Study of fungicidal properties of colloidal silver nanoparticles (AgNPs) on trout egg pathogen, Saprolegnia sp.

https://doi.org/10.22034/ijab.v3i3.97
Antifungal In vitro Saprolegnia Silver nanoparticles Rainbow trout.

Authors

  • Seyed Ali Johari
    [email protected]
    Fisheries Department, Faculty of Natural Resources, University of Kurdistan, ZIP Code: 66177-15175, P.O. Box 416, Sanandaj, Kurdistan, Iran.
  • Mohammad Reza Kalbassi Department of Aquaculture, Marine Science Faculty, Tarbiat Modares University, Mazandaran, Noor, Iran.
  • Mehdi Soltani Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Il Je Yu Institute of Nanoproduct Safety Research, Hoseo University, Asan, Korea.
Vol. 3 No. 3 (2015): June
Articles
May 22, 2015

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Silver nanoparticles (AgNPs) are known to have bactericidal and fungicidal effects. Since, there is few information available on the interaction of colloidal nanosilver with fish pathogens. Hence, the current study investigated the effects of colloidal AgNPs on the in vitro growth of the fish pathogen Saprolegnia sp.. Before the experiments, various important properties of AgNPs were well-characterized. The antifungal activity of AgNPs was then evaluated by determining the minimum inhibitory concentrations (MICs) using two-fold serial dilutions of colloidal nanosilver in a glucose yeast extract agar at 22ºC. The growth of Saprolegnia sp. on the AgNPs agar treatments was compared to that of nanosilver-free agar as controls. The results showed that AgNPs have an inhibitory effect on the in vitro growth of the tested fungi. The MIC of AgNPs for Saprolegnia sp. was calculated at 1800 mg/L, which is equal to 0.18 percent. It seems that AgNPs could be a proper replacement for teratogenic and toxic agents, such as malachite green. In addition, the indirect use of AgNPs could be a useful method for providing new antifungal activity in aquaculture systems.