Mortality rate and immune responses of rainbow trout (Oncorhynchus mykiss) infected with Yersinia ruckeri subsequent to feeding on diet supplemented with Ducrosia anethifolia essential oil

Fatemeh Dehghan, Arya Vazirzadeh, Siyavash Soltanian, Akbar Karami, Mostafa Akhlaghi


Application of the immunostimulant is the most promising method for controlling diseases in aquaculture. In this study, the mortality rate and immune responses of rainbow trout (Oncorhynchus mykiss) fed on diet supplemented with Ducrosia anethifolia essential oil was investigated after challenging with Yersinia ruckeri. The essential oil mixed with sunflower oil at different concentrations (0.001, 0.01 and 0.1%) and the commercial food was coated with this oil. Fish were fed with diets for 8 weeks and infected with Y. ruckeri at the ending of feeding trial. Serum protein, albumin, globulin and lysozyme and bactericidal activity of challenged fish were evaluated one week after injection and mortality were counted till day 10. The results showed that albumin had not differed among treatments. The highest level of the protein and globulin were found in control group. Serum lysozyme activity showed no difference between groups. The highest and lowest serum bactericidal activity was observed in 0.001% and control group, respectively. The mortality rates in infected fish were as 55% in control group, 40% in 0.001%, 70% in 0.01% and 70% in 0.1% treatment. Lowest rate of mortality was observed in group 0.001%, while began two days earlier than other groups.


Immunostimulant, Lysozyme, Bactericidal, Blood protein.

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Biller-Takahashi J., Takahashi L., Pilarski F., Sebastião F., Urbinati E. (2013). Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887). Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 65: 1745-1751.

Bowden T.J., Butler R., Bricknell I.R. (2004). Seasonal variation of serum lysozyme levels in Atlantic halibut (Hippoglossus hippoglossus L.). Fish and Shellfish Immunology, 17: 129-135.

Hajhashemi V., Rabbani M., Ghanadi A., Davari E. (2010). Evaluation of antianxiety and sedative effects of essential oil of Ducrosia anethifolia in mice. Clinics, 65: 1037-1042.

Hajibeglou A., Sudagar M. (2010). Immune response of common carp (Cyprinus carpio) fed with herbal immunostimulants diets. Agricultural Journal, 5(3): 63-172.

Harikrishnan R., Balasundaram C., Heo M.S. (2011a). Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture, 317: 1-15.

Harikrishnan R., Balasundaram C., Heo M.S. (2011b). Influence of diet enriched with green tea on innate humoral and cellular immune response of kelp grouper (Epinephelus bruneus) to Vibrio carchariae infection. Fish and Shellfish Immunology, 30: 972-979.

Harikrishnan R., Balasundaram C., Kim M.C., Kim J.S., Han Y.J., Heo M.S. (2009). Innate immune response and disease resistance in Carassius auratus by triherbal solvent extracts. Fish and Shellfish Immunology, 27: 508-515.

Ispir U., Gokhan H.B., Ozcan M., Dorucu M., Saglam N. (2009). Immune response of rainbow trout (Oncorhynchus mykiss) to selected antigens of Yersinia ruckeri. Acta Veterinaria Brno, 78: 145-150.

Janssen A.M., Scheffer J.J.C., Svendsen A.B., Aynehchi Y. (1984). The essential oil of Ducrosia anethifolia (DC.) Boiss. Pharmaceutisch Weekblad, 6(4): 157-160.

Parry R.M., Chandan R.C., Shahani K.M. (1965). A rapid and sensitive assay of muramidase. Experimental Biology and Medicine, 119(2): 384-386.

Pratheepa V., Ramesh S., Sukumaran N. (2010). Immunomodulatory effect of Aegle marmelos leaf extract on freshwater fish Cyprinus carpio infected by bacterial pathogen Aeromonas hydrophila. Journal of Pharmaceutical Biology, 48: 1224-1239.

Rao V.Y., Das B.K., Jyotyrmavee P., Chakrabarthi, R. (2006). Effect of Achyranthes aspera on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila. Fish and Shellfish Immunology, 20: 263-273.

Shahabipour S., Firuzi O., Asadollahi M., Faghihmirzaei E., Javidnia K. (2013). Essential oil composition and cytotoxic activity of Ducrosia anethifolia and Ducrosia flabellifolia from Iran. Journal of Essential Oil Research, 25: 160-163.

Sheikhzadeh N., Nofouzi K., Delazar A., Oushani A.K. (2011). Immunomodulatory effects of decaffeinated green tea (Camellia sinensis) on the immune system of rainbow trout (Oncorhynchus mykiss). Fish and Shellfish Immunology, 31: 1268-1269.

Soltani M., Sheikhzadeh N., Ebrahimzadeh-Mousavi H., Zargar A. (2010). Effects of Zataria multiflora essential oil on innate immune responses of common carp (Cyprinus carpio). Journal of Fisheries and Aquatic Science, 5: 191-199.

Zhang G., Gong S., Yu D., Yuan H. (2009). Propolis and Herba Epimedii extracts enhance the non-specific immune response and disease resistance of Chinese sucker, Myxocyprinus asiaticus. Fish and Shellfish Immunology, 26: 467-472.



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