Diversity of anoxygenic phototrophic bacteria in anaerobic lagoons and facultative stabilization pond used in treatment of sewage

Nora Katia Hoffmann; Iolanda Cristina Silveira Duarte; Maria Bernadete Amí¢ncio Varesche Silva

doi:10.22034/ijab.v8i1.688

Authors

Nora Katia Hoffmann
kasaavedra@ufg.br
School of Civil and Environmental Engineering of the Federal University of Goiás, Brazil, (EECA-UFG)
Iolanda Cristina Silveira Duarte Department of Biology, Federal University of Sào Carlos - UFSCar, Joào Leme dos Santos Highway, Km 101, CEP 18052-780, Sorocaba, Sào Paulo, Brazil.
Maria Bernadete Amí¢ncio Varesche Silva Laboratory of Biological Processes, Department of Hydraulics and Sanitation, Engineering School of Sào Carlos – University of Sào Paulo (EESC – USP) Campus II, Sào Carlos, SP, CEP 13563-120, Brazil.

Vol. 8 No. 1 (2020): February

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February 23, 2020

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The objective of this study was to identify the anoxygenic phototrophic bacteria in the anaerobic lagoons and facultative stabilization pond of the Vale do Ribeira, Cajati, Sí£o Paulo, Brazil, and their correlation with physical and chemical parameters of the ponds' water. The samples were collected seasonally (spring, summer, autumn and winter) in the sub-surface, intermediate layer and sediment-water interface. We used the PCR/DGGE with pufM 557FGC and pufM 750R primers specific to the reaction center of the photosynthetic phototrophic bacteria for their identification. The amplification products were separated by electrophoresis on denaturing gradient gel. From the bands cut out and sequenced from DGGE, the identified bacteria were Rhodopseudomonas palustris (99% similarity), Chromatium sp. (92%), Thiocapsa sp. (90%), Rhodospirillum sp. (95%), Roseobacter sp. (93%) and other uncultured bacteria.

Hoffmann, N. K., Duarte, I. C. S., & Silva, M. B. A. V. (2020). Diversity of anoxygenic phototrophic bacteria in anaerobic lagoons and facultative stabilization pond used in treatment of sewage. International Journal of Aquatic Biology, 8(1), 9–17. https://doi.org/10.22034/ijab.v8i1.688

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Achenbach L.A., Carey J, Madigan M.T. (2001). Photosynthetic and phylogenetic primers for detection of anoxygenic phototrophs in natural environments. Applied and Environmental Microbiology, 67: 2922-2926.

Andrade Neto C.O. (1997). Sistemas simples para tratamento de esgotos sanitários – Experiíªncia Brasileira. Rio de Janeiro – ABES. v.2. 301 p.

APHA, AWWA, WPCF (2012). Standard methods for the examination of water and wastewater. 20 ed. Washington, D.C. 850 p.

Beja O., Suzuki M., Heidelberg J. (2002). Unsuspected diversity among marine aerobic anoxygenic phototrophs. Letters to Nature, 415: 630-633.

Belila A., Ghrabi A., Hassen A. (2011). Molecular analysis of the spatial distribution of sulfate-reducing bacteria in three eutrophicated wastewater stabilization ponds. Annals of Microbiology, 61: 563-573.

Belila A., Fazaa I., Hassen A., Ghrabi A. (2013a). Anoxygenic phototrophic bacterial diversity within wastewater stabilization plant during "red water" phenomenon. International Journal of Environmental Science and Technology, 10: 837-846.

Belila A., Abbas B., Fazaa I., Saidi N., Snoussi M., Hassen A., Muyzer G. (2013b). Sulfur bacteria in wastewater stabilization ponds periodically affected by the "˜red-water" phenomenon. Applied Microbiology and Biotechnology, 97: 379-394.

Canhos V.P., Vazoller R.F. (1999). Biodiversidade do Estado de Sí£o Paulo: Microrganismos e vírus. 1 ed. Sí£o Paulo: FAPESP. 150 p.

Esteves F.A. (1998). Fundamentos de limnologia. 2ed. Rio de Janeiro, Interciíªncia. 602 p.

Griffiths R.I., Whiteley A.S., O'donnell A.G., Bailey M.J. (2000). Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA and rRNA-based microbial community composition. Applied and Environmental Microbiology, 66: 5488-5491.

Hiraishi A., Ueda Y. (1995). Isolation and characterization of Rhodovulum strictum sp. nov. and some other purple nonsulfur bacteria from colored blooms in Tidal and seawater pools. International Journal of Systematic Bacteriology, 45: 319-326.

Hulsen T., Batstone D.J., Keller J. (2014). Phototrophic bacteria for nutrient recovery from domestic wastewater. Water Research, 50: 18-26.

Karr E., Sattley W.M., Jung D.O., Madigan M.T., Achenbach L. (2003). Remarkable diversity of phototrophic purple bacteria in a permanently frozen Antarctic lake. Applied and Environmental Microbiology, 69: 4910-4914.

Kim M., Choi K-M., Yin Ch-R., Lee K-Y., Im W-T., Lim H.H., Lee S-T. (2004). Odorous swine wastewater treatment by purple non-sulfur bacteria, Rhodopseudomonas palustris, isolated from eutrophicated ponds. Biotechnology Letters, 26: 819-822.

Kimura M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16: 111-120.

Kumar S., Tamura K., Jakobsen I.B., Nei M. (2004). MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics, 5: 150-163.

Lee I.Y., Collins M.L. (2003). Submetido: 15/Setembro/2003, Biological Sciences, University of Wisconsin-Milwaukee, PO Box 413, Milwaukee, WI 53201, USA.

Madigan M.T., Martinko J.M., Parker J. (2006). Brock biology of microorganisms. 11ed. Prentice Hall, Inc USA. 640 p.

Madigan M.T. (2006). Microbiology, physiology, and ecology of phototrophic bacteria. In: J.M. Martinko, J. Parker (Eds.). Biology of anaerobic microorganisms. 1 ed. USA. 750 p.

Tchobanoglous G. (2016). Tratamento de efluentes e recuperaí§í£o de recursos. 5° Ed. Mcgraw-Hill. 2008 p.

Muyzer G., Waal E.C., Uitterlinden G. (1993). Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S RNAr. Applied and Environmental Microbiology, 59: 695-700.

Nagashima K.V. (1998). Primary structure of genes encoding light-harvesting and reaction. Submetido: 15/Setembro/2003. University, Faculty of Science, Department of Biology, Minamiohsawa 1-1, Hachioji, Tokyo 192-03, Japan.

Nagashima K.V., Hiraishi A., Shimada K. (1997). Horizontal transfer of genes coding for the photosynthetic reaction centers of purple bacteria. Journal of Molecular Evolution, 45: 131-136.

Ng W.J., Chin K.K., Wong K.K. (1989). Biological treatment alternatives for piggery wastes. International Biossystems, (6): 132-136.

Oz A., Sabehi G., Koblízek M. (2005). Roseobacter-like bacteria in red and mediterranean sea aerobic anoxygenic photosynthetic. Applied and Environmental Microbiology, 71: 344-353.

Ranchou-Peyruse A., Herbert R., Caumette P., Guyoneaud R. (2006). Comparison of cultivation-dependent and molecular methods for studying the diversity of anoxygenic purple phototrophs in sediments of an eutrophic brackish lagoon. Environmental Microbiology, 8: 1590-1599.

Saitou N., Nei M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4(4): 406-25.

Staley M.P., Bryant M.P., Pfennig N., Holt J.G. (1989). Bergey's Manual of Systematic Bacteriology. Williams and Wilkins, Baltimore, 3: 1635-1709.

von Sperling M. (1996). Lagoas de estabilizacao. Departamento de Engenharia Sanitaria e Ambiental. Universidade Federal de Minas Gerais. Brazil. 140 p.

Wan C.Y., De Weber H., Diels L., Thoeye C., Liang J.B., Huang L.N. (2011). Biodiversity and population dynamics of microorganisms in a full-scale membrane bioreactor for municipal wastewater treatment. Water Research, 45: 1129-1138.

Wieland A., Kuhl M., Mcgowan L., Fourí§ans A. (2003). Microbial mats on the Orkney Islands revisited: Microenvironment and microbial community composition. Microbial Ecology, 46: 371-390.

Yutin N., Suzuki M.T., BEJA O. (2000). Novel primers reveal a wider diversity among marine aerobic. Submetido: 01/Junho/2005.

Zehnder A.J.B. (1988). Biology of Anaerobic Microorganisms. 1 ed. USA. 645 p.