DNA barcoding of Aphanius vladykovi from different habitats in Chaharmahal va Bakhtiari Province, Iran

Farideh Abolhasani; Iraj Hashemzadeh; Mohamad Saieed Heidarnejad; Seyedeh Narjes Tabatabaei

doi:10.22034/ijab.v8i1.612

Authors

Farideh Abolhasani Department of Biology, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran.
Iraj Hashemzadeh
[email protected]
Department of Fisheries and Environmental sciences, Faculty of Natural Resources and Earth Sciences, Shahrekord University, Shahrekord, Iran.
Mohamad Saieed Heidarnejad Department of Biology, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran.
Seyedeh Narjes Tabatabaei Department of Biodiversity and Ecosystem Management, Environmental Sciences Research Center, Shahid Beheshti University, Tehran, Iran.

Vol. 8 No. 1 (2020): February

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February 25, 2020

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This study was aimed to reveal the possible cryptic diversity of the Aphanius vladykovi populations in the Chaharmahal va Bakhtiari Province, Iran using mitochondrial cytochrome-c oxidase subunit I (COI). A total of 30 specimens from the Beheshtabad River, Choghakhor and Gandoman Wetlands, and Brovi, Shalamzar, and Balagholi springs from the Chaharmahal va Bakhtiari Province were collected. The maximum within-population genetic distance based on K2P was 0.28% and this distance was 0.22% between populations of Gandoman and Brovi with Shalmazar, whereas the least genetic distance was observed between Choghakhor and Beheshtabad (0.09%). A total of six haplotypes were observed between the studied specimens. Maximum Likelihood (ML) and Neighbor-Joining (NJ) trees reconstructed and all haplotypes from A. vladykovi specimens collected from non-type localities nested in one group with A. vladykovi from Choghakhor wetland i.e. type locality. The results of this study detected no cryptic diversity in A. vladykovi inhabiting different habitats in the studied region. Hence, it is proposed to consider all the studied populations in conservation measures related to A. vladycovi.

Abolhasani, F., Hashemzadeh, I., Heidarnejad, M. S., & Tabatabaei, S. N. (2020). DNA barcoding of Aphanius vladykovi from different habitats in Chaharmahal va Bakhtiari Province, Iran. International Journal of Aquatic Biology, 8(1), 66–72. https://doi.org/10.22034/ijab.v8i1.612

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Aljanabi S.M., Martinez I. (1997). Universal and rapid salt-extraction of high-quality genomic DNA for PCR-based techniques. Nucleic Acids Research, 25: 4692-4693.

Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25(17): 3389-3402.

Asgharian H., Hosseinzadeh Sahafi H., Ashja A., SHekarriz S.H., Elahi E. (2011). Cytochrome c oxidase subunit 1 barcode data of fish of the Nayband National Park in the Persian Gulf and analysis using meta-data flag several cryptic species. Molecular Ecology Resources, 11: 461-472.

Cavraro F., Malavasi S., Torricelli P., Gkenas C., Liousia V., Leonardos I., Kappas I., Abatzopoulos T.J., Triantafyllidis A. (2017). Genetic structure of the South European toothcarp Aphanius fasciatus (Actinopterygii: Cyprinodontidae) populations in the Mediterranean basin with a focus on the Venice Lagoon. The European Zoological Journal, 84: 153-166.

í‡ií§ek E., Fricke R., Sungur S., Eagderi S. (2018). Endemic freshwater fishes of Turkey. FishTaxa, 3(4): 1-39.

Coad W.B. (2017). Freshwater Fishes of Iran. Available at WWW.braincoad. Com/contents.htm.

Dabert M. (2006). DNA markers in the phylogenetics of the Acari. Biological Letter, 43: 97-107.

Esmaeili H.R., Asrar T., Gholamifard A. (2018). Cyprinodontid fishes of the world: an updated list of taxonomy, distribution and conservation status (Teleostei: Cyprinodontoidea). Iran Journal of Ichthyology, 5: 1-29.

Esmaeili H.R., Teimori A., Gholami Z., Reichenbacher B. (2014). Two new species of the tooth-carp Aphanius (Teleostei: Cyprinodontidae) and the evolutionary history of the Iranian inland and inland-related Aphanius species. Zootaxa, 3786: 246-268.

Hajibabaei M., Janzen D.H., Burns J.M., Hallwachs W., Hebert P.D.N. (2006). DNA barcodes tropical Lepidoptera. Proceedings of the National Academy of Sciences, 103: 968-971.

Hashemzadeh Segherloo I., Bernatchez L., Golzarianpour K., Abdoli A., Primmer C.R., Bakhtiary M. (2012b). Genetic differentiation between two sympatric morphs of the blind Iran cave barb Iranocypris typhlops. Journal of Fish Biology, 81(5): 1747-1753.

Hashemzadeh Segherloo I., Farahmand H., Abdoli A., Bernatchez L., Primmer R., Swatdipong A., Karami M., Khalili B. (2012a). Phylogenetic status of brown trout Salmo trutta populations in five rivers from the southern Caspian Sea and two inland lake basins, Iran: a morphogenetic approach. Journal of Fish Biology, 81: 1479-1500.

Hubert N., Hanner R., Holm E., Mandrak N.E., Laviolette N., Taylor E., Burridge M., Watkinson D., Curry A., Bentzen P., Zhang J., April J., Bernatchez L. (2008). Identifying Canadian freshwater fishes through DNA Barcodes. PLOS One, 3: p.e2490.

Ivanova N.V., Zemlak T.S., Hanner R.H., Hebert P.D.N. (2007). Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes, 7(4): 544-548."

Kerr K.C.R., Stoeckle M.Y., Dove C.J., Weigt L.A., Francis C.M., Hebert P.D.N. (2007). Comprehensive DNA barcode coverage of North American birds. Molecular Ecology Notes, 7: 535-716.

Kumar S., Stecher G., Tamura K. (2016). MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7): 1870-1874.

Ratnasingham S., Hebert P.D.N. (2007). BOLD: The Barcode of Life Data System. Available from: WWW. Boldsystems.org Retrieved 3/7/2019.

Rezvani Gilkolaei S. (1997). Molecular population genetic studies of sturgeon species in the south Caspian Sea. Ph.D. thesis, University of Wales, Swansea.

Rozas J., Ferrer-Mata A., Sánchez-DelBarrio J.C., Guirao-Rico S., Librado P., Ramos-Onsins S.E., Sánchez-Gracia A. (2017). DnaSP 6: DNA sequence polymorphism analysis of large data sets. Molecular Biology and Evolution, 34(12): 3299-3302.

Silvestro D., Michalak I. (2012). RaxmlGUI: a graphical front-end for RAXML. Organisms Diversity & Evolution, 12(4) :335-337.

Tala M., Jazemi Damane B., Laloie F., Soltani M., Azad M., Koochaki A. (2011). Investigation on polymorphism of mitochondrial genome in Cobia fish Rachycentron canadum in the northern waters of the Persian Gulf and Oman Sea. Pejouhandeh, 16(5): 246-251.

Teimori A., Jawad L.A.J., Al-Kharusi L.H., Al-Mamry J.M., Reichenbacher B. (2012). Late Pleistocene to Holocene diversification and historical zoogeography of the Arabian killifish (Aphanius dispar) inferred from otolith morphology. Scientia Marina, 76(4): 637-645.

Teimori A., Motamedi M., Iranmanesh A. (2018). Comparative morphology of urohyal bone in brackish water species of the genus Aphanius Nardo, 1827 in the Persian Gulf and Southeastern Mediterranean Sea basins (Teleostei: Aphaniidae). Mediterranean Marine Science, 19(2): 356-365.

Ward R.D., Zemlak T.S., Innes B.H., Last P.R., Hebert P.D.N. (2005). DNA barcoding Australia s fish species. Philosophical Transactions of the Royal Society B Philosophical Transactions of the Royal Society B, 360: 1847-1857.

Yang L., Mayden R.L., Sado T., Saitoh K., Miya M. (2010). Molecular phylogeny of the fishes traditionally referred to Cyprinini sensu stricto (Teleostei: Cypriniformes). Zoologaic Scripta, 39: 527-550.