Effects of Pirimicarb carbamate insecticide alone and in combination with lead (Pb) on biochemical parameters of soft tissues in freshwater snail, Galba truncatula

Marziyeh Raisi; Hamid Reza Pourkhabbaz; Mahdi Banaee; Alireza Pourkhabbaz; Saeideh Javanmardi

doi:10.22034/ijab.v6i3.459

Authors

Marziyeh Raisi Department of Environment, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.
Hamid Reza Pourkhabbaz
[email protected]
Department of Environment, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.
Mahdi Banaee Department of Aquaculture, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.
Alireza Pourkhabbaz Department of Environment, University of Birjand, Birjand, Iran.
Saeideh Javanmardi Department of Environment, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.

Vol. 6 No. 3 (2018): June

Articles

November 5, 2018

Downloads

PDF

Abstract
How to Cite
Metrics
References

In this study, potential effects of Pirimicarb and lead (Pb) were investigated on biochemical parameters in tissues of freshwater snails, Galba truncatula. During an 8-day experiment, snails were exposed to sub-lethal concentrations of Pirimicarb (0.5 and 1 mg/L) and/or lead acetate (0.1 and 0.2 mg/L). Biochemical analyses of tissues to Photometric method in snails indicate that snails treated with Pirimicarb, Pb, or both Pirimicarb and Pb increased malondialdehyde (MDA) and catalase (CAT) and decreased gamma-glutamyl transferase (GGT) levels, compared to the control group. Alanine transferase (ALT), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) activity were increased in combined treatments of Pirimicarb and Pb. Total antioxidant (TAO) level increased in snails exposed to both Pirimicarb and Pb, while it decreased in snails treated with either Pb or Pirimicarb. Cholesterol level increased in most experimental groups. Aspartate aminotransferase (AST) was showed no significant changes in groups treated with 0.1 and 0.2 mg/L of Pb compared to the control; however, AST enhanced in other treatments. In groups exposed to 0.5 and 1 mg/L of Pirimicarb, the inhibition of acetylcholinesterase (AChE) was not significant, although a significant reduction was found in AChE level in other treatments. The results indicated that cytotoxicity of Pirimicarb alone and in combination with Pb depended on their concentrations. Higher concentrations of Pb induced significant changes in some biochemical parameters. Moreover, increased Pb level in water intensifies toxic effects of Pirimicarb in snails. Pirimicarb or/and Pb, in sub-lethal concentrations, induced oxidative damages in soft tissue of snails. Finally, these data support the hypothesis that changes in biochemical parameters were induced by exposure to Pirimicarb or/and Pb.

Raisi, M., Pourkhabbaz, H. R., Banaee, M., Pourkhabbaz, A., & Javanmardi, S. (2018). Effects of Pirimicarb carbamate insecticide alone and in combination with lead (Pb) on biochemical parameters of soft tissues in freshwater snail, Galba truncatula. International Journal of Aquatic Biology, 6(3), 126–137. https://doi.org/10.22034/ijab.v6i3.459

Download Citation

Abbas Zadeh K. (1995). Investigation of Crabs tidal areas of Bushehr coast. Iran Fisheries Information Center. (In Persian).

Abdel-Halim K.Y., Abo El-Saad A.M., Talha M.M., Hussein A.A., Bakry N.M. (2013). Oxidative stress on land snail Helix aspersa as a sentinel organism for ecotoxicological effects of urban pollution with heavy metals. Chemosphere, 93(6): 1131-1138.

Adedeji O.B., Okocha R.C. (2011). Bioconcentration of heavy metals in prawns and water from Epe lagoon and Asejire River in southwest Nigeria. Department of Veterinary Public Health and Preventive Medicine. University of Ibadan. Nigeria Journal of Applied Sciences and Environmental Sanitation, 6(3): 377-384.

Ali D., Alarifi S., Kumar S., Ahamed M., Siddiqui M.A. (2012). Oxidative stress and genotoxic effect of zinc oxide nanoparticles in freshwater snail Lymnaea luteola L. Aquatic Toxicology, 125: 83-90.

Atailia A., Berrebbah H., Boucenna M., Alayat A., Amamra R., Grara N., Djeba M. (2016). Biomarkers responses of Land Snails Helix aspersa exposed to chronic metal pollution under field and laboratory conditions. Nature Environment and Pollution Technology, 1209-1215.

Banaee M., Beitsayah A., Jorabdoz I. (2015 a). Assessment of mercury bioaccumulation in zebra cichlid (Cichlasoma Nigrofasciatum) exposed to sublethal concentrations of permethrin. Iranian Journal of Toxicology, 8(27): 1168-1173.

Banaee M., Mohammadipour S., Madhani S. (2015b). Effects of sublethal concentrations of permethrin on bioaccumulation of cadmium in zebra cichlid (Cichlasoma nigrofasciatum). Toxicological and Environmental Chemistry, 97(2): 200-207.

Banaee M., Nemadoost Haghi B., Tahery S., Shahafve S.H., Vaziriyan M. (2016). Effects of sub-lethal toxicity of paraquat on blood biochemical parameters of common carp, Cyprinus carpio (Linnaeus, 1758). Iranian Journal of Toxicology 10(1): 31-40.

Barky F.A., Abdelsalam H.A., Mahmoud M.B., Hamdi, S.A. (2012). Influence of atrazine and roundup pesticides on biochemical and molecular aspects of Biomphalaria alexandrina snails. Pesticide Biochemistry and Physiology, 104(1): 9-18.

Basopo N., Ngabaza T. (2015). Toxicological effects of chlorpyrifos and lead on the aquatic snail Helisoma duryi. Journal Advances in Biological Chemistry, 5: 225-233.

Benzie I., Strain J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of "Antioxidant power: the FRAP assay". Analytical Biochemistry, 239: 70-76.

Bhattacharya P., Swarnakar S., Mukhopadhyay A., Ghosh S. (2016). Exposure of composite tannery effluent on snail, Pila globosa: A comparative assessment of toxic impacts of the untreated and membrane treated effluents. Ecotoxicology and Environmental Safety, 126: 45-55.

Bravo J.A., Forsythe P, Chew M.V., Escaravage E., Savignac H.M., Dinan T.G. (2011). Ingestion of Lactobacillus strain regulates emotional behavior and central GABA receptor expression in a mouse via the vagus nerve. Proc Natl Acad Sci USA, 108:16050-16055.

Burtis C.A. Ashwood E.R. (1999). Clinical chemistry. In: W.B London (Ed.). Saunders Publishing. pp: 1044-1045.

Cao G., Sofic E., Prior R.L. (1996). Antioxidant capacity of tea and common vegetables. Journal Agricultural and Food Chemistry, 44: 3426-3431.

CASAFE. (2009). La Argentina 2050. La revolucio´n tecnolo´gica del agro. Hacia el desarrollo integral de nuestra sociedad. Argentina: Ca´mara de Sanidad Agropecuaria y Fertilizantes. 744 p.

Coeurdassier M., Saint-Denis M., Gomot-de Vaufleury A., Ribera D., Badot P. M. (2001). The garden snail (Helix aspersa) as a bioindicator of organophosphorus exposure: effects of dimethoate on survival, growth, and acetylcholinesterase activity. Environmental Toxicology and Chemistry, 20(9): 1951-1957.

El-Shenawy N.S., Mohammadden A., Al-Fahmie, Z.H. (2012). Using the enzymatic and non-enzymatic antioxidant defense system of the land snail Eobania vermiculata as biomarkers of terrestrial heavy metal pollution. Ecotoxicology and Environmental Safety 84: 347-354.

Goth L. (1991). A simple method for determination of serum catalase activity and revision of reference range. Clinica Chimica Acta, 196: 143-151.

Grosell M., Gerdes R.M., Brix K.V. (2006). Chronic toxicity of lead to three freshwater invertebrates -Brachionus calyciflorus, Chironomus tentans, and Lymnaea stagnalis. Environmental Toxicology and Chemistry, 25(1): 97-104.

Gul Y., Gao Z.X., Qian X.Q., Wang W.M. (2011). Hematological and serum biochemical characterization and comparison of wild and cultured northern snakehead (Channa argus). Journal of Applied Ichthyology, 27: 122-128.

Hamidipoor F., Banaee M., Pourkhabbaz H., Javanmardi S. (2015). Synergistic effects of sub-lethal consentrations of deltamethrin on lead acetate toxicity in Japanese Quail (Coturnix japonica). Journal of Chemical Health Risks, 6(1): 9-22.

Hamidipoor F., Pourkhabbaz H.R., Banaee M., Javanmardi S. (2015). Sub-lethal toxic effects of deltamethrin on blood biochemical parameters of Japanese quail, Coturnix japonica. Toxicological and Environmental Chemistry, 97(9): 1217-1225.

Hoseini S.M., Ghelichpour M. (2011). Efficacy of clove solution on blood sampling and hematological study in Beluga, Huso huso. Fish Physiology and Biochemistry, 38: 493-8.

Ibrahim A.M., Ahmed A.K., Bakry F.A., Abdel-Ghaffar F. (2018). Hematological, physiological and genotoxicological effects of Match 5% EC insecticide on Biomphalaria alexandrina snails. Ecotoxicology and Environmental Safety, 147: 1017-1022.

Järup L. (2003). Hazards of heavy metal contamination. British Medical Bulleti,. 68:167-82.

Kamel N., Jebali J., Banni M., Ben Khedher S., Chouba L., Boussetta H. (2012). Biochemical responses and metals levels in Ruditapes decussatus after exposure to treated municipal effluents. Ecotoxicology and Environmental Safety, 82: 40-46.

Khalil A.M. (2015). Toxicological effects and oxidative stress responses in freshwater snail, Lanistes carinatus, following exposure to chlorpyrifos. Ecotoxicology and Environmental Safety, 116: 137-142.

Kletzien R.F., Harris P.K.W., Foellmi LA. (1994). Glucose-6- phosphate dehydrogenase: a "housekeeping" enzyme subject to tissue-specific regulation by hormones, nutrients, and oxidant stress. FASEB J, 8: 174-181.

Knedel M., Boetteger R. (1967). Kinetic method for determination of pseudocholinesterase (acylcholine acylhydrolase) activity. Wiener Klinische Wochenschrift, 45: 325-327.

Laznik Z., Mihicinac M., Rupnik J., Vidrih M., Prsa I., Trdan S. (2010). Testing the efficacy of different substances against Arion slugs (Arionidae) under laboratory conditions. Acta agriculturae Slovenica, 95(2). 129-140.

Leomanni A., Schettino T., Calisi A., Gorbi S., Mezzelani M., Regoli F., Lionetto M.G. (2015). Antioxidant and oxidative stress related responses in the Mediterranean land snail Cantareus apertus exposed to the carbamate pesticide Carbaryl. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 168: 20-7.

Leopold J.A., Loscalzo J. (2000). Cyclic strain modulates resistance to oxidant stress by increasing G6PDH expression in smooth muscle cells. American Journal of Physiology, 279: H2477–H2485.

Lin Y., Lin S., Guo H., Zhang Z., Chen X. (2013). Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants. Biotechnology Letters, 35: 1509-1518.

Khalil M.A. (2015). Xicological effects and oxidative stresses ponses in freshwater snail, Lanistes carinatus, following exposure to chlorpyrifos. Ecotoxicology and Environmental Safety, 116: 137-142.

Macías-Mayorga D., Laiz I., Moreno-Garrido I., Blasco J. (2015). Is oxidative stress related to cadmium accumulation in the Mollusc Crassostrea angulate? Aquatic Toxicology, 161: 231-241.

Mehrpak M., Banaee M., Nematdoost Haghi B., Noori A. (2016). Protective effects of vitamin C and chitosan against cadmium- induced oxidative stress in the liver of common carp (Cyprinus carpio). Iranian Journal of Toxicology, 9(30): 1360-7.

Mleiki A., Marigo´mez I., Trigui El Menif N. (2015). Effects of dietary Pb and Cd and their combination on Glutathion-S-Transferase and Catalase enzyme activities in digestive gland and foot of the green garden snail, Cantareus apertus (Born, 1778). Bulletin of Environmental Contamination and Toxicology, 94: 738-743.

Moss D.W., Henderson A.R. (1999). Clinical enzymology. In: C.A Burtis, E.R Ashwood (Eds.). Tietz's Textbook of Clinical Chemistry, 3rd edition. WB Saunders, Philadelphia. pp. 617-721.

Nematdoost Haghi B., Banaee M. (2017). Effects of micro-plastic particles on paraquat toxicity to common carp (Cyprinus carpio): biochemical changes. International Journal of Environmental Science and Technology, 14(3): 521-530.

Placer Z.A., Cushman L.L., Johnson B.C. (1966). Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Analytical Biochemistry, 16: 359-364.

Radwan M.A., El-Gendy K.S., Gad A.F. (2010). Biomarkers of oxidative stress in the land snail, Theba pisana for assessing ecotoxicological effects of urban metal pollution. Chemosphere, 79(1): 40-46.

Rao J.V. (2006). Biochemical alteration in euryhaline fish, Oreochromis mossambicus exposed to sub-lethal concentrations of an organophosphorus insecticide, monocrotophos. Chemosphere, 65: 1814-1820.

Rifai N., Bachoric R.S., Alperrs J.J. (1999). Lipid, Lipoproteins and apolipoprotein. In: C.A Burtis, E.R Ashwood (Eds.). Teitz textbook of clinical chemistry 3rd ed. Philadelphia: W.B Saunders Company. pp: 809-861.

Ruelas-Insunza J.R., Pa´ez-Osuna F. (2000). Comparative bioavailability of trace metals using three filter-feeder organisms in a subtropical coastal environment (Southeast Gulf of California). Environmental Pollution, 107: 437-444.

Seyit A., Nejdet G., Harun Y. (2000). Natural and experimental infections of Campylobacter cryaerophila in rainbow trout: gross pathology, bacteriology, clinical pathology and chemotherapy. Fish Pathology, 35: 117-123.

Silva C.O., Simíµes T., Novais S.C., Pimparel I., Granada L., Soares A.M., Lemos M.F. (2017). Fatty acid profile of the sea snail Gibbula umbilicalis as a biomarker for coastal metal pollution. Science of the Total Environment, 586: 542-550.

Vera-Candioti J., Natale G., Soloneski S., Ronco A.E., Larramendy M.L. (2010). Sublethal and lethal effects on Rhinella arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida. Chemosphere, 78: 249-255.

Walker S.F., Baldi Salas M., Jenkins D., Garner T.W and others. (2007). Environmental detection of Batrachochytrium dendrobatidis in a temperate climate. Diseases of Aquatic Organisms, 77: 105-112.

Wan R., Meng F., Fu W., Wang Q., Su E. (2015). Biochemical responses in the gills of Meretrix meretrix after exposure to treated municipal effluent. Ecotoxicology and Environmental Safety, 111: 78-85.

Wright David A., Welbourn P. (2002). Environmental toxicology. Cambridge university press, Cambridge.U.K. http://go.mining.com/apr08-a3.

Zheng S., Zhou Q., Gao J., Xiong H., Chen C. (2012). Behavioral alteration and DNA damage of freshwater snail Bellamya aeruginosa stressed by ethylbenzene and its tissue residue. Ecotoxicology and Environmental Safety, 81. 43-8.

Zhou X., Li M., Abbas K.h., Wang W. (2009). Comparison of hematology and serum biochemistry of cultured and wild Dojo loach Misgurnus anguillicaudatus. Fish Physiology and Biochemistry, 35(3): 435-41.