Methylation levels of lysozyme gene in rainbow trout (Oncorhynchus mykiss) fed by commercial immunogen probiotic
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In the present study, we evaluated the effect of commercial immunogenic prebiotic on the rate of methylation of type-C gene. A total of 120 rainbow trout were divided into two treatments, including a control group and another one with 2.0% commercial prebiotic immunogen each in three replicates. On the first, 15 and 45 days, DNA of adrenal tissue was extracted and treated with bisulfite. Samples were amplified by polymerase chain reaction (PCR) and sequenced. Based on the results, there was no significant difference (P<0.05) between the 1st day (R), 15th day of control (C), 15th day of immunogen (I) and 40th day of control (CS). However, there was a significant difference (P<0.05) between the 45th day immunogenicity (IS) and other samples, i.e. the CpG islet methylation rate in the IS samples was lower leading to increase in the expression of the lysozyme gene
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Anastasiadi D., Díaz N., Piferrer F. (2017) Small ocean temperature increases elicit stage-dependent changes in DNA methylation and gene expression in a fish, the European sea bass. Scientific Reports, 7: 12401.
Asselman J., De Coninck D.I.M., Vandegehuchte M.B., Jansen M., Decaestecker E., De Meester L., Vanden Bussche J., Vanhaecke L., Janssen C.R., De Schamphelaere K.A.C. (2015). Global cytosine methylation in Daphnia magna depends on genotype, environment, and their interaction. Environmental Toxicology and Chemistry, 34: 1056-1061.
Austin B., Austin D.A. (2016). Bacterial fish pathogens: Disease of farmed and wild fish, sixth edition. Springer International Publishing. 552 p.
Blouin M.S., Thuillier V., Cooper B., Amarasinghe V., Cluzel L., Araki H., Grunau C. (2010) No evidence for large differences in genomic methylation between wild and hatchery steelhead (Oncorhynchus mykiss). Canadian Journal of Fisheries and Aquatic Sciences, 67: 217-224.
Fan X., Hou T., Sun T., Zhu L., Zhang S., Tang K., Wang Z. (2019) Starvation stress affects the maternal development and larval fitness in zebrafish (Danio rerio). Science of the Total Environment, 695: 133897.
Fernandez-Fernandez A., Esteller M., Walter J. (2007). DNA Methylation Analysis by Bisulfite Sequencing (BS) (PROT34). Methods in Molecular Biology, 791: 11-21.
Fooks L.J., Gibson G.R. (2002). Probiotics as modulators of the gut flora. British Journal of Nutrition, 88: s39-s49.
Herceg Z. (2007). Epigenetics and cancer: Towards an evaluation of the impact of environmental and dietary factors. Mutagenesis, 22: 91-103.
Huynh T.G., Shiu Y.L., Nguyen T.P., Truong Q-P., Chen J-C., Liu C-H. (2017). Current applications, selection, and possible mechanisms of actions of synbiotics in improving the growth and health status in aquaculture: A review. Fish and Shellfish Immunology, 64: 367-382
Khurana S.K. (2005). Effect of probiotics supplementation on immuno-competence and in prevention of experimental Salmonella gallinarum infection in broiler chicken. Proc 15th Eur Symp Poult Nutr Balatonfí¼red, Hungary, 25-29 Sept 2005. pp: 283-285.
Lee H-S. (2015). Impact of maternal diet on the epigenome during in utero life and the developmental programming of diseases in childhood and adulthood. Nutrients, 7: 9492-9507.
Lętowska-Andrzejewicz K., Torres A., Torres K., et al (2011). The use of morphometric and fractal parameters to assess the effects of 5-fluorouracil, interferon and dexamethasone treatment on colonic anastomosis healing: an experimental study in rats. Folia Histochemica et Cytobiologica, 49: 80-809.
Li Y., Tollefsbol T.O. (2011). DNA methylation detection: Bisulfite genomic sequencing analysis. Methods in Molecular Biology, 791: 11-21.
Liu C.H., Chiu C.H., Wang S.W., Cheng W. (2012.) Dietary administration of the probiotic, Bacillus subtilis E20, enhances the growth, innate immune responses, and disease resistance of the grouper, Epinephelus coioides. Fish and Shellfish Immunology, 33: 699-706.
Hashim M.M. (2016). study of the yeast cell wall components, mannan oligosaccharide and beta glucan, to determine their individual and synergistic influence on broiler performance under pathologic stress a dissertation. Texas A&M University.100 p.
Morán P., Pérez-Figueroa A. (2011). Methylation changes associated with early maturation stages in the Atlantic salmon. BMC Genetics, 12(86): 12-86.
Novik KL, Nimmrich I, Genc B, et al (2002). Epigenomics: Genome-Wide Study of Methylation Phenomena 111 Epigenomics: Genome-Wide Study of Methylation Phenomena.
Portela A., Esteller M. (2010). Epigenetic modifications and human disease. Nature Biotechnology, 28: 1057-1068.
Sado R.Y., Bicudo í.J.D.A., Cyrino J.E.P. (2008). Feeding Dietary mannan oligosaccharides to juvenile nile tilapia, Oreochromis niloticus , Has no effect on hematological parameters and showed decreased feed consumption. The Journal of the World Aquaculture Society, 39: 821-826.
Salze G., McLean E., Schwarz M.H., Craig S.R. (2008). Dietary mannan oligosaccharide enhances salinity tolerance and gut development of larval cobia. Aquaculture, 274: 148-152.
Tierling S., Reither S., Walter J. (2007). Bisulfite sequencing of small DNA/cell samples. Methods in Molecular Biology, 889: 385-406.
Waterland R.A., Michels K.B. (2007). Epigenetic Epidemiology of the Developmental Origins Hypothesis. Annual Review of Nutrition, 27: 363-388.
Xiao J., Song C., Liu S., Tao M., Hu J., Wang J., Liu W.,
Zeng M., Liu Y. (2013). DNA methylation analysis of allotetraploid hybrids of red crucian carp (Carassius auratus red var.) and Common Carp (Cyprinus carpio L.). PLoS One, 8: e56409.
Zargar S.J., Rabbani A. (2002) Interaction of daunomycin antibiotic with histone H1: Ultraviolet spectroscopy and equilibrium dialysis studies. International Journal of Biological Macromolecules, 30: 113-117.
Zargar S.J., Rabbani A. (2000). The effects of daunomycin antibiotic on histone H1: Thermal denaturation and fluorescence spectroscopy studies. International Journal of Biological Macromolecules, 28: 75-79.
Zheng J., Xiao X., Zhang Q., Yu M. (2014). DNA methylation: The pivotal interaction between early-life nutrition and glucose metabolism in later life. British Journal of Nutrition, 112: 1850-1857.
