Study on probiotic potential of Bacillus species isolated from the intestine of farmed rainbow trout, Oncorhynchus mykiss

Mina Seifzadeh; Mohammad Rabbani Khorasgani; Rasoul Shafiei

doi:10.22034/ijab.v7i3.584

Authors

Mina Seifzadeh
[email protected]
Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
Mohammad Rabbani Khorasgani Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran
Rasoul Shafiei Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran

Vol. 7 No. 3 (2019): June

Articles

June 25, 2019

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The gastrointestinal tract of fishes is a complex ecosystem occupied by a large number of microorganisms, some of them could have potentially-valuable features. This research was conducted to study Bacillus species in the intestine of farmed rainbow trout to examine their probiotic properties, and to provide a new source of probiotics. A total of 23 farmed rainbow trout were sampled and their intestine samples were cultured. Following the morphological assay and biochemical analysis, isolated Bacilli were amplified by polymerase chain reaction and universal primers 27f and 1492r. Bacillus subtilis and B. amyloliquefaciens were isolated from 5 and 3 samples, respectively. Bacillus tequilensis, B. cereus and B. licheniformis were isolated from 1 sample. Probiotic properties of B. subtilis strain MSM 24, B. amyloliquefaciens strain TMM 25 and B. licheniformis strain MR 78 were confirmed. Since probiotic bacteria cause no foodborne diseases, their existence in farmed trout intestines, and their penetration into the fish tissues do not pose any risk to consumers' health.

Seifzadeh, M., Rabbani Khorasgani, M., & Shafiei, R. (2019). Study on probiotic potential of Bacillus species isolated from the intestine of farmed rainbow trout, Oncorhynchus mykiss. International Journal of Aquatic Biology, 7(3), 166–174. https://doi.org/10.22034/ijab.v7i3.584

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Cao H., He S., Wei R., Diong M., Lu L. (2011). Bacillus amyloliquefaciens G1: a potential antagonistic bacterium against Eel-pathogenic Aeromonas hydrophila. Evidence-Based Complementary and Alternative Medicine, 1-7.

Chandrasekara Bhagya C.H.W.M.R., Wijesundera Sulochana W.S., Perera Hemamal N. (2013). Polymerase chain reaction optimization for amplification of Guanine-Cytosine rich templates using buccal cell DNA. Indian Journal Human Genetics, 19: 78-83.

Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I. (2007). Comparative analysis of the complete genome sequence of the plant growth promoting bacterium Bacillus amyloliquefaciens FZB42. Natural Biotechnology, 25: 1007-1014.

Chen Y., Li J., Xiao P., Zhu W., Mo Z. (2016). The ability of marine Bacillus spp. isolated from fish gastrointestinal tract and culture pond sediment to inhibit growth of aquatic pathogenic bacteria. Iranian Journal Fisheries Science, 15: 701-714.

Cohen P.S., Laux D.C. (1995). Bacterial adhesion to and penetration of intestinal mucus in vitro. Methods in Enzymology, 253: 309-314.

Dashti A.A., Jadaon M.M., Abdulsamad A.M., Dashti H.M. (2009). Heat treatment of bacteria: a simple method of DNA extraction for molecular techniques. Kuwait Medical Journal, 41: 117-122.

Earl A.M., Losick R., Kolter R. (2008). Ecology and genomics of Bacillus subtilis. Trends in Microbiology, 16: 269-280.

Efendi Y., Usra Y. (2014). Bacillus subtilis strain VITNJ1 potential probiotic bacteria in the gut of Tilapia (Oreochromis niloticus) are cultured in floating net, Maninjau Lake, West Sumatra. Pakistan Journal Nutrition, 13: 710-715.

Elshaghabee F.M.F., Rokana N., Gulhane R.D., Sharma C., Panwar H. (2017). Bacillus as potential probiotics: status, concerns, and future perspectives. Frontiers Microbiology, 8: 1490-1505.

Ganguly S., Prasad A. (2011). Microflora in fish digestive tract plays significant role in digestion and metabolism. Reviews in Fish Biology and Fisheries, 22: 11-16.

Ghorbanzadeh R.A., Nazari S. (2015). Statistical yearbook of Iranian fisheries during 2013-2014. Iranian Fisheries Organization, Tehran. 72 p.

Holt J.G., Krieg N.R. (1994). Bergey's manual of determinative bacteriology. Williams- Wilkins, Com., Baltimore, M. 950 p.

Hovda M.B., Lunestad B.T., Fontanillas R., Rosnes J.T. (2007). Molecular characterization of the intestinal microbiota of farmed Atlantic salmon (Salmo salar L.). Aquaculture, 272: 581-588.

Huber I., Spanggaard B., Appel K.F., Rossen L., Nielsen T., Gram L. (2004). Phylogenetic analysis and in situ identification of the intestinal microbial community of rainbow trout (Oncorhynchus mykiss, Walbaum). Journal Applied Microbiology, 96: 117-132.

Iranian National Standard No. 19459. (2014). Probiotic microorganisms specifications and test methods. Iranian Standards and Industrial Research Institute, Tehran. 10 p.

Kavitha M., Raja M., Perumal P. (2018). Evaluation of probiotic potential of Bacillus spp. isolated from the digestive tract of freshwater fish Labeo calbasu (Hamilton, 1822). Aquaculture Report, 11: 59 -69.

Krishnan R. (2014). Probiotic potential of Bacillus species isolated from freshwater fish Anabas testudineus in Labeo rohita. International Journal Research Development, 1: 46-50.

Lalloo L., Ramchuran S., Ramduth D., Gorgens J., Gardiner N. (2007). Isolation and selection of Bacilluss pp. as potential biological agents for enhancement of water quality in culture of ornamental fish. Journal Applied Microbiology, 103: 1471-1479.

Lane D.J., Pace B., Olsen G.J., Stahlt D.A., Sogint M., Pace N.R. (1985). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proceedings of the National Academy of Sciences, 82: 6955-6959.

Mohkam M., Amini S.A., Shokri D., Berenjian A., Rahimi F., Sadraeiian M., Khalvati B., Gholami A., Ghasemi,Y. (2016). Characterization and in vitro probiotic assessment of potential indigenous Bacillus strains isolated from soil rhizosphere. Minerva Biotecnologica, 28: 19-28.

Mongkolthanaruk W. (2012). Classification of Bacillus beneficial substances related to plants, humans and animals. Journal Microbiology Biotechnology, 22: 1597-1604.

Newaj-Fyzul A., Adesiyun A.A., Mutani A., Ramsubha A., Brunt J., Austin B. (2007). Bacillus subtilis AB1 controls Aeromonas infection in rainbow trout (Oncorhynchus mykiss, Walbaum). Journal Applied Microbiology, 103: 1699-706.

Nithya V., Halami P.M. (2013). Evaluation of the probiotic characteristics of Bacillus species isolated from different food sources. Annual Microbiology, 63: 129-137.

Pasmik D.J., Evans J.J., Klesius P.H. (2008). Bacillus licheniformis isolated during a fish kill is non"pathogenic. Fisheries Science, 74: 1351-1353.

Ran C., Carrias A., Williams M.A., Capps N., Dan B.C.T., Newton J.C. (2012). Identification of Bacillus strains for biological control of Catfish pathogens. PLoS One, 7: e45793.

Rasool U., Ahmad A., Badroo G.A., Mudasir M., Fayaz S., Mustafa R. (2017). Isolation and identification of Bacillus cereus from fish and their handlers from Jammu, India. International Journal Current Microbiology Applied Science, 6: 441-447.

Reva O.N., Dixelius C., Meijer J., Priest F.G. (2004). Taxonomic characterization and plant colonizing abilities of some bacteria related to Bacillus amyloliquefaciens and Bacillus subtilis. FEMS. Microbiology Ecology, 48: 249-259.

Rowan N.J., Deans K., Anderson J.G., Gemmell C.G., Hunter I.S., Chai-thong T. (2001). Putative virulence factor expression by clinical and food isolates of Bacillus spp. after growth in reconstituted infant milk for¬mulae. Applied Environmental Microbiology, 67: 3873-81.

Silva M.A.C., Cavalett A., Spinner A., Rosa D.C., Jasper R.B., Quecine M.C. (2013). Phylogenetic identification of marine bacteria isolated from deep-sea sediments of the eastern South Atlantic Ocean. Springer Plus, 2: 127-137.

Sugita H., Hirose Y., Matsuo N., Deguchi Y. (1998). Production of the antibacterial substance by Bacillus sp. Strain NM 12, an intestinal bacterium of Japanese coastal fish. Aquaculture, 165: 269-280.

Teng L.J., Hsueh P.R., Huang Y., Tsai J.C. (2004). Identification of bactericides the taiotaomicron on the basis of an unexpected specific amplicon of universal 16S ribosomal DNA PCR. Journal Clinical Microbiology, 42: 1727-1730.

Turner S., Pryer K.M., Miao V.P.W., Palmer J.D. (1999). Investigating deep phylogenetic relationships among cyanobacteria and plastids by small subunit rRNA sequence analysis. Journal Eukaryotic Microbiology, 46: 327-338.