@article{Hoffmann_Duarte_Silva_2020, title={Diversity of anoxygenic phototrophic bacteria in anaerobic lagoons and facultative stabilization pond used in treatment of sewage}, volume={8}, url={https://ij-aquaticbiology.com/index.php/ijab/article/view/688}, DOI={10.22034/ijab.v8i1.688}, abstractNote={<p>The objective of this study was to identify the anoxygenic phototrophic bacteria in the anaerobic lagoons and facultative stabilization pond of the Vale do Ribeira, Cajati, Sí£o Paulo, Brazil, and their correlation with physical and chemical parameters of the ponds’ water. The samples were collected seasonally (spring, summer, autumn and winter) in the sub-surface, intermediate layer and sediment-water interface. We used the PCR/DGGE with <em>puf</em>M 557FGC and <em>puf</em>M 750R primers specific to the reaction center of the photosynthetic phototrophic bacteria for their identification. The amplification products were separated by electrophoresis on denaturing gradient gel. From the bands cut out and sequenced from DGGE, the identified bacteria were <em>Rhodopseudomonas palustris</em> (99% similarity), <em>Chromatium </em>sp<em>. </em>(92%), <em>Thiocapsa</em> sp. (90%), <em>Rhodospirillum </em>sp. (95%), <em>Roseobacter</em> sp. (93%) and other uncultured bacteria.</p>}, number={1}, journal={International Journal of Aquatic Biology}, author={Hoffmann, Nora Katia and Duarte, Iolanda Cristina Silveira and Silva, Maria Bernadete Amí¢ncio Varesche}, year={2020}, month={Feb.}, pages={9–17} }