Isolation and identification of bacteria degrading 2,2-dichloropropionic acid in water sample

Wafaa Hassan Muslem; Hassan Muslem Abdulhussein; Mohamed Faraj Edbeib; Roswanira Abdul Wahab; Fahrul Huyop

doi:10.22034/ijab.v9i3.1269

Authors

Wafaa Hassan Muslem
wafaabio2004@yahoo.com
Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
Hassan Muslem Abdulhussein Department of Dentistry, Dijlah University College, Baghdad, Iraq.
Mohamed Faraj Edbeib Department of Animal Production, Faculty of Agriculture, Baniwalid University, Libya.
Roswanira Abdul Wahab Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia.
Fahrul Huyop Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM, Johor Bahru, Malaysia.

Vol. 9 No. 3 (2021): June

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June 24, 2021

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The widespread use of herbicides containing 2,2-dichloropropionic acid (2,2-DCP) as a recalcitrant halogen compound poses significant environmental risks and can be harmful for humen. Consequently, it is important that the bio-based detoxification method is developed in an environmental manner. This study was aimed to isolate and identify a possible degradation 2,2-DCP bacterial strain as the sole source of carbon. A bacterial dehalogenase producing 2,2-DCP was isolated from abandoned land named as WM. The WM strain was shown to have 98% sequence identity and characteristics similar to Enterobacter sp. based on 16s rRNA analysis, biochemical and morphological tests. Phylogenic analysis showed that the WM strain was Enterobacter sp. In media with 20 mM 3CP, the bacteria were well growing at 37°C, although an optimal chloride ion release was 0.48 Î¼mol Cl/mL. The first report of an Enterobacter sp. strain which can use 2, 2-DCP as its sole carbon source in a competent manner.

Muslem, W. H., Abdulhussein, H. M., Edbeib, M. F., Abdul Wahab, R., & Huyop, F. (2021). Isolation and identification of bacteria degrading 2,2-dichloropropionic acid in water sample. International Journal of Aquatic Biology, 9(3), 207–213. https://doi.org/10.22034/ijab.v9i3.1269

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