Dietary conjugated linoleic acid (CLA) and lecithin affects levels of serum cholesterol, triglyceride, lipoprotein and hypoxic stress resistance in rainbow trout (Oncorhynchus mykiss)
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Considering the role of conjugated linoleic acid (CLA) and soybean lecithin (SBL) in fat digestion, absorption and metabolism as well as stress resistance in aquatic organisms, the current study was a 74-day attempt to investigate the synergistic effects of these two compounds on stress resistance and blood lipoprotein levels of rainbow trout (Oncorhynchus mykiss) weighing 120±5 g. Nine isonitrogenous and isocaloric experimental diets containing 1, 2 and 3% CLA and 2, 3 and 4% soybean lecithin on the basis of the basal control diet (free from CLA and lecithin) were formulated. The fish were exposed to three ascending periods of hypoxic stress (7.5, 15 and 30 min) at the end of culture period. The dissolved oxygen was decreased to 1.96 ppm by injecting nitrogen gas. Significantly higher levels of cortisol and glucose were detected in fish fed on diets having higher levels of SBL (2%) and CLA (3%) at higher stress time improving the resistance to hypoxia stress (P≤0.05). Moreover, levels of triglyceride (TRG), cholesterol (COL), low density lipoprotein (LDL), very low-density lipoprotein (VLDL) and high density lipoprotein (HDL) also increased significantly compared to the control group (P≤0.05). This study reveals that using 2% SBL and 3% CLA in rainbow trout feed can promote resistance to hypoxic stress.
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ADM Specialty Ingredients (2003). Lecithin in Aquaculture. P.O. Box 2, 1540 AA Koog aan de Zaan, Netherlands, [email protected].
AOAC (1995). Official methods of analysis of AOAC International. 2 vols. 16th edition. Arlington, VA, USA, Association of Analytical Communities.
Atar H.H., Bekcan S., Olmez, M. (2009). The effects of dietary of soybean lecithin on the growth performance feed conversion and body composition of Tilapia (Oreochromis niloticus L.) fry. Journal of Animal and Veterinary Advances, 8: 1678-1684.
Azarm H.H., Kenari A.A., Hedayati M. (2012). Effect of dietary phospholipid sources and levels on growth performance, enzymes activity, cholecystokinin and lipoprotein fractions of rainbow trout (Oncorhynchus mykiss). Aquaculture Research, 1-11.
Barton B.A., Iwama G.K. (1991). Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annual Review of Fish Diseases, 1: 3-26.
Berge G.M., Ruyter B., Asgard T. (2004). Conjugated linoleic acid in diets for juvenile Atlantic salmon (Salmo salar); Effects on fish performance, proximate composition, fatty acid and mineral content. Aquaculture, 237: 365-380.
Bloom J.H., Ebeling J.M., Dabrowski K. (1993). Engineering design and performance of a small scale packed bed oxygenation and degassing column for a laboratory holding facility. In: J.K. Wang (Ed.), Techniques for Modern Aquaculture. Proceeding Aquaculture Engineering Conference, Spokane, WA. pp: 506-510.
Burel C., Boujard T., Kaushik S.J., Boeuf G., Van Der Geyten S., Mol K.A., Kuhn E.R., Quinsac A., Krouti M., Ribailler D. (2001). Effects of rapeseed meal-glucosinolates on thyroid. General and Comparative Endocrinology, 124(3): 343-358.
Choi B.D., Kang S.J., Ha Y.L., Ackman R.G. (1999). Accumulation of Conjugated Linoleic Acid (CLA) in Tissues of Fish Fed Diets Containing Various Levels of CLA. In: Y.L. Xiong, C.T. Ho, F. Shahidi (Eds.). Quality Attributes of Muscle Foods. Kluwer Academic/Plenum Publishers, New York. pp: 61-71.
Dabrowskia K., Leea K.J., Guzb L., Verlhacc V., Gabaudan J. (2004). Effects of dietary ascorbic acid on oxygen stress (hypoxia or hyperoxia), growth and tissue vitamin concentrations in juvenile rainbow trout (Oncorhynchus mykiss). Aquaculture, 233: 383-392.
FAO. (2014). Food and Agriculture Organization of the United Nations. The State of World Fisheries and Aquaculture, 2-6.
Fernandez-Palacios H. (2002). Necessity of dietary lecithin and eicosapentaenoic acid for growth, survival, stress resistance and lipoprotein formation in gilthead sea bream Sparus aurata. Fisheries Science, 68: 1165-1172.
Fontagne S., Burtaire L., Corraze G., Bergot P. (2000). Effects of medium change chain triacylglycerols (tricaprylin and tricaproin) and phospholipid supply on survival, growth and lipid metabolism in common carp (Cyprinus carpio L.) larvae. Aquaculture, 190: 289-303.
Fontagne S., Geurden I., Escaffre A.M., Bergot P. (1998). Histological changes induced by dietary phospholipids in intestine and liver of common carp (Cyprinus carpio L.) larvae. Aquaculture, 161: 213-223.
Fukuda H. (1985). On the blood sugar of freshwater fish. Bulletin of the Japanese Society for the Science of Fish, 23: 782-784.
Hadas E., Koven W., Sklan D., Tandler A. (2003). The effect of dietary phosphatidylcholine on the assimilation and distribution of ingested free oleic acid (18:1n-9) in gilthead sea bream (Sparus aurata) larvae. Aquaculture, 217: 577-588.
Hung S. (1989). Choline requirement of hatchery-produced juvenile white sturgeon (Acipenser transmontanus). Aquacalture,78: 183-194.
Hung S.S.O., Storebakken T., Cui Y., Tian L., Einen O. (1997). High energy diets for white sturgeon (Acipenser transmontanus). Aquaculture Nutrition, 3: 281-186.
Hung S.S.O., Lutes P.B. (1988). A preliminary study on the nonessentiality of lecithin for hatchery produced juvenile white sturgeon (Acipenser transmontanus). Aquaculture, 68: 353-360.
Kanazawa A. (1979). Effect of docosahexanoic acid and phospholipids on stress tolerance of fish. Aquaculture, 155(1): 129-134.
Kennedy S.R., Campbell P.J., Porter A., Tocher D.R. (2005). Influence of dietary conjugated linoleic acid (CLA) on lipid and fatty acid composition in liver and flesh of Atlantic salmon (Salmo salar). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 141(2): 168-178.
Ketola H.G. (1976). Choline metabolism and nutritional requirement of lake Trout, Salvelinus nanaycush. Journal of Animal Science, 43: 474-477.
Wahle K.W.J.W, Goua M., D'Urso S., Heys S.D. (2008). Conjugated linoleic acid effects on body composition and clinical biomarkers of disease in animals and man: metabolic and cell mechanisms. In: A.J. Dijkstra, R.J. Hamilton, w. Hamm. Trans Fatty Acids, Blackwell Publishing, UK. pp: 54-101
Leaver M.J., Tocher D.R., Obach A., Jensen L., Henderson R.J., Porter A.R., Krey G. (2006). Effect of dietary Conjugated Linoleic Acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo Salar) tissues. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 145(2): 258-267.
Liu J., Caballero M.J., Izquierdo M., Ali E-S.T., Hernandez-Cruz M., Valencia A., Fernandez-Palacios H. (2002). Necessity of dietary lecithin and eicosapentaenoic acid for growth, survival, stress resistance and lipoprotein formation in gilthead sea bream Sparus aurata. Fisheries Science, 68: 1165-1172.
Luo Z., Tan X.Y., Liu C.X., Li X.D., Liu X.J., Xi W.Q. (2011). Effect of dietary conjugated linoleic acid levels on growth performance, muscle fatty acid profile, hepatic intermediary metabolism and antioxidant responses in genetically improved farmed Tilapia strain of Nile tilapia Oreochromis niloticus. Aquaculture Research, 1392-1403.
Makol A., Torrecillas S., Fernández-Vaquero A., Robaina L., Montero D., Caballero MJ., Tort L, Izquierdo M. (2009). Effect of conjugated linoleic acid on dietary lipids utilization, liver morphology and selected immune parameters in sea bass juveniles (Dicentrarchus labrax). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 154(2): 179-187.
Maleki Moghaddam M.R. (2015). Nutritional strategies affect heat increment and ammonia production rate in intensive fish farms. International. Journal of Fisheries and Aquatic Studies, 3(2): 431-436.
Martinez-Porchasi M., Martinez-Cordova L.R., Ramos-Enriquez R. (2009). Cortisol and Glucose: Reliable indicators of fish stress? American Journal of Aquatic Sciences, 4(2): 158-178.
McDonald P., Greenhalgh J.F.D., Morgan C.A., Sinclair L.A. (1979). Animal Nutrition. In: L.M. maynard. McGraw-Hill Publications. pp: 207-214.
NRC. (2011). Nutrient requirements of fish and shrimp. National Academies Press. 392 p.
Olsen R.E., Myklebust R., Kaino T., Ringo E. (1999). Lipid digestibility and ultrastructural changes in the enterocytes of Arctic char (Salvelinus alpines L.) fed linseed oil and soybean lecithin. Fish Physiology and Biochemistry, 21: 35-44.
Pariza M.W., Park Y., Cook M.E. (2001). The biologically active isomers of conjugated linoleic acid. Progress in Lipid Research, 40(4): 283-298.
Poston H.A. (1991). Response of Atlantic salmon fry to feed prude to lecithin and choline. Progressive Fish Culturist, 53: 224-228.
Pickering A.D., Pottinger T.G. (1989). Stress responses and disease resistance in salmonid fish: effects of chronic elevation of plasma cortisol. Fish Physiology and Biochemistry, 7: 253-258.
Pierson P.M., Lamers A., Flik G., Mayer-Gostan N. (2004). The stress axis, stanniocalcin, and ion balance in rainbow trout. General and Comparative Endocrinology, 137: 263-271.
Ramsay J.M., Feist G.W., Varga Z.M., Westerfield M., Kent M.L., Schreck C.B. (2006). Whole-body cortisol is an indicator of crowding stress in adult zebrafish (Danio rerio). Aquaculture, 258: 565-574.
Rohmah Z., Fitria L., Kang S.J., Kim J.S., Lee J.J., Ha Y.L., Choi B.D. (2016). Anti-obesity effects of Lipid Extract from Sea-reared of Rainbow Trout (Oncorhynchus mykiss) Fed with Sea Squirt (Halocynthia roretzi) Tunic's Carotenoids and CLA. Journal of Nutrition and Food Sciences, 6(4): 525- 532.
Salhi M., Hernandez-Cruz C.M., Bessonart M., Izquierdo M.S., Fernandez-Palacios H. (1999). Effect of different dietary polar lipid levels and different n-3 HUFA content in polar lipids on gut and liver histological structure of gilthead seabream (Sparus aurata). Aquaculture, 179(1-4): 253-263.
Sanders S.R., Teachey M.K., Ptock A., Kraemer K., Hasselwander O., Henriksen E.J., Baumgard L.H. (2004). Effects of specific conjugated linoleic acid isomers on growth characteristics in obese Zucker rats. Lipids, 39(6): 537-543.
SAS Institute. (2008). SAS Stat User's Guide. Version 9.1 ed. SAS Inst. Inc.
Schreck C.B., Contreras-Sanchez W., Fitzpatrick M.S. (2001). Effects of stress on fish reproduction, gamete quality, and progeny. Aquaculture, 197: 3-24.
Tago A., Yamamato Y., Teshima S., Kanazawa A. (1999). Effects of 1,2-di-20:5 phosphatidylcholine (PC) & 1,2-di-22:6-PC on growth and stress tolerance of Japanese flounder (Paralichthys olivaceus) larvae. Aquaculture, 179, 1-4.
Tocher D.R., Eldar A., Bendiksen P.J., Campbell J., Gordon B. (2008). The role of phospholipids in nutrition and metabolism of teleost fish. Aquaculture Journal, 280: 21-34.
Twibell R.G., Wilson R.P. (2003). Effects of conjugated linoleic acids and total lipid concentrations on growth responses of juvenile channel catfish, Ictalurus punctatus. Aquaculture, 221: 621-628.
Valente L.M.P., Bandarra N.M., Figueiredo-Silva A.C., Rema P., Vaz-Pires P., Martins S., Prates J.A.M., Nunes M.L. (2005). Conjugated linoleic acid in diets for large-size rainbow trout (Oncorhynchus mykiss): Effects on growth, chemical composition and sensory attributes. British Journal of Nutrition, 97: 289-297.
Wendelaar-Bonga S.A. (1997). The stress response in fish. Physiological Reviews, 77: 591-625.
Yang C.G., Wen H., Jiang M., Liu W., Wu F., Tian J., Wei Q.W. (2014). Effect of dietary conjugated linoleic acid supplementation on the growth, lipid metabolism, and plasma lipid levels of juvenile Amur sturgeon (Acipenser schrenckii Brandt, 1869). Journal of Applied Ichthyology, 30(6): 1626-1631.
Zanqui A.B., Maruyama S.A., Barilli D.J., Ribeiro S.A.O., Gomes S.T.M., Visentainer J.V., Souza N.E., Boscolo W.R., Matsushita M. (2013). Incorporation of conjugated linoleic and alpha linolenic fatty acids into Pimedolus maculatus fillets. Food Science and Technology (Campinas), 33(3): 532-540.
Zhao Z., Wu T., Tang H., Zhang J. (2008). Influence of dietary conjugated linoleic acid on growth, fatty acid composition and hepatic lipogenesis in large yellow croaker (Pseudosciaena crocea R.). Journal of Journal Zhejiang University Science B., 9(9): 691-700.
Zuo R., Ai Q., Mai K., Xu W. (2013). Effects of conjugated linoleic acid on growth, non-specific immunity, antioxidant capacity, lipid deposition and related gene expression in juvenile large yellow croaker (Larmichthys crocea) fed soyabean oil-based diets. British Journal of Nutrition, 110(7): 1220-1232.
