Sub-lethal effects of potassium dichromate on hematological and histological parameters in climbing perch, Anabas testudineus (Anabantidae)

Liya Vazhamattom Benjamin, Ranjeet Kutty

Abstract

Chromium, which enters the river through anthropogenic sources, is one of the potent heavy metals. The present study is an attempt to determine the LC50 of Potassium dichromate for the climbing perch, Anabas testudineus and to study the impact of two sub-lethal concentrations (6 and 12 mg/l) of Potassium dichromate the toxic hexavalent Cr(VI) form of Chromium on this fish through investigating hematological and histopathological parameters. Experimental set up included quadruplicate treatments for each dosage, and the results were compared with control treatments. The results showed that the LC50 value at 96 hr was 59.92 mg/l. The fishes exposed to sub-lethal concentrations showed severe abnormalities such as; degeneration of hepatocytes, necrosis of hepatic tissue and extensive haemorrhage in gills and renal tissue. The present study brings out the harmful impact of Cr(VI) in the aquatic environment and necessitates regulations of its inflow to natural water bodies as a management plan to curb its contamination.

Keywords

Heavy metal, Hematology, Histopathology, Chromium.

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References

Agrawal A., Ravi S.P., Bechan S. (2010). Water pollution with special reference to pesticide contamination in India. Journal of Water Resource and Protection, 2: 432-448.

APHA. (2012) Standard Methods for the Examination of Water and Waste Water, 22nd edn. American Public Health Association, American Water Works Association, Water Environment Federation. Washington, DC.

Begum G., Rao J.V., Srikanth K. (2006). Oxidative stress and changes in locomotor behavior and gill morphology of Gambusia affinis exposed to chromium. Toxicological and Environmental Chemistry, 88: 355-365.

Bhatkar N.V. (2011). Chromium, nickel and zinc induced histopathological alterations in the liver of Indian common carp Labeo rohita (Ham.) Journal of Applied Sciences and Environmental Management, 15(2): 331-336.

Brungs W.A., McCormick J.H., Neiheisel T.W., Spehar R.L., Stephan C.E., Stokes G.N. (1977). Effects of pollution on freshwater fish. Journal of the Water Pollution Control Federation, 49: 1425-1493.

Conacher H.B., Page B.D., Ryan J.J. (1993). Industrial chemical contamination of food. Food Additives and Contaminants,10(1): 129-143.

Dacie J.V., Lewis S.M. (1982). Blood cell morphology in health and disease. Practical haematology, 6th edn. Churchill Livingstone, London, UK.

Finney D.J. (1952). Probit Analysis, 2nd edn. Cambridge University Press, New York. 318 p.

Hedayati A., Safahieh A., Savari A., Marammazi J.G. (2010). Detection of range finding test of mercury chloride in yellow sea bream (Acanthopagrus latus). Iranica Journal of Energy and Environment, 1: 228-233.

Hesser E.F. (1960). Methods for routine on fish haematology. Progressive Fish-Culturist, 22: 164-171.

Irwin R.J., Van Mouwerik M., Stevens L., Seese M.D., Basham W. (1997). Chromium VI (hexavalent chromium). Environmental contaminants encyclopedia, National Park Service, Water Resources Division, Fort Collins, Colorado.

Kerala State Pollution Control Board (2016). Water and air quality directory, Trivandrum, India.

Kurtović B., Teskeredžić E., Teskeredžić Z. (2008). Histological comparison of spleen and kidney tissue from farmed and wild European sea bass (Dicentrarchus labrax L.). Acta Adriatica, 49: 147–154.

Mallesh B., Pandey P.K., Kundan K.A., Vennila S.K. (2015). Bioconcentration of hexavalent chromium in Cirrhinus mrigala (Ham 1822) effect on haematological parameters. Journal of Earth Science, 5(1): 59-67.

Mini V.S. (2015). Haematological changes in a freshwater fish, Anabas testudineus (Bloch) on exposure to heavy metal toxicant Cadmium chloride. Asian Journal Of Science And Technologies, 6(1): 988-992.

Palmer C.D., Wittbrod P.R. (1991). Processes affecting the remediation of chromium-contaminated sites. Environmental Health Perspectives, 92: 25-40.

Ranjeet K., Das D., Shamsiya M.P., Reshmi K. (2013). Effect of ekalux toxicity on selected physiological parameters in Anabas testudineus. Applied Ecology and Environmental Research, 11(4): 569-580.

Risikesh T., Sharma G.D., Dwivedi B.S., Khatik S.K. (2007). Chromium as a pollutant. Industrial Pollution Control, 23(2): 209-215.

Sharma J., Langer S. (2014). Effect of Manganese on haematological parameters of fish, Garra gotyla gotyla. Journal of Entomology and Zoology Studies, 2(3): 77-81.

Smith C. (1968). Hematological changes in coho salmon fed on folic acid deficient dietJournal of the Fisheries Research Board of Canada, 25(1): 151-156.

Tchounwou P.B., Yedjou C.G., Patlolla A.K., Sutton D.J. (2012). Heavy metal toxicity and the environment. In: Molecular, clinical and environmental toxicology. Springer, Berlin.

Velez D., Montoro R. (1998). Arsenic speciation in manufactured seafood product: A review. Journal of Food Protection, 61(9): 1240-1245.

Velma V., Vutukuru S.S., Tchounwou P.B. (2009). Ecotoxicology of hexavalent Chromium in freshwater fish: A critical review. Reviews on Environmental Health, 24(2): 129–145.

World Water Assessment Programme (WWAP). (2018). Nature-based solutions for water. United Nations Educational, Scientific and Cultural Organization, Paris.

Zayed A.M., Terry N. (2003). Chromium in the environment: factors affecting biological remediation. Plant and Soil, 249: 139–156.

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