Toxic effects of Cadmium on antioxidant defense systems and lipid peroxidation in Acipenser persicus (Borodin, 1897)

Roghieh Safari

Abstract

Cadmium is considered as a common residue in water and sediments which could readily enter aquatic organisms. The aim of this study was to evaluate the time- and concentration-dependent changes in antioxidant enzymes (SOD and CAT) activities as well as concentration of MDA as a bi- product of lipid peroxidation in the liver of Persian sturgeon, Acipenser persicus, following CdCl2 exposure at sub-lethal concentrations for 14 days. Based on the results, activity of SOD and CAT showed a significant increase in the fish exposed to different concentrations of CdCl2 up to the day 7, and then their activity decreased in the fish of all treatments on the 14th day. In all treatments, MDA content significantly increased after exposure at first day until the end of the experiment. The levels of SOD, CAT and MDA followed a concentration-dependent manner and its increase was higher in 800 µgl-1 than those of 200 µgl-1. The results suggested that antioxidant enzymes could be used as an effective index to monitor ecotoxicological changes.

Keywords

Oxidative stress, Superoxide dismutase Catalase, Malondialdehyde, Persian sturgeon.

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References

Aebi H. (1984). Catalase in vitro. Method enzymology, 105: 121-126.

Almeida J.A., Barreto R.E., Novelli E.L.B., Castro F.J., Moron S.E. (2009). Oxidative stress biomarkers and aggressive behavior in fish exposed to aquatic cadmium contamination. Neotropical ichthyology, 7(1): 103-108.

Asagba S.A., Eriyamremu G.E., Igberaese A. (2008). Bioaccumulation of cadmium and its biochemical effect on selected tissues of the catfish (Clarias gariepinus). Fish Physiology and Biochemistry, 34: 61-69.

Ballesteros M.L., Wunderlin D.A., Biston M.A. (2009). Oxidative stress responses in different organs of Ienynsia multidentata exposed to endosulfan. Ecotoxicology and Environmental Safety, 72: 199-205.

Basha P.S., Rani A.U. (2003). Cadmium-induced antioxidant defense mechanism in freshwater teleost Oreochromis mossambicus (Tilapia). Ecotoxicology and Environmental Safty, 56(2): 218-221.

Banaee M., Mehrpak M., Nematdoost Haghi B., Noori A. (2015). Amelioration of cadmium-induced changes in biochemical parameters of the muscle of Common Carp (Cyprinus carpio) by Vitamin C and Chitosan. International Journal of Aquatic Biology, 2(6): 362-371.

Belyaeva E.A., Sokolova T.V., Emelyanova L.V., Zakharova I.O. (2012). Mitochondrial electron transport chain in heavy metal-induced neurotoxicity: effects of cadmium, mercury, and copper. The Scientific World Journal, 1-14.

Dabas A., Nagpure N.S., Kumar R., Kushwaha B., Kumar P., Lakra W.S. (2012). Assessment of tissue-specific effect of cadmium on antioxidant defense system and lipid peroxidation in freshwater murrel (Channa punctatus). Fish Physiology and Biochemistry, 38: 469-482.

Dazy M., Masfaraud J.F., Ferard J.F. (2009). Induction of oxidative stress biomarkers associated with heavy metal stress in Fontinalis antipyretica Hedw. Chemosphere, 75: 297-302.

Dhindsa R.S., Plumb-Dhindsa P., Thorpe T.A. (1981). Leaf sequence correlated with increased levels of membrane permeability and lipid peroxidation and decrease levels of superoxide dismutase and catalase. Journal of Exprimental Botany, 32: 93-101.

Dorts J., Silvestre F., Tu H.T., Tyberghein A.E., Phuong N.T., Kestemont P. (2009). Oxidative stress protein carbonilation and heat shock proteins in the black tiger shrimp (Penaeus monodon) following exposure to endosulfan and deltamethrin. Environmental Toxicology and Pharmacology, 28(2): 302-310.

Duribe J., Ogwuegbe M., Egwurugwu J. (2007). Heavy metal pollution and human biotoxic effects. International Journal of Physical Sciences, 2: 112-118.

Ercal N., Gurer-Orhan H., Aykan-Burns N. (2001). Toxic metals and oxidative stress. Part I: Mechanisms involved in metal induced oxidative damage. Current Topics in Medicinal Chemistry, 1: 529-539.

Fatima M., Ahmad I., Sayeed I., Athar M., Raissuddin S. (2000). Pollutant inducedover-activation of phagocytes is concomitantly associated with peroxide damage in fish tissues. Aquatic Toxicology, 49: 243-250.

Filek M., Keskinen R., Hartikainen H., Szarejko J., Jaanniak A., Miszalski Z., Golda A. (2008). The protective role of selenium in rape seedlings subjected to cadmium stress. Plant Physiology, 165: 833-844.

Ghazie A., Al-Sawasfi A., Yan Y. (2013). Alternation of Acetylcholinesterase activity and antioxidant capacity of zebrafish brain and muscle exposed to sublethal level of cadmium. International Journal of Environmental Sciences and Development, 4(3): 327-330.

Garcia Sampaio F.G., Boijink C.L., Oba E.T., Santos L.R.B., Kalinin A.L., Rantin F.T. (2008). Antioxidant defenses and biochemical changes in pacu (Piaractus mesopotamicus) in response to single and combined copper and hypoxia exposure. Comparative Biochemistry and Physiology, 147: 43-51.

Haung H.Q., Xiao Z.Q., Lin Q.M., Chen P. (2005). Characteristics of trapping various organophosph-orous pestisides with a ferritin reactor of shark liver (Sphyrna zygaena). Analalitical Chemistry, 77: 1920-1927.

Jia X., Zhang H., Liu X. (2011). Low levels of cadmium exposure induce DNA damage and oxidative stress in liver of Oujiang colored carp (Cyprinus carpio) var color. Fish physiology and biochemistry, 37: 97-103.

Kaplin P.A. (1995). Implication of climate change and water level rise in the Caspian Sea region. United Nation Environment Programme.

Kim S.G., Park D.K. Jange S.W., Lee J.S., Kim S.S., Chung M.H. (2008). Effects of dietary benzo[a]pyrene on growth and hematological parameters in juvenile rockfish, Sabates schlegeli (Hilgendrof). Bulletin of Environmental Contamination and Toxicology, 81: 470-474.

Lackner R. (1998). Oxidative stress in fish by environmental pollutants. In: T. Braunbeck, D.E. Hinton, B. Sterite (Eds.). Fish Ecotoxicology. Bickbaurser, Basel. pp. 203-224.

Lenhardt M., Jaric I., Kalauzi A., Cvijanovic G. (2006). Assessment of extinction risk and reasons for decline in sturgeon. Biodiversity and Conservation, 15: 1967-1976.

Livingstone D.R. (2003). Oxidative stress in aquatic organism in relation to pollution and aquaculture. Revue De Medecine Veterinaire, 154: 427-430.

Londis W.G., Yu M.H. (1995). Introduction to environmental toxicology: impacts of chemical upon ecological system. Lewis Publication, Boca Raton.

Lowery O., Rosebrough N., Farr N., Randall R. (1951). Protein measurement with Folin phenol reagent. Journal of Biological Chemistry. 193: 265-275.

Madesto K.A., Martinez C.B.R. (2010). Roundup causes oxidative stress in the liver and inhibits actylcholinesterase in muscles and the brain of the fish Prochilodus lineatus. Chemosphere, 78: 294-299.

Mani R., Meena B., Valivittan K., Suresh A. (2014). Glutathione- S- Transferase and Catalase activity in different tissues of marine Catfish (Arius arius) on exposure to Cadmium. International Journal of Pharmacy and Pharmaceutical Sciences, 6(1): 326-332.

Mirzaee M., Nezami S., Mehdinejad K. (2003). The toxicity of heavy metals on Persian sturgeon. MS.c. Thesis. Islamic Azad University, Lahijan Branch. 100 p.

Miyamoto S., Dupas C., Murota K., Terao J. (2003). Phospholipid hydroperoxides are detoxified by phospholipase A2 and GSH peroxidase in rat gastric mucosa. Lipids, 38(6): 641-649.

Oner M., Atli G., Canli M. (2008). Changes in serum biochemical parameters of freshwater fish Oreochromis niloticus following prolonged metal (Ag, Cd, Cr, Cu, Zn) exposures. Environmental Toxicology and Chemistry, 27(2): 306-366.

Pandey S., Parvaz S., Sayeed I., Haque R., Bin-Hafeez B., Raisuddin S. (2003). Biomarker of oxidative stress: a comparative study of river Yamuna fish Wallage attu. Science of the Total Environment, 309: 105-115.

Pourang N., Tanabe S., Rezvani S., Dennis H. (2005). Trace elements accumulation in edible tissues of five sturgeon species from the Caspian Sea. Environmental Monitoring and Assessment, 100: 89-108.

Reynder H., Campenhout K.W., Bervoet L., Coen W.M.D., Blust R. (2006). Dynamics of cadmium accumulation and effects in common carp (Cyprinus carpio) during simultaneous exposure to water and food (Tubifex tubifex). Environmental Toxicology and Chemistry, 25(6): 1558-1567.

Romeo M., Benni N., Gnassia-Barelli M., Lafaurie M., Girard J. (2000). Cadmium and copper display different responses toward oxidative stress in the kidney off the sea bass Dicentrarchus labrax. Aquatic Toxicology, 48: 185-194.

Rastgoo L., Alemzade A. (2011). Biochemical responses of Gouan (Aeluropus littoralis) to heavy metals stress. Australian Journal of Crop Science, 5(4): 375-383.

Safari R., Shabani A., Ramezanpour S., Imanpour M.R. (2014). Alternations of heat shock proteins (hsp70) gene expression in Persian sturgeon (Acipenser persicus) exposed to cadmium chloride. Iranian Journal of Fisheries Science, 13(4): 979-997.

Saglam D., Atli G., Dogan Z., Baysoy E., Gurler C., Eroglu A., Canli M. (2014). Response of the antioxidant system of freshwater fish (Oreochromis niloticus) exposed to metals (Cd, Cu) IN DIFFERING HARDNESS. Turkish Journal of Fisheries and Aquatic Sciences, 14: 43-52.

Scott G.R., Sloman K.A. (2004). The effects of environmental pollutants on complex fish behaviour: integrating behavioural and physiological indicators of toxicity. Aquatic Toxicology, 68: 369-392

Sevcikova M., Modra H., Slaninova A., Svobodova Z. (2011). Metals as a cause of oxidative stress in fish. A review. Veterinary Medicine, 56: 537-546.

Shariati F., Esmaili Sari A., Mahinchian A., Pourkazemi M. (2011). Metallothionein as potential biomarker of cadmium exposure in Persian Sturgeon (Acipenser persicus). Biological Trace Element Research, 143: 281-291.

Sharma D.K., Ansari B.A. (2014). Toxicity of azadirachtin on some biomarkers of oxidative stress in zebra fish, Danio rerio. Journal of Biology and Earth Science, 4(2): 160-167.

Sharma D.K., Ansari B.A. (2013). Effect of deltamethrin CAT, LPO and GSH in tissue of zebra fish, Danio rerio. Research Journal of Environmental Toxicity, 7: 38-46.

Stohs S.J., Hagchi D., Hassoun E., Bagchi M. (2000). Oxidative mechanisms in the toxicity of chromium and cadmium ions. Journal of Environmental Pathology Toxicology and Oncology, 19: 201-213.

Wang W., Batterman S., Chernyak S., Nriagu A. (2008). Concentrations and risks of organic and metal contaminants in Eurasian caviar. Ecotoxicology Environmental Safety, 71(1): 138-148.

Zahedi S., Mirvaghefi A.R., Rafati M., Mehrpoosh M. (2013). Cadmium accumulation and biochemical parameters in juvenile Persian sturgeon, Acipenser persicus, upon sublethal cadmium exposure. Comparative Clinical Pathology, 22: 805-813.

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