Hemato-immunological, serum metabolite and enzymatic stress response alterations in exposed rainbow trout (Oncorhynchus mykiss) to nanosilver

Nanosilver Hematology Serum metabolites Cortisol Oxidative stress.

Authors

  • Ali Taheri Mirghaed Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Peyman Yarahmadi Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Mitra Shabrang harehdasht Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
  • Paul M. Craig Department of Biology, Faculty of Science, University of Waterloo, Waterloo, Ontario, Canada.
  • Hamed Ghafari Farsani Young Reasearchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
  • Nahid Ghysvandi Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
  • Soheil Eagderi Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
August 25, 2018

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The aim of present study was to investigate the effects of sub-lethal concentrations of silver nanoparticles (AgNP) on hematological parameters, differential tests of white blood cells, serum metabolite parameters, serum enzymes activity and serum ions in rainbow trout, Oncorhynchus mykiss. Healthy rainbow trout, were exposed to sub-lethal concentrations (0, 1.5 and 2.5 ppm) of nanosilver for 14 days. RBC, WBC and Hct levels were significantly (P<0.05) increased in exposed groups. Within the white blood cells, only neutrophils showed a significant increase at 7 and 14 days post exposure (P<0.05). Serum triglyceride, total serum protein, albumin and globulin levels were decreased (P<0.05) in exposed fish, however, cholesterol levels increased in the 2.5 ppm group at 7 days after exposure (P<0.05). Cortisol and glucose increased significantly at 7 and 14 days of exposure in both concentrations of AgNPs (P<0.05). Decreases in serum ions level were observed, although reduction in chloride ions occurred earlier and more severe than other measured parameters (P<0.05). Elevation in serum ALP, LDH, ALT and AST enzymes were observed during the experiment (P<0.05), although SOD and CAT activity were significantly decreased in exposed groups (P<0.05). The results revealed that AgNP can affect the hematological, serum metabolite and enzymatic parameters of O. mykiss, as well as AgNP exposure induce a general oxidative stress response in O. mykiss.