Study of pollution in Shatt AL-Arab River using histological alternations and some other biochemical parameters of gill in Nile tilapia (Oreochromis niloticus) as water quality biomarkers
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This study aims to assess the pollution of Shatt AL-Arab River using histology and some other biochemical parameters of gill in Nile tilapia (Oreochromis niloticus) caught in winter 2021 at four locations as water pollution biomarkers. Some water quality parameters were determined in these sites, and the results showed that sites 2 and 3 are polluted at levels above the World Health Organization's guideline. The enzymatic and metabolism activity, histological status, and cytogenetic mutation over time in the gills were assessed. Biotransformation enzymes level showed total cytochrome p450 and Ethoxyresurofin–O– demethylase (EROD) activities are significantly increased in gills of tilapia in site 1, 2 and 3. The antioxidant enzymes activities were recorded significantly high in fish gills of catalase (CAT) and glutathione -S-transferase (GST) in polluted sites of 1, 2, and 3, while superoxide dismutase (SOD) were significantly higher only in site 3 compare with reference site of 4. The metallothionein-like protein (MTLP) was significantly higher in gills at site 3. Also, lipid peroxidation (LOP) and micronucleus analysis showed that sites 2 and 3 samples site the most affected. Gill tissue index was appeared severe alterations levels at sites 2 and 3, followed by site 1 with a relatively lower level of damage, while site 4 (reference one) showed minor or invisible changes in gill tissue. The histological alterations in the gills of Nile tilapia fish at sites 2 and 3 showed atrophy of cellular, hemorrhage, congestions, hyperplasia, and hypertrophy of the filament lamellae epithelium.
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Aebi H. (1984). Catalase in vitro. Methods In Enzymology, 105: 121-126.
Ahmad I., Oliveira, M., Pacheco M., Santos M.A., (2005). Anguilla anguilla L. oxidative stress biomarkers responses to copper exposure with or without bnaphthoflavone pre-exposure. Chemosphere, 61: 267-275.
AL-Sabti K. (1985). Chromosomal studies by blood leucocyte culture technique on three salmon IDs from Yugoslavia water. Journal of Fish Biology, 6: 5-12.
AL-Asadi S.R. (2014). Geography of Water Resources, 1st ed.; Al-Ghadeer Company for Printing and Publishing Ltd.: Basrah, Iraq. 82 p.
Al-Hejuje M.M. (2015). Application of Water Quality and Pollution Indices to Evaluate the Water and Sediments Status in the Middle Part of Shatt Al-Arab River. Ph.D. Thesis, College of Science, University of Basrah, Basrah, Iraq. 214 p.
APHA APHA/AWWA/WEF (1998). Standard methods for the examination of water and wastewater. 20th ed. APHA, Washington.
Arellano-Aguilar O., Montoya R.M., Garcia, C.M. (2009). Endogenous Functions and Expression of Cytochrome P450 Enzymes in Teleost Fish: A Review. Reviews in Fisheries Science, 17(4): 541-556.
Camargo M.M.P., Martinez, C.B.R. (2007). Histopathology of gills, kidney and liver of a Neotropical fish caged in an urban stream, Neotropical Ichthyology, 5: 327-336
Carrasco K., Tilbury K.L., and Myers M.S. (2013). Assessment of the Piscine Micronucleus Test as an in situ Biological indicator of Chemical Contaminant Effects. Canadian Journal of Fisheries and Aquatic Sciences, 47(11): 2123-2136.
Carvalho C.S., Araujo H.S.S., Fernandes M.N. (2004). Hepatic metallothionein in a teleost (Prochilodus scrofa) exposed to copper at pH 4.5 and pH 8.0. Comparative Biochemistry and Physiology Part B 137B: 225-234.
Castiglione S., Todeschini V., Franchin C., Torrigiani P., Gastaldi D., Cicatelli A., Rinaudo C., Berta G., Biondi S., Lingua G. (2009). Clonal differences in survival capacity, copper and zinc accumulation, and correlation with leaf polyamine levels in poplar: a large-scale field trial on heavily polluted soil. Environmental Pollution, 157: 2108-2117.
Cerqueira C.C., Fernandes M.N. (2002). Gill tissue recovery after copper exposure and blood parameter responses in the tropical fish Prochilodus scrofa. Ecotoxicology and Environmental Safety, 52: 83-91.
Eagderi S., Mouludi-Saleh A., Esmaeili H.R., Sayyadzadeh G., Nasri M., 2022. Freshwater lamprey and fishes of Iran; a revised and updated annotated checklist-2022. Turkish Journal of Zoology, 46: 500-522.
Fernandes M.N., Moron S.E., Sakuragui M.M. (2007). Gill morphological adjustments to environment and the gas exchange function. In: Fernandes M.N., Rantin F.T., Glass M.L., Kapoor B.G. (Eds.). Fish respiration and environment. Science Publishers, Boca Raton. pp: 93-120.
Galgani F., Payne J.F. (1991). Biological effects of contaminants: Microplate method for measurement of ethoxyresorufin-O-deethylase (EROD) in fish. Techniques in marine environmental sciences, Pala:gade 2-4, DK-1261 Copenhagen K, Denmark. pp: 1-11.
Goksøyr A., Forlin L. (1992). The cytochrome P450 system in fish, aquatic toxicology and environmental monitoring. Aquatic Toxicology, 22: 287-312.
Goss G.G., Perry S.F., Wood C.M., Laurent P. (1992). Mechanisms of ion and acid-base regulation at the gills of freshwater fish. Journal of Experimental Zoology, 263: 143-59.
Gravato C., Teles M., Oliveira M., Santos M.A. (2006). Oxidative stress, liver biotransformation and genotoxic effects induced by copper in Anguilla Anguilla L.: the influence of pre-exposure to [beta]-naphthoflavone. Chemosphere, 65: 1821-1830.
Habig W.H., Pabst M.J., Jakoby W.B. (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry 249(22): 7130- 7139.
Halliwell B., Gutteridge J.M.C. (1985). Free radicals in biology and medicine. Clarendon, UK, Oxford. 1-25.
Hughes G.M., Houlihan D.F., Rankin J.C., Shuttle-worth T.J. (1982). Gills, editors. An introduction to the study of gills. Cambridge: Cambridge Univ. Press. pp: 1-24.
Luna L.G. (1968). Manual of histologic staining methods of the Armed Forces Institute of Pathology. 3rd Edition, McGraw-Hill, New York. 86 p.
Machala M., Nezveda K., Petivalsk M., Jaroova A., Piaka V., Svobodova Z. (1997). Monooxygenase activities in carp as biochemical markers of pollution by polycyclic and polyhalogenated aromatic hydrocarbons: choice of substrates and effects of temperature, gender and capture stress. Aquatic Toxicology, 37: 113-123
Mackereth F.J.H., Heron J., Talling J.S. (1978). Water analysis: some revised methods for limnologists. vol. 36. Freshwater Biological Association Scientific Publication, Kendall. Titus Wilson. 117 p.
Morina V., Aliko V., Eldores S., Gavazaj F., Kastrati, D., Cakaj F. (2013). Histopathologic Biomarker of Fish Liver as Good Bioindicator of Water Pollution in Sitnica River, Kosovol. Global Journal of Science Frontier Research, 13(5): 1.
Ogundiran M.A., Fawole O.O., Adewoye S.O., Ayandiran T.A. (2009). Pathologic lesions in the gills of Clarias gariepinus exposed to sublethal concentrations of soap and detergent effluents. Animal Biology, 3(5): 78-82.
Ohkawa H., Ohishi N., Yagi K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95: 351-358.
Oliveira M., Ahmad I., Maria V.L., Pacheco M., Santos M.A. (2010a). A. Antioxidant responses versus DNA damage and lipid peroxidation in golden grey mullet liver: a field study at Ria de Aveiro (Portugal). Archives of Environmental Contamination and Toxicology, 59: 454-463.
Oliveira M., Ahmad I., Maria V.L., Serafim A., Bebianno J., Pacheco M., Santos M.A. (2010b). Hepatic metallothionein concentrations in the golden grey mullet (Liza aurata) relationship with environmental metal concentrations in a metal-contaminated coastal system in Portugal. Marine Environmental Research, 69: 227-233.
Pandey S., Parvez S.; Ansari R.A., Ali M., Kaur M., Hayat F., Ahmad F., Raisuddin S. (2008). Effects of exposure to multiple trace metals on biochemical, histological and ultrastructural features of gills of a freshwater fish, Channa punctata Bloch. Chemico-Biological Interactions, 174: 183-192.
Rabello-Gay M.N. (1991). Teste de micronúcleo em medula óssea. In: M.N. Rabello-Gay, M.A.L.R. Rodríguez, R. Monteleone-Neto (Eds.). Mutagênese, carcinogênese e teratogênese: métodos e critérios de avaliação. Ribeirão Preto, 1th ed. pp: 83.90
Sanchez W., Palluel O., Meunier L., Coquery M., Porcher J.M., Ait-Aissa S. (2005). Copper induced oxidative stress in three-spied stickleback: relationship with hepatic metal levels. Environmental Toxicology and Pharmacology, 19: 177-183.
Santos D.M., Melo M.R.S., Mendes D.C.S., Rocha I.K., Silva J.P., Cantanhede S.M., Meletti P.C. (2014). Histological changes in gills of two fish species as indicators of water quality in Jansen Lagoon (São Luís, Maranhão State, Brazil). International Journal of Environmental Research and Public Health, 11(12): 12927-12937.
Sayer M.D.J., Davenport J. (1987). The relative importance of the gills to ammonia and urea excretion in five seawater and one freshwater teleost species. Journal of Fish Biology, 31: 561-570.
Siraj Basha P., Usha Rani A. (2003). Cadmium-induced antioxidant defense mechanism in freshwater teleost Oreochromis mossambicus (Tilapia). Ecotoxicology and Environmental Safety 56: 218-221.
Siroka Z., Drastichová J. (2004). Biochemical marker of aquatic environment contamination - Cytochrome P450 in Fish. A Review. Acta Veterinaria Brno, 73(1): 123-132.
Storey K.B. (1996). Oxidative stress: animal adaptations in nature. Brazilian Journal of Medical and Biological Research, 29: 1715-1733.
Tavares D.C., Moura, J.F., Trejos E.A., Merico A. (2019). Traits Shared by Marine Megafauna and Their Relationships With Ecosystem Functions and Services. Frontiers in Marine Science, 6: 262.
Thompson J.A.J., Cosson R.P. (1984). An improved electrochemical method for the quantification of metallothioneins in marine organisms. Marine Environmental Research, 11: 137-152.
Van der Oost R., Beyer J., Vermeulen N.P.E. (2003). Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environmental Toxicology and Pharmacology, 13: 57-149.
Verbost P.M., Schoenmakers T.J.M., Flik G., Wendelaar-Bonga S.E. (1994). Kinetics of ATP and Na+ gradient driven Ca2+ transport in basolateral membranes from gills of freshwater and seawater adapted tilapia. The Journal of Experimental Biology, 186: 95-108.
Vinodhini R., Narayanan M. (2008). Bioaccumulation of heavy metals in organs of fresh water fish Cyprinus carpio (Common carp). International Journal of Environmental Science and Technology, 5(2): 179-182
Wael A.O., Khalid H.Z., Amr A.A., Abo-Hegab S. (2013). Risk Assessment and Toxic Effects of Metal Pollution in two Cultured and Wild Fish Species from Highly Degraded Aquatic Habitats. Archives of Environmental Contamination and Toxicology, 65(4): 753-764.
Wester P.W., Canton J.H. (1991). The usefulness of histopathology in aquatic toxicity studies. Comparative Biochemical and Physiology part C, 100(1-2): 115-117.
Whyte J.J., Jung R.E., Schmitt C.J., Tillitt D.E. (2000). Ethoxyresorufin-O-deethylase (EROD) activity in fish as a biomarker of chemical exposure. Critical Reviews in Toxicology, 30: 347-570.
Zar J.H. (1999). Biostatistical analysis, fourth ed. Publisher Upper Saddle River N.J., Prentice Hall. 663 p.
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