). The optimal dietary protein and lipid levels for juvenile hybrid sunfish (lepomis cyanellus?× Lepomis macrochirus?). Aquaculture Nutrition, 27(3): 841-849.
Mir I.N., Srivastava P.P., Bhat I.A., Jaffar Y.D., Sushila N., Sardar P., Kumar S., Muralidhar A.P., Jain K.K. (2020). Optimal dietary lipid and protein level for growth and survival of catfish Clarias magur larvae. Aquaculture, 520: 734678.
Mohseni M., Pourkazemi M., Karaminasab M., Rastravan M. (2019). Effect of different dietary protein levels on growth, carcass biochemical composition and apparent digestibility coefficient in juvenile Caspian trout (Salmo trutta caspius). Iranian Fisheries Science Research Institute, 28(2): 165-177.
Muchtar A., Rosyadi. (2020). Effect stocking density on growth and survival rate of larval selais fish (Kryptopterus lais) cultured in recirculation system. In Proceedings of the Second International Conference on Science, Engineering and Technology (ICoSET 2019). 254-257.
Nurmayani., Tang U.M., Putra R.M., Nofrizal., Patadjai R.S. (2020). Cultivation of selais fish (Kryptopterus lais) in floating net cages in kayangan lake Pekanbaru. International Journal of Oceans and Oceanography, 14: 285-294.
Park I.S., Lee S., Yoo G.Y. (2022). Impact of dietary protein levels on growth, feed utilization, body composition, and hematological characteristics of juvenile hybrid pufferfish (Takifugu obscurus× T. rubripes). Aquaculture Reports, 22: 100994.
Qian J., Xiao L., Feng K., Li W., Liao C., Zhang T., Liu J. (2022). Effect of dietary protein levels on the growth, enzyme activity, and immunological status of Culter mongolicus fingerlings. Plos One, 17(2): e0263507.
Rahimnejad S., Dabrowski K., Izquierdo M., Malinovskyi O., Kolá?ová J., Policar T. (2021). Effects of dietary protein and lipid levels on growth, body composition, blood biochemistry, antioxidant capacity and ammonia excretion of European grayling (Thymallus thymallus). Frontiers in Marine Science, 8: 715636.
Ren M., Ji K., Liang H., Ge X., Mi H. (2017). Dietary protein requirement of juvenile ide, Leuciscus idus in relation to growth performance, whole-body composition and plasma parameters. The Journal of Aquaculture – Bamidgeh, 69(1): 1424.
Rotili D.A., Rossato S., de Freitas I.L., Martinelli S.G., Radünz Neto J., Lazzari R. (2018). Determination of methionine requirement of juvenile silver catfish (Rhamdia quelen) and its effects on growth performance, plasma and hepatic metabolites at a constant cystine level. Aquaculture Research, 49(2): 858-866.
Singh R.K., Desai A.S., Chavan S.L., Khandagale P.A. (2009). Effect of water temperature on dietary protein requirement, growth and body composition of Asian catfsh, Clarias batrachus fry. Journal of Thermal Biology, 34: 8-13.
Thamrin S. (2020). The development of smoked selais fish as a gastronomic tourist attraction in Riau Province. The Journal Gastronomy Tourism, 7(2): 62-67.
Wang J., Jiang Y., Li X., Han T., Yang Y., Hu S., Yang M. (2016). Dietary protein requirement of juvenile red spotted grouper (Epinephelus akaara). Aquaculture, 450: 289-294.
Wang C.A., Hu G., Sun P., Gu W., Wang B., Xu Q., Liu H. (2018). Effects of dietary protein at two lipid levels on growth, gonadal development, body composition and liver metabolic enzymes of brown trout (Salmo trutta fario) broodstock. Aquaculture Nutrition, 24(5): 1587-1598.
Wang J.T., Han T., Li X.Y., Yang Y.X., Yang M., Hu S.X., Jiang Y.D., Harpaz S. (2017). Effects of dietary protein and lipid levels with different protein?to?energy ratios on growth performance, feed utilization and body composition of juvenile red?spotted grouper, Epinephelus akaara. Aquaculture Nutrition, 23(5): 994-1002.
Wang Z., Li S., Zhou Q., Zhang J., Li Y., Li Y., Li Z., Huang G. (2023). Effects of different protein and lipid levels on the growth performance and intestinal microflora of loach (Paramisgurnus dabryanus). Animal Nutrition, 13: 229-239.
Wu X., Gatlin III D.M. (2014). Effects of altering dietary protein content in morning and evening feedings on growth and ammonia excretion of red drum (Sciaenops ocellatus). Aquaculture, 434: 33-37.
Yan X., Yang J., Dong X., Tan B., Zhang S., Chi S., Yang Q., Liu H., Yang Y. (2021). Optimum protein requirement of juvenile orange-spotted grouper (Epinephelus coioides). Scientific Reports, 11(1): 6230.
Ye C., Wu Y., Sun Z., Wang A. (2017). Dietary protein requirement of juvenile obscure puffer, Takifugu obscurus. Aquaculture Research, 48(5): 2064-2073.
Zeng B., Wang W., Dong Y. (2020). Dietary protein requirement of the high altitude's representative teleost juveniles Schizopygopsis younghusbandi (Cypriniformes: Cyprinidae). Aquaculture Research, 51(7): 2852-2862.
Zhang Y., Liang X., Zhan W., Han M., Liu F., Xie Q., Guo D., Ghen L., Lou B. (2022). Effects of dietary protein levels on growth performance, muscle composition and fiber recruitment of juvenile small yellow croaker (Larimichthys polyactis). Aquaculture Reports, 27: 101335.
Zhang Y., Sun Z., Wang A., Ye C., Zhu X. (2017). Effects of dietary protein and lipid levels on growth, body and plasma biochemical composition and selective gene expression in liver of hybrid snakehead (Channa maculata?× Channa argus?) fingerlings. Aquaculture, 468: 1-9.
Copyright (c) 2025 International Journal of Aquatic Biology

This work is licensed under a Creative Commons Attribution 4.0 International License.







