Isolation and characterization of lactic acid bacteria from the fish intestine for application as probiotics in young common carp Cyprinus carpio L. diet
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The current study was conducted to investigate the possibility of using Lactic acid bacteria LAB isolated from the intestines of common carp, Cyprinus carpio, as dietary probiotics to enhance the nutrition and growth of common carp juveniles. The bacteria were cultured on MRS agar and their cultural and biochemical characteristics were confirmed. The isolated probiotic bacteria were used as dietary supplements at concentrations of 106, 107, and 108 and their effect on the nutrition and growth of common carp fingerlings was evaluated. The results showed clear positive effects of adding the probiotic Lactobacilli in fish feed, with a relative growth increase from 105.26 to 178.14% with increasing probiotic concentration compared to the control. The feed conversion ratio also improved from 2.94 to 1.83 with increasing levels of probiotics compared to the control. In addition, all other indicators improved, including weight gain, specific growth rate, protein efficiency ratio, digestibility, and beneficial intestinal flora, with increasing probiotic concentration in the feed compared to the control, with a significant difference. It was concluded that LAB isolated from the intestines of common carp.can be effectively used as probiotics in common carp C. carpio fingerlings feed, with positive results.
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Adineh H., Jamarion H., Shandi J., Alizadeh M. (2013). Effect of Bacillus spp. probiotic on growth and feed performance of rainbow trout Oncorhynchus mykiss larvae. Bulgarian Journal of Veterinary Medicine, 16(1): 29-36.
Al-Hassani S.T., Mustafa S.A. (2022). Efficiency of symbiotic as feed additives on growth performance, survival rate and health status in Common carp challenged with Saprolegnia spp. Iraqi Journal of Agricultural Sciences, 53(2): 397-405.
Al-Asha'ab M.H., Mohammad S.D., Al-fathly M.K., Neamah Y.J. (2014). Effect of Using Probiotics with Prebiotics in Growth Indicia and Some Physiological Characters for Fingerlings Common Carp Cyprinus carpio L., Journal of Biotechnology Research Center, 8(2): 44-50.
Al-Dubakel A.Y., Al-Hamadany Q.H., Mohamed A.A. (2015). Effect of local probiotic (Iraqi probiotic) on the growth of common carp Cyprinus carpio L. youngs. Journal of Basrah Researches (Sciences), 41(B.3): 57-69.
Al-Faragi J.K.H., Alsaphar S.A.A. (2012). Isolation and identification of Bacillus subtilus as probiotic) from intestinal microflora of common carp Cyprinus carpio L. Proceeding of the Eleventh Veterinary Scientific Conference, 355-361.
Al-Janabi M.F., Al-Noor J.M., Al-Dubakel A.Y. (2022). Evaluation of Thepax and Bio Boost for stimulating microorganisms in the intestines of common carp Cyprinus carpio. Bionatura, 1-13.
Al-Janabi M.F., Al-Noor J.M., Al-Dubakel A.Y. (2021). Evaluation of theca and endo Vit. C as growth promoters for common carp Cyprinus Carpio. Natural Volatiles and Essential Oils, 8(6): 1976-1992.
Amit P.A., Khairnar S.O., Tyagi A. (2021). Effect of dietary supplementation of probiotic bacteria Lactobacillus plantarum on growth and proximate composition of Cyprinus carpio Fingerlings. National Academy Science Letters, 44(6): 495-502.
Amit P.A., Tyagi A., Khairnar S.O. (2022). Oral feed-based administration of Lactobacillus plantarum enhances growth, haematological and immunological responses in Cyprinus carpio. Emerging Animal Species, 3: 1-9.
Andrews W. (1992). Manual of food quality control. 1. Microbiological analysis. FAO Food and Nutrition Paper, 14(4 Revis 1): 1-338.
Anguiano M., Pohlenz C.A.B., Gatlin D.M. (2013). The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum (Sciaenops ocellatus) and hybrid striped bass (Morone chrysops × M. saxatilis). British Journal of Nutrition, 109: 623-629.
Assan D., Kofi F., Kuebutornye A., Hlordzi V., Chen H., Mraz J., Mustapha U.F., Abarike E.D. (2022). Effects of probiotics on digestive enzymes of fish (finfish and shellfish); status and pros-pects: A mini-review. Comparative Biochemistry and Physiology, B 257: 110653.
Awan J.A., Rahman S.U. (2005). Microbiology Manual. Unitech Communications, Faisalabad. pp: 49-51.
Azevedo R.V., Filho J.C.F., Cardoso L.D.C., Mattos D.C.M., Júnior M.V.V., Andrade D.R. (2015). Economic evaluation of prebiotics, probiotics and symbiotic in juvenile Nile tilapia. Revista Ciencia Agronomica, 46(1): 72-79.
Beiwi D.A., Al-Hisnawi A. (2020). Effect of Bacillus subtilis as probiotic on intestinal microbiota and growth performance of common carp (Cyprinus carpio). In AIP Conference Proceedings. AIP Publishing LLC, 2290(1): 030004.
Bennani S., Mchiouer K., Rokni Y., Meziane M. (2017). Characterization and Identification of Lactic Acid Bacteria Isolated from Morrocan Raw Cow’s Milk. Journal of Materials and Environmental Sciences, 8(S): 4934-4944.
Bintsis T. (2018). Lactic Acid Bacteria: Their Applications in Foods. Journal of Bacteriology and Mycology, 6(2): 89-94.
Boulares M., Mejri L., Hassouna M. (2011). Study of the microbial ecology of wild and aquacultured Tunisian Fresh Fish. Journal of Food Protection, 74(10): 1762-1768.
Chizhayeva A., Amangeldi, A., Oleinikova Y., Alybaeva A., Sadanov A. (2022). Lactic acid bacteria as probiotics in sustainable development of aquaculture. Aquatic Living Resources, 35(10): 1-17.
Cláudia R., Serra A., Enes O.P., Tavares F. (2021). Gut microbiota dynamics in carnivorous European seabass (Dicentrarchus labrax) fed plant-based diets. Scientific Reports, 11: 447.
Dhanaraj M., Haniffa M.A., Arun S., Singh V., Jesu A., Ramakrishanan C., Seetharaman S., Arthimangu R. (2010). Effect of probiotic on growth performance of Kio carp (Cyprinus carpio). Journal of Applied Aquaculture, 22: 202-209.
Djauhari R., Widanari S., Supraydi M.A., Zairnijr M. (2017). Growth performance and health status of common carp (Cyprinus carpio) supplemented with prebiotic from sweet potato (Ipomoea batatas L.) extract. Pakistan Journal of Nutrition, 16(3): 155-163.
Ekundayo F.O. (2014). Isolation and identification of lactic acid bacteria from rhizosphere soils of three fruit trees, fish and ogi. International Journal of Current Microbiology and Applied Sciences, 3(3): 991-998.
Feng J., Liu S., Zhu C.L Cai Z.L Cui W.L., Chang X., Nie G. (2022). The effects of dietary Lactococcus spp. on growth performance, glucose absorption and metabolism of common carp, Cyprinus carpio L. Aquaculture, 546: 737394
Gerhardt P. (1993). Manual of Methods for General Bacteriology, 1st ed. American Society for Microbiology, Washington D.C. pp: 303-304.
Goran S.M.A., Omar S.S., Anwer A.Y. (2017). Assessment of yeast as a dietary additive on haematology and water quality of common carp in a recirculating aquaculture system. In AIP conference proceedings. AIP Publishing LLC, 1888(1): 020023.
Guerreiro I., Serra C.L., Oliva-Teles A., Enes P. (2018). Short communication: gut microbiota of European sea bass (Dicentrarchus labrax) is modulated by short-chain fructooligosaccharides and xylooligo-saccharides. Aquaculture International, 26(1): 279-288.
Guerreiro I., Serra C.R., Ferreira P.P., Oliva-Teles A., Enes P. (2017). Prebiotics effect on growth performance, hepatic intermediary metabolism, gut microbiota and digestive enzymes of White Sea bream (Diplodus sargus). Aquaculture Nutrition, 24(1): 153-163.
Gupta A.; Gupta P., Dhawan A. (2014). Dietary supplementation of probiotics affects growth, immune responses and diseases resistance of Cyprinus carpio fry. Fish and Shellfish Immunology, 41(2): 113-119.
Gupta R., Jeevaratnam K., Fatima A. (2018). Lactic Acid Bacteria: Probiotic Characteristics, Selection Criteria, and Its Role in Human Health. Journal of Emerging Technologies and Innovative Research, 5(10): 411-424.
Harley J.P., Prescott L.M. (2002). Laboratory exercises in microbiology. 5th ed., The McGraw–Hill Companies, U.S.A. 449 p.
Hayek S.A., Gyawali R., Aljaloud S.O., Krastanov A., Ibrahim S.A. (2019). Cultivation media for lactic acid bacteria used in dairy products. Journal of Dairy Research, 86(4): 490-502.
Herwing N. (1979). Handbook of drugs and chemicals used in the treatment of fish diseases: A manual of fish pharmacology and materia medica. Thomas, Springfield. 272 p.
Iman M.K., Wafaa T., Abass T., Elham S., Awaad S., Mohammad M.N., Kawthc E., Gamal A., lbrahim A., Sade, Z., Elsayed H.S. (2013). Evaluation of Lactobacillus plantarum as a probiotic in aquaculture: Emphasis and growth performance and innate immunity. The Journal of Applied Sciences Research, 9(1): 572-582.
Imran S.M., Ail A.H., Najim S.M. (2019). Effect of Dietary Prebiotic Safmannan and Bio-antibiotic Fluconazole on some Growth and Haemato -immunological Parameters of Common Carp Cyprinus carpio L. Basrah Journal of Agricultural Sciences, 32(2): 176-192.
Ismael D.A.A.N. (2022). Isolation Probiotic Bacteria and Identification in Improving Immune Response of carp fish in Comparison with Commercial Product. Al-Kunooze Scientific Journal, 4(1):1.
Jibing M. (1993). Bioenergetics feed intake and energy partitioning. In: J.C. Rankin, F.B. Jensen, (Eds.), Fish physiology. London: Chapman and Hall. pp: 1-44.
Kafi Z.Z., Peyghan R., Modaresi S.M.H., Motevaseli E., Ghorbanpour M. (2022). Probiotic properties of some lactic acid bacteria isolated from intestine of cultured common carp, Cyprinus carpio, in Khuzestan Province, Iranian Veterinary Journal, 17(1): 86-96.
Kord M.I., Srour T.M., Omar E.A., Farag A.A., Nour A.A.M., Khalil H.S. (2021). The immunostimulatory effects of commercial feed additives on growth performance, non-specific immune response, antioxidants assay, and intestinal morphometry of Nile tilapia, Oreochromis niloticus. Frontiers in Physiology, 25(12): 627499.
Kumaree K.K., Akbar A., Anal A.K. (2015). Bioencapsulation and application of Lactobacillus plantarum isolated from catfish gut as an antimicrobial agent and additive in fish feed pellets. Annals of Microbiology, 65(3): 1439-1445.
Lambuk F., Mazlan N., Thung T.Y., New C.Y., Rinai K.R., Son R. (2022). A review of lactic acid bacteria isolated from marine animals: their species, isolation site and applications. Food Research, 6(1): 311-323
Lund R. (2021). Effects of functional ingredients from yeast in diets for Atlantic salmon (Salmo salar), from two genetic backgrounds, on growth performance and Nutrientutilization. Department of Animal and Aquacultural Sciences (IHA) Faculty of Biosciences. Master’s Thesis. 49 p.
Magouz F.I., Ismail A., Radwan I.A., Soltan H.O., El-Keredy A. (2023). Synbiotic Lactic Dry® enhanced the growth performance, growth-related genes, intestinal health, and immunity of Nile tilapia reared in inland brackish groundwater. Annals of Animal Science, 1-29.
Majeed K.R., Ghadban A.K.G., Saleh F.M. (2017). A study of inhibition Activity of Lactobacillus spp. Against fungi and Aflatoxin B1 in vitro. Syrian Journal of Agricultural Research, 4(3): 65-79.
Maniat M., Salati A.P., Zanguee N., Mousavi S.M., Hoseinifar S.H. (2023). Effects of dietary Pediococcus acidilactici and Isomaltooligosaccharide on growth performance, immunity, and antioxidant defense in juvenile common carp. Aquaculture Nutrition, 1-8.
Meng X.L., Hu W.P., Wu S.K., Zhu Z.X., Lu R.H., Yang GK. (2019). Chinese yam peel enhances the immunity of the common carp (Cyprinus carpio L.) by improving the gut defence barrier and modulating the intestinal microflora. Fish and Shellfish Immunology, 95: 528-537.
Mohammed M.A., Al-Safao R.J.M. (2013). The effect of adding some probiotic bio-boosters in the attic to the performance of carp common in glass basins. Rafidain Agriculture Magazine, 41(2): 99-111.
Nieto-Domínguez M., de-Eugenio L.I., York-Durán M.J., Rodríguez-Colinas B., Plou F.J., Chenoll E., Pardo E., Codoñer F., Jesús-Martínez M. (2017). Prebiotic effect of xylooligosaccharides produced from Birchwood xylan by a novel fungal GH11 xylanase. Food Chemistry, 232: 105-113.
Nimalan N., Sørensen S.L., Fe?ckaninova A., Koscova J., Mudronov D., Gancarcíkova S., Vatsos I.N., Bisa S., Kiron V., Sørensen M. (2023). Supplementation of lactic acid bacteria has positive effects on the mucosal health of Atlantic salmon (Salmo salar) fed soybean meal, Aquaculture Reports, 28: 101461
Noga E.J. (1996). Fish Disease: diagnosis and treatment. St Louis: Mosby-Year Book. 367 p.
Pru?ckler M., Lorenz C., Endo A., Kraler M. Du?rrschmid K., Hendriks K., Da Silva F.S., Auterith E., Kneifel W., Michlmayr H. (2015). Comparison of homo- and heterofermentative lactic acid bacteria for implementation of fermented wheat bran in bread. Food Microbiology, 49: 211-219.
Qaddoori M.S., Najim S.M., Al-Niaeem K.S. (2022). Effects of some probiotics and synbiotic dietary supplementation on growth performance and digestive enzymes activity of common carp, Cyprinus Carpio, Journal of Pharmaceutical Negative Results, 13(7): 175-184.
Qaddoori M.S., Al-Niaeem K.S., Najim S.M. (2023). Effects of Some Dietary Additives on Growth and Health Status of the Young Common Carp Cyprinus carpio, Egyptian Journal of Aquatic Biology and Fisheries, 27(2): 221-239.
Rane M., Markad A. (2015). Effects of Probiotics on the growth and survival Zebrafish Danio rerio. International Journal of Science and Research, 4(3): 1839-1841.
Rhema Z.A., Al-Noor J.M. (2022). Health and nutritional performance of young common carp Cyprinus carpio L. feeding diets with added bakery yeast Saccharomyces cerevisiae. International Journal of Health Sciences, 6(S6): 2424-2437.
Rialita T., Sukarminah E., Yuliana T., Sumanti D.M., Kurnianingrium I., Octaviani F.N., Santoso M.B., Susanto H.F. (2019). Isolation and Identification of Lactic Acid Bacteria Producing Bio preservative Bacteriosin from Smoked Fish, IOP Conf. Series: Earth and Environmental Science, 347: 1-5.
Ringø E., Hoseinifar S.H., Ghosh K., Doan H.V., Beck B.R., Song S.K. (2018). Lactic acid bacteria in finfish-An update. Frontiers in Microbiology, 9: 1-37.
Ringø E., Van Doan H., Lee S.H., Soltani M., Hoseinifar S.H., Harikrishnan R., Song S.K. (2020). Probiotics, lactic acid bacteria and bacilli: interesting supplementation for aquaculture. Journal of Applied Microbiology, 129: 116-136.
Rombenso A., Araujo B., Li E. (2022). Recent advances in fish nutrition: Insights on the nutritional implications of modern formulations. Animals, 12(13): 1705.
Salas-Leiva J., Opazo R., Remond C., Uribe E., Velez A., Romero J. (2017). Characterization of the intestinal microbiota of wild-caught and farmed fine flounder (Paralichthys adspersus). Latin American Journal of Aquatic Research, 45(2): 370-378.
Sevier H., Raae A.J., Lied E. (2000). Growth and protein turnover in Atlantic salmon (Salmo salar): the effect of dietary protein level and protein size. Aquaculture 185: 10-20.
Shao J., Wang B., Liu M., Jiang K., Wang L., Wang M. (2019). Replacement of fishmeal by fermented soybean meal could enhance the growth performance but not significantly influence the intestinal microbiota of white shrimp Litopenaeus vannamei. Aquaculture, 504: 354-360.
Singh G., Khati A., Chauhan R. (2021). Evaluation of vitamin C as growth for promoter’s freshwater major, carp, Cyprinus carpio. Journal of Experimental Zoology Part A, 24(1): 377-382.
Soni M., Shah H.R., and Patel, S.M. (2019). Isolation, identification and analysis of probiotic characteristics of Lactobacillus spp. from Regional Yoghurts from Surendranagar District, Gujarat, Asian Journal of Dairy and Food Research, DR-1631: 1-6.
Suman G., Nupur M., Amerada S., Pradeep B. (2016). Single Cell Protein Production: A Review. Intern. International Journal of Current Microbiology and Applied Sciences, 4(9): 251-262.
Tacon A.G.J (1990). Standard methods for the nutrition and feeding of farmed fish and shrimp. In: Nutritive Sources and Composition. (2). Argent Laboratories Press, Redmond, W.A. 129 p.
Talbot C. (1985). Laboratory methods in fish feeding and nutritional studies. In: P. Tytler, P. Calow (Eds.). Fish energetics: New perspectives. Croom Helm, London and Sydney. pp: 125-154.
Taufik D., Arief M., Kenconojati H. (2019). The Effect of Different level of Probiotic Addition on Commercial Feed against Digestibility and Efficiency of Nile Tilapia Feed (Oreochromis Niloticus), IOP Conf. Series: Earth and Environmental Science, 236: 1-4.
Watts S.A., Lawrence A.L., Lawrence J.M. (2020). Nutrition. In: J.L. Lawrence (Ed.), Sea Urchins: Biology and Ecology, 4th. pp: 191-208.
Wuertz S., Schroeder A., Wanka K.M. (2021). Probiotics in fish nutrition — Long-standing household remedy or native nutraceuticals. Water, 13(10): 1348.
Xia Y., Cao J.M., Wang M., Lu M.X., Chen G., Gao F.Y. (2019). Effects of Lactococcus lactis subsp. lactis JCM5805 on colonization dynamics of gut microbiota and regulation of immunity in early ontogenetic stages of tilapia. Fish and Shellfish Immunology, 86: 53-63.
Xia Y., Wang M., Gao F., Lu M., Chen G. (2020). Effects of dietary probiotic supplementation on the growth, gut health and disease resistance of juvenile Nile tilapia (Oreochromis niloticus). Animal Nutrition, 6: 69-67.
Xiong J., Jin M., Yuan Y., Luo J.X., Lu Y., Zhou Q.C., Liang C., Tan Z.L. (2018). Dietary nucleotide-rich yeast supplementation improves growth, innate immunity and intestinal morphology of Pacific white shrimp (Litopenaeus vannamei). Aquaculture Nutrition, 24: 1425-1435.
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