Effects of sublethal exposure to new pesticides lufenuron and flonicamid on common carp, Cyprinus carpio, hydromineral balance to further saltwater exposure

Melika Ghelichpour, Ali Taheri Mirghaed

Abstract

The effects of 21 days exposure to lufenuron and flonicamid were investigated on common carp responses to saltwater exposure. Fish were assigned in three groups: control (21 days in freshwater), lufenuron (exposed for 21 days) and flonicamid (exposed for 21 days). After 21 days, all fish were subjected to 15 g/l saltwater for further 4 days. Plasma glucose, cortisol, sodium, potassium, chloride, calcium and phosphorus levels were monitored 0, 24, 72 and 120 h after saltwater exposure. Type of pesticides and time of salt water exposure had interaction on plasma glucose and cortisol levels. These two stress indicators were increased significantly in all groups after saltwater exposure. The cortisol elevation continued until 72 h after salinity challenge and then decreased significantly after 120 h salinity exposure in all treatments. Plasma glucose level showed fluctuation during saltwater exposure. It was increased after 24 h saltwater exposure, then decreased after 72 h and again it was increased after 120 h salinity challenge. Increased plasma sodium showed significant elevation along with elongation of saltwater exposure. Pesticide exposure significantly affected plasma chloride levels as the flonicamid group had significantly lower chloride compared to the control and lufenuron groups. Plasma chloride showed significant elevation along with elongation of saltwater exposure. Pesticide and time of salinity challenge had interaction on plasma calcium levels as 24 h after salinity challenge calcium level of pesticide groups increased significantly. Along with elongation of saltwater exposure, calcium level of pesticides treatments decreased but it higher than the pre salinity challenge. Plasma phosphorus level increased 24 h after saltwater exposure and decreased along with elongation of saltwater exposure. In conclusion, lufenuron and flonicamid induce stress and alter gill function and blood ionic homeostasis during saltwater exposure.

Keywords

Lufenuron, Flonicamid, Adaptation, Stress, Biochemical assay.

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References

Aluru N., Vijayan M.M. (2009). Stress transcriptomics in fish: a role for genomic cortisol signaling. General and Comparative Endocrinology, 164: 142-150.

Banaee M., Haghi B.N., Ibrahim A.T.A. (2014). Sub-lethal toxicity of chlorpyrifos on Common carp, Cyprinus carpio (Linnaeus, 1758): Biochemical response. International Journal of Aquatic Biology, 1: 281-288.

Barton B.A. (2002). Stress in fishes: A diversity of responses with particular reference to changes in circulating corticosteroids. Integrative and Comparative Biology, 42: 517-525.

Bonga S.W., Lock R. (1991). Toxicants and osmoregulation in fish. Netherlands Journal of Zoology, 42: 478-493.

Bonga S.W. (1997). The stress response in fish. Physiological Reviews, 77: 591-625.

Ghelichpour M., Mirghaed A.T., Mirzargar S.S., Joshaghani H., Mousavi H.E. (2018). Modification of saltwater stress response in Cyprinus carpio (Linnaeus, 1758) pre-exposed to pesticide indoxacarb. Ecotoxicology and Environmental Safety, 147: 139-143.

Hoseini S.M., Hosseini S.A. (2010). Effect of dietary l-tryptophan on osmotic stress tolerance in common carp, Cyprinus carpio, juveniles. Fish Physiology and Biochemistry, 36: 1061-1067.

Hoseini S.M., Tarkhani R. (2013). Effect of short‐term treatment with potassium permanganate on stress markers and blood biochemistry in goldfish Carassius auratus. Aquaculture Research, 44: 869-875.

Hoseini S.M., Rajabiesterabadi H., Tarkhani R. (2015). Anaesthetic efficacy of eugenol on iridescent shark, Pangasius hypophthalmus (Sauvage, 1878) in different size classes. Aquaculture Research, 46: 405-412.

Hoseini S.M., Rajabiesterabadi H., Kordrostami S. (2016). Chronic exposure of Rutilus rutilus caspicus fingerlings to ambient copper: Effects on food intake, growth performance, biochemistry and stress resistance. Toxicology and Industrial Health, 32: 375-383.

Hoseini S.M., Tort L., Abolhasani M.H., Rajabiesterabadi H. (2018). Physiological, ionoregulatory, metabolic and immune responses of Persian sturgeon, Acipenser persicus (Borodin, 1897) to stress. Aquaculture Research, 47: 3729-3739.

Hosseini S.A., Hoseini S.M. (2012). Effect of acute crowding stress on subsequent osmotic challenge and recovery in juvenile common carp Cyprinus carpio (Linnaeus). Comparative Clinical Pathology, 21: 583-588.

Jalali M.A., Hosseini S.A., Imanpour M.R. (2010). Physiological characteristics and stress resistance of great sturgeon (Huso huso) juveniles fed with vitamins C, E, and HUFA-enriched Artemia urmiana nauplii. Fish Physiology and Biochemistry, 36: 555-564.

Kaneko T., Hirano T. (1993). Role of prolactin and somatolactin in calcium regulation in fish. The Journal of Experimental Biology, 184: 31-45.

Madsen S.S. (1990). Effect of repetitive cortisol and thyroxine injections on chloride cell number and Na+/K+-ATPase activity in gills of freshwater acclimated rainbow trout, Salmo gairdneri. Comparative biochemistry and physiology Part A: Physiology, 95: 171-175.

McCormick S.D. (1990). Cortisol directly stimulates differentiation of chloride cells in tilapia opercular membrane. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 259: R857-R863.

Saghali M., Hoseini S., Hosseini S., Baqraf R. (2014). Determination of heavy metal (Zn, Pb, Cd and Cr) concentration in benthic fauna tissues collected from the Southeast Caspian Sea, Iran. Bulletin of Environmental Contamination and Toxicology, 92: 57-60.

Shaw B.J., Handy R.D. (2011). Physiological effects of nanoparticles on fish: a comparison of nanometals versus metal ions. Environment International, 37: 1083-1097.

Simakani P., Abolhasani M.H., Hoseini S.M. (2018). Determination of mancozeb toxicity and biochemical effects in common carp (Cyprinus carpio). International Journal of Aquatic Biology, 6: 157-161.

Staetz C., Black B., Hayashi J., Kinne L., Kelly G., Treacy K., Joost H. (2006). Flonicamid: a novel mode of action for piercing sucking insects, Beltwide Cotton Conferences, pp: 1583-1590.

Taheri Mirghaed A., Ghelichpour M., Mirzargar S.S., Joshaghani H., Ebrahimzadeh Mousavi H. (2018). Toxic effects of indoxacarb on gill and kidney histopathology and biochemical indicators in common carp (Cyprinus carpio). Aquaculture Research, 49: 1616-1627.

Van der Linden A., Verhoye M., Pörtner H.-O., Bock C. (2004). The strengths of in vivo magnetic resonance imaging (MRI) to study environmental adaptational physiology in fish. Magnetic Resonance Materials in Physics, Biology and Medicine, 17: 236-248.

Wang T., Long X., Liu Z., Cheng Y., Yan S. (2015). Effect of copper nanoparticles and copper sulphate on oxidation stress, cell apoptosis and immune responses in the intestines of juvenile Epinephelus coioides. Fish and Shellfish Immunology, 44: 674-682.

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