Effect of probiotic Pediococcus acidilactici on growth, reproductive and bacterial count of marine rotifer Brachionus plicatilis

Yosra Abbasi; Nasrollah Ahmadifard; Amir Tukmechi

doi:10.22034/ijab.v7i1.489

Authors

Yosra Abbasi Department of Fisheries, Faculty of Natural Resources, Urmia University, Urmia, Iran.
Nasrollah Ahmadifard
[email protected]
Department of Fisheries, Faculty of Natural Resources, Urmia University, Urmia, Iran.
Amir Tukmechi Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

Vol. 7 No. 1 (2019): February

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February 26, 2019

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This study investigated the effects of different concentrations of probiotic Pediococcus acidilactici on the total number, specific growth rate, attached egg number and microbial count (lactic acid and total aerobic bacteria) in culture water and body of marine rotifer Brachionus plicatilis. The experiment was conducted as a completely randomized design in four groups: first group fed probiotic at 0.5í—10⁶ CFU ml^-1(P1), the second group fed probiotic at 1í—10⁶ CFU ml^-1 (P2), the third group fed probiotic at 1.5í—10⁶ CFU ml^-1 (P3), and the last group fed without probiotic (NP, control). Rotifers were cultured in standard conditions at an initial density of 30 ind.ml^-1 using a 2.5í—10⁶ cell mL^-1of Nanochloropsis oculta. Based on the results, the maximum number (215±4.91 ind.ml^-1) of rotifers were significantly obtained after ten days in the third group in comparison to other treatments (P<0.05). In addition, the highest growth rate (0.46±0.023 ind.ml^-1) and shortest doubling time (1.5±0.14 days) were obtained in the treatment P3 but no significant difference was found between NP and other groups (P>0.05). Moreover, it was revealed that the total aerobic bacteria was significantly related to the 3rd group that found be 1.80í—10³CFU rotifer^-1 and 34.0í—10⁴CFU ml^-1 in rotifer body and culture water,respectively (P<0.05). Nonetheless, lactic acid bacteria of rotifer body and culture water was not concentration-dependent and the highest number of lactic acid bacteria in rotifer body (50.58±6.08 CFU rotifer^-1) and rotifer culture water (4.43±3.28í—10³CFU ml^-1) was obtained in the treatments of P1 and P2, respectively. In conclusion, the present study showed that the bacteria P. acidilactici with N. oculta algae provide a higher growth rate and total aerobic bacteria in rotifer B. plicatilis.

Abbasi, Y., Ahmadifard, N., & Tukmechi, A. (2019). Effect of probiotic Pediococcus acidilactici on growth, reproductive and bacterial count of marine rotifer Brachionus plicatilis. International Journal of Aquatic Biology, 7(1), 27–34. https://doi.org/10.22034/ijab.v7i1.489

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