Cholinesterase activity and histopatological changes in the Mediterranean crab, Carcinus maenas, exposed to environmental contaminants

Sana Ben Khedher; Zohra Haouas; Hamadi Boussetta

doi:10.22034/ijab.v5i5.379

Authors

Sana Ben Khedher
[email protected]
Laboratory of Biochemical and Environmental Toxicology, Higher Institute of Agriculture, Chott-Mariem, 4042-Sousse, Tunisia.
Zohra Haouas Laboratory of Histology Cytology and Genetics, Faculty of Medicine, Monastir 5019, Tunisia.
Hamadi Boussetta Laboratory of Biochemical and Environmental Toxicology, Higher Institute of Agriculture, Chott-Mariem, 4042-Sousse, Tunisia.

Vol. 5 No. 5 (2017): October

Articles

December 6, 2017

Downloads

PDF

Abstract
How to Cite
Metrics
References

Marine environments are continuously being threatened by a large number of pollutants including heavy metals and organophosphorous pesticides from anthropogenic sources. These compounds can cause a serious environmental problem. The present study aimed: (1) to measure sensitivity of acetylcholinesterase (AChE) activity to in vivo exposure to the organophosphorous chlorpyriphos-ethyl (CPF) and to the heavy metals cadmium (Cd) and copper (Cu) and (2) to use the histopathological lesions as tissue biomarkers for biomonitoring of different contaminations. The results clearly showed that the AChE activity in different tissues (digestive gland, muscle and eyes) of Carcinus maenas was relatively sensitive to the concentrations of CPF and tended to have different patterns in response to Cd, Cu and Cu+Cd mixture exposure. The transfer of treated crabs to the clean sea water allowed to recover totally or partially the lost activity depending on selected tissues and contaminant exposure (metals or organophosphorous compounds). Histopathological biomarkers in C. maenas exposed to different contaminants showed the presence of different lesions which altered the digestive gland after 7 days of contamination.

Ben Khedher, S., Haouas, Z., & Boussetta, H. (2017). Cholinesterase activity and histopatological changes in the Mediterranean crab, Carcinus maenas, exposed to environmental contaminants. International Journal of Aquatic Biology, 5(5), 348–359. https://doi.org/10.22034/ijab.v5i5.379

Download Citation

Abdullah A., Kumar A., Chapman J. (1994). Inhibition of acetylcholinesterase in the Australian freshwater shrimp (Paratya australiensis) by profenofos. Jornal of Environmental Toxicology Chemistry, 13: 1861-1866.

Ali M., Sreekrishnan T.R. (2001). Aquatic toxicity from pulp and paper mill effluents: a review. Journal of Advances Environmental Research, 2(5): 175-96.

Alquezar R., Markich S.J., Twining J.R. (2008). Comparative accumulation of 109Cd and 75Se from water and food by an estuarine fish (Tetractenos glaber). Journal of Environmental Radioactivity, 99: 167-180.

Banni M., Bouraoui Z., Clerandeau C., Narbonne J.F., Boussetta H. (2009). Mixture toxicity assessment of cadmium and benzo[a]pyrene in the sea worm Hediste diversicolor. Journal of Chemosphere, 77: 902-906.

Baticados M.C.L., Tendencia E.A. (1991). Effect of gusathion A on the survival and shell quality of juvenile Penaeus monodon. Journal of Aquaculture, 93: 9-19.

Bautista M.N., Lavilla-Pitogo C.R., Subosa P.F. (1994). Aflatoxin B1 contamination of shrimp feeds and its effect on growth and hepatopancreas of pre-adult Penaeus monodon. Journal of Science of Food and Agriculture, 65: 5-11.

Ben Khedher S., Jebali J., Houas Z., Nawéli H., Jrad A., Banni M., Boussetta H. (2014). Metals bioaccumulation and histopathological biomarkers in Carcinus maenas crab from Bizerta lagoon, Tunisia. Journal of Environmental Science Pollution Research, 21: 4343-4357

Bhavan P.S., Geraldine P. (2000). Histopathology of the hepatopancreas and gills of the prawn Macrobrachium malcolmsonii exposed to endosulfan. Journal of Aquatic Toxicology, 50: 331-339.

Blasco J., Puppo J. (1999). Effect of heavy metals (Cu, Cd and Pb) on aspartate and alanine aminotransferase in Ruditapes philippinarum (Mollusca: Bivalvia). Journal of Comparative Biochemistry and Physiology Part C, 122: 253-263.

Bonacci S., Corsi I., Focardi S. (2008). Cholinesterase activities in the scallop Pecten jacobaeus: Characterization and effects of exposure to aquatic contaminants. Journal of Science Total Environmental, 392: 99-109.

Botté E.S., Jerry D.R., Codi-King S., Smith-Keune C., Negri A.P. (2012). Effects of chlorpyrifos on cholinesterase activity and stress markers in the tropical reef fish Acanthochromis polyacanthus. Journal of Marine Pollution Bulletin, 65: 384-393.

Bradford M.M. (1976). A rapid and sensitive method for the quantification of microgram of protein utilizing the principal of protein-dye binding. Journal of Analytical Biochemistry, 72: 248-254.

Chouksey M.K., Kadam A.N., Zingde M.D. (2004). Petroleum hydrocarbon residues in the marine environment of Bassein-Mumbai. Journal of Marine Pollution Bulletin, 7-8(49), 637-47.

Cunha I., Mangas-Ramirez E., Guilhermino L. (2007). Effects of copper and cadmium on cholinesterase and glutathione S-transferase activities of two marine gastropods (Monodonta lineata and Nucella lapillus). Journal of Comparative Biochemistry and Physiology Part C, 145: 648-657.

De La Torre F.R., Salibian A., Ferrari L. (2000). Biomarkers assessment in juvenile Cyprinus carpio exposed to waterborne cadmium. Journal of Environmental Pollution, 109: 277-282.

Ellman G.L., Courtney K.O., Andrres V., Featherstone R.M. (1961). A new rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7: 88-95.

Elumalai M., Antunes C., Guilhermino L. (2007). Enzymatic biomarkers in the crab Carcinus maenas from the Minho River estuary (NW Portugal) exposed to zinc and mercury. Journal of Chemosphere, 66: 1249-1255.

Frasco M.F., Fournier D., Carvalho F., Guilhermino L. (2008). Does mercury interact with the inhibitory effect of dichlorvos on Palaemon serratus (Crustacea: Decapoda) cholinesterase. Journal of Science Total Environmental, 404: 88-93.

Fulton M.H., Key P.B. (2001). Acetylcholinesterase inhibition in estuarine fish and invertebrates as an indicator of organophosphorus insecticide exposure and effects. Journal of Environmental Toxicology Chemistry, 20: 37-45.

Gagnaire B., Geffard O., Xuereb B., Margoum C., Garric J. (2008). Choliestersase activities as potential biomarker characterization in two freshwater snails, Potamopyrgus antipodarum (Mollusca, Hydrobiidae, Smith (1889) and Valvata piscinalis (Mollusca, Valvatidae, Mí¼ller 1774). Journal of Chemosphere, 71(3): 553-560.

Geffard O. (2001). Toxicité potentielle des sédiments marins et estuaires contaminés : évaluation chimique et biologique, biodisponibilité des contaminants sédimentaires. Thèse de Doctorat d'Etat en Ecotoxicologie, Université de Bordeaux I (France).

Ghedira J., Jebali J., Bouraoui Z., Banni M., Chouba L., Boussetta H. (2009). A cute effects of chlorpyriphos-ethyl and secondary treated effluents on acetylcholinesterase and butyrylcholinesterase activities in Carcinus maenas. Journal of Environmental Science, 21: 1-6.

Gnassia-Barelli M., Roméo M., Puiseux-dao S. (1995). Effects of cadmium and copper contamination on calcium conten of the bivalve Ruditapes decusstus. Journal of Marine Environmental Research, 35(1-2): 53-57.

Grosell M., Wood C.M., Walsh P.J. (2003). Copper homeostasis and toxicity in the elasmobranch Raja erinacea and the teleost Myoxocephalus octodecemspinosus during exposure to elevated water-borne copper. Journal of Comparative Biochemistry Physiology C Pharmacology Toxicology, 135: 179-190.

Habig C., DiGiulio R. (1991). Biochemical characteristics of cholinesterases in aquatic organisms. In: Mineau, P. (Ed.). Cholinesterase inhibiting insecticides: their impact on wildlife and the environment. Journal of Elsevier Science Publishers, New York. pp: 19-33.

Jebali J., Banni M., Almeida E.A., Bannaoui A., Boussetta H. (2006). Effects of malathion and cadmium on acetylcholinesterase activity and metallothionein levels in the fish Seriola dumerilli. Journal of Fish Physiology Biochemistry, 32: 93-98.

Jebali J., Ben Khedher S., Ghedira J., Kamel N., Boussetta H. (2011). Integrated assessment of biochemical responses in Mediterranean crab (Carcinus maenas) collected from Monastir Bay Tunisia. Journal of Environmental Science, 23(10): 1714–1720.

Jiravanichpaisal P., Miyazaki T. (1994). Histopathology, biochemistry and pathogenecity of Vibrio har6eyi infecting black tiger prawn Penaeus monodon. Journal of Aquatic Animal Health, 6: 27-35.

Johnson P.T. (1980). Histology of the Blue Crab, Callinectes Sapidus. A Model for the Decapoda. Praeger, New York.

Kopecka-Pilarczyk J. (2010). The effect of pesticides and metals on acetylcholinesterase (AChE) in various tissues of blue mussel (Mytilus trossulus L.) in short-term in vivo exposures at different temperatures. Journal of Environmental Science Health B, 45(4): 336-346.

Kumar A., Doan H., Barnes M., Chapman J.C., Kookana R.S. (2010). Response and recovery of acetylcholinesterase activity in freshwater shrimp, Paratya australiensis (Decapoda: Atyidae) exposed to selected anti-cholinesterase insecticides. Journal of Ecotoxicology and Environmental Safety, 73: 1503-1510.

Lane T.W., Morel F.M.M. (2000). A biological function for cadmium in marine diatoms. Journal of Proceeding of the National Academy of Sciences USA, 97: 4627-4631.

Mayer F.L. (1987). Acute toxicity hand book of chemicals to estuarine organisms. US Environmental Protection Agency, Environmental Research Laboratory, Gulf Breeze, FL, EPA/600/8-87/017, April. 274 p.

McHenery J.G., Saward, D., Seaton, D. (1991). Lethal and sub-lethal effects of the salmon delousing agent dichlorvos on the larvae of the lobster (Homarus gammarus L.) and herring (Clupea harengus L.). Journal of Aquaculture, 98: 331-347.

Mora P., Michel X., Narbonne J.F. (1999). Cholinesterase activity as a potential biomarker in two bivalves. Journal of Environmental Toxicology Pharmacology, 7: 253-260.

Olabarrieta I., L'Azou B., Yuric S., Cambar J., Cajaraville M.P. (2001). In vitro effects of cadmium on two different animal cell models. Journal of Toxicology in Vitro, 15(4-5): 511-517.

Pereira P., De Pablo H., Dulce Subida M., Vale C., Pacheco M. (2009). Biochemical responses of the shore crab (Carcinus maenas) in a eutrophic and metal-contaminated coastal system (í“bidos lagoon, Portugal). Journal of Ecotoxicology Environmental Safety, 72: 1471-1480.

Romani R., Antognelli C., Baldracchini F., De Santis A., Isani G., Giovannini E., Rosi G. (2003). Increased acetylcholinesterase activities in specimens of Sparus auratus exposed to sublethal copper concentrations. Journal of Chemico-Biological Interactions, 145: 321-329

Roméo M., Gharbi-Bouraoui S., Gnassia-Barelli M., Dellali M., Aí¯ssa P. (2006). Responses of Hexaplex trunculus to selected pollutants. Journal of Science Total Environmental, 359: 135-144.

Serafim, A., Bebianno, M.J. (2009). Metallothionein role in the kinetic model of copper accumulation and elimination in the clam Ruditapes decussatus. Journal of Environmental Research, 109: 390-399.

Soegianto A., Charmantier-Daures M., Trilles J.P., Charmantier G. (1999a). Impact of copper on the structure of gills and epipodites of the shrimp Penaeus japonicus. Journal of Crustacean Biology, 19: 209-223.

Soegianto A., Charmantier-Daures M., Trilles J.P., Charmantier G. (1999b). Impact of cadmium on the structure of gills and epipodites of the shrimp Penaeus japonicus (Crustacea: Decapoda). Journal of Aquatic Livers Research, 12: 57-70.

Stentiford G.D., Feist S.W. (2005). A histopathological survey of shore crab (Carcinus maenas) and brown shrimp (Crangon crangon) from six estuaries in the United Kingdom. Journal of Invertebrate Pathology, 88: 136-146

Tu H.T., Silvestre F., Scippo M.L., Thome J.P., Phuong N.T., Kestemont P. (2009). Acetylcholinesterase activity as a biomarker of exposure to antibiotics and pesticides in the black tiger shrimp (Penaeus monodon). Journal of Ecotoxicology Environmental Safety 72, 1463-1470.

Turkmen A., Turkmen M. (2005). Seasonal and spatial variations of heavy metals in the spiny rock oyster, Spondylus spinosus, from coastal waters of Iskenderun Bay, Northern East Mediterranean Sea. Journal of Bulletin of Environmental Contamination and Toxicology, 75: 716-722.

Van der Oost R., Beyer J., Vermeulen N.P.E. (2003). Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Journal of Environmental Toxicology Pharmacology, 13, 57-149.

Varó I., Navarro J.C., Amat F., Guilhermino L. (2003). Effect of dichlorvos on cholinesterase activity of the European sea bass (Dicentrarchus labrax). Journal of Pesticide Biochemistry and Physiology, 75: 61-72.

Varó I., Nunes B., Amat F., Torreblanca A., Guilhermino L., Navarro J.C. (2007). Effect of sublethal concentrations of copper sulphate on seabream Sparus aurata fingerlings. Journal of Aquatic Living Resource, 20: 263-270.

Victor B. (1993). Responses of hemocytes and gill tissues to sublethal cadmium chloride poisoning in the crab Paratelphusa hydrodromous (Herbst). Journal of Archives of Environmental Contamination and Toxicology, 24: 432-439.

Victor B. (1993). Gill tissue pathogenicity and hemocyte behaviour in the crab Paratelphusa hydromous exposed to lead chloride. Journal of Environmental Science and health. Part A: Environmental Science and Engineering and Toxicology, 29: 1011-1034.

Vieira L.R., Gravato C., Soares A.M.V.M., Morgado F., Guilhermino L. (2009). Acute effects of copper and mercury on the estuarine fish Pomatoschistus microps: Linking biomarkers to behaviour. Journal of Chemosphere, 76: 1416-1427.

Vioque-Fernández A., de Almeida E.A., Ballesteros J., García-Barrera T., Gómez-Ariza J.L., López-Barea J. (2007). Doñana National Park survey using crayfish (Procambarus clarkii) as bioindicator: esterase inhibition and pollutant levels. Journal of Toxicology Letter, 168: 260-8.

Wagner A., Boman J. (2004). Biomonitoring of trace elements in Vietnamese freshwater mussels. Spectrochimica Acta, 59: 1125-1132.

Waisberg M., Joseph P., Hale B., Beyersmann D. (2003). Molecular and cellular mechanisms of cadmium carcinogenesis. Journal of Toxicology, 192: 95-117.

Zinkl J.G., Lockhart W.L., Kenny S.A., Ward F.J. (1991). The effects of cholinesterase inhibiting insecticides on fish. In: Mineau, P. (Ed.), Chemicals in Agriculture: Cholinesterase Inhibiting Insecticides-Their Impact on wildlife and the Environment. Journal of Elsevier, 2: 233-254.