Implications of white spot syndrome virus disease on DNA integrity, histology and biochemistry of Procambarus clarkii in Egypt
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White spot syndrome virus (WSSV) is a widespread and highly pathogenic virus; that infects shrimp, crayfish and other crustaceans. The objectives of the present study were to investigate WSSV implications on some crayfish tissues, within light and sever stages of infections. Several parameters have been investigated including DNA integrity, oxidative stress, and histological changes in gills, muscles and hepatopancreas cells, using several techniques such as comet assay, DNA fragmentation assay, oxidative stress biomarkers estimation and histopathological examination. Specimens were divided into three groups according to the nested PCR results. Group I included healthy specimens whose tissues were all negative two-step PCR; Group II involved lightly infected specimens with positive two-step PCR. Group III included heavily infected specimens whose tissues were tested mostly positive one-step PCR. WSSV generates an increase in the different parameters of DNA damage (P<0.05) with abnormal histological features and notable reduction (P<0.05) of the endogenous scavengers in the tested tissues of the infected crayfish in comparison with the normal healthy ones. Furthermore, gills were found to be the most affected organ followed by muscle and finally hepatopancreas. These outcomes additionally demonstrated that comet test could proï¬tably be utilized in genotoxicity evaluation protocols in aquatic invertebrates.
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Aebi H. (1984). [13] Catalase in vitro. Methods in Enzymology, 105: 121-126.
Alarifi S., Ali D., Al-Doaiss A.A., Ali B.A., Ahmed M., Al-Khedhairy A.A. (2013). Histologic and apoptotic changes induced by titanium dioxide nanoparticles in the livers of rats. International Journal of Nanomedicine, 8(8): 3937-3943.
Bancroft J., Stevens A., Turner D. (1996). Theory and practice of histological techniques 4th Ed Churchill Living Stone, New York Edinburgh. Madrid, Sanfrancisco.
Chakraborty S., Ghosh U. (2014). White spot syndrome virus (WSSV) in crustaceans: an overview of host-pathogen interaction. Journal of Marine Biology and Oceanography, 31: 2.
Clem R., Hardwick J., Miller L. (1996). Anti-apoptotic genes of baculoviruses. Cell Death and Differentiation, 3(1): 9-16.
Clem R.J., Fechheimer M., Miller L.K. (1991). Prevention of apoptosis by a baculovirus gene during infection of insect cells. Science, 254(5036): 1388-1390.
Danial N.N., Korsmeyer S.J. (2004). Cell death: critical control points. Cell, 116(2): 205-219.
Do T.U., Ho B., Shih S.-J., Vaughan A. (2012). Zinc finger nuclease induced DNA double stranded breaks and rearrangements in MLL. Mutation Research/ Fundamental and Molecular Mechanisms of Mutagenesis, 740(1): 34-42.
Edgerton B.F. (2004). Susceptibility of the Australian freshwater crayfish Cherax destructor albidus to white spot syndrome virus (WSSV). Diseases of Aquatic Organisms, 59: 187-193.
Galli F., Ghibelli L., Buoncristiani U., Bordoni V., D'Intini V., Benedetti S., Canestrari F., Ronco C., Floridi A. (2003). Mononuclear leukocyte apoptosis in haemodialysis patients: the role of cell thiols and vitamin E. Nephrology Dialysis Transplantation, 18(8): 1592-1600.
Garcia C., Damonte E. (2007). Zn finger containing proteins as targets for the control of viral infections. Infectious Disorders-Drug Targets (Formerly Current Drug Targets-Infectious Disorders), 7(3): 204-212.
Hamdi S.A. (2011). Muscle and exoskeleton extracts analysis of both fresh and marine crustaceans Procambarus clarkii and Erugosquilla massavensis. African Journal of Pharmacy and Pharmacology, 5(13): 1589-1597.
Hameed A.S., Yoganandhan K., Sathish S., Rasheed M., Murugan V., Jayaraman K. (2001). White spot syndrome virus (WSSV) in two species of freshwater crabs (Paratelphusa hydrodomous and P. pulvinata). Aquaculture, 201(3): 179-186.
Hayes J.D., Mclellan L.I. (1999). Glutathione and glutathione-dependent enzymes represent a co-ordinately regulated defence against oxidative stress. Free Radical Research, 31(4): 273-300.
Hoffmann J.A., Kafatos F.C., Janeway C.A., Ezekowitz R. (1999). Phylogenetic perspectives in innate immunity. Science, 284(5418): 1313-1318.
Jiravanichpaisal P., Söderhäll K., Söderhäll I. (2004). Effect of water temperature on the immune response and infectivity pattern of white spot syndrome virus (WSSV) in freshwater crayfish. Fish and Shellfish Immunology, 17(3): 265-275.
Kasornchandra J., Boonyaratpalin S., Itami T. (1998). Detection of white-spot syndrome in cultured penaeid shrimp in Asia: Microscopic observation and polymerase chain reaction. Aquaculture, 164(1): 243-251.
Kou G.-H., Peng S.-E., Chiu Y.-L., Lo C.-F. (1998). Tissue distribution of white spot syndrome virus (WSSV) in shrimp and crabs. Advances in Shrimp Biotechnology, 267-271.
Leu J.-H., Lin S.-J., Huang J.-Y., Chen T.-C., Lo C.-F. (2013). A model for apoptotic interaction between white spot syndrome virus and shrimp. Fish and Shellfish Immunology, 34(4): 1011-1017.
Lo C.-F., Ho C.-H., Peng S.-E., Chen C.-H., Hsu H.-C., Chiu Y.-L., Chang C.-F., Liu K.-F., Su M.-S., Wang C.-H. (1996). White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crabs and other arthropods. Diseases of aquatic organisms, 27(3): 215-225.
Lo C.-F., Ho C.-H., Chen C.-H., Liu K., Chiu Y., Yeh P., Peng S., Hsu H., Liu H., Chang C. (1997). Detection and tissue tropism of white spot syndrome baculovirus (WSBV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organs. Diseases of Aquatic Organisms, 30: 53-72.
Madigosky S.R., Alvarez-Hernandez X., Glass J. (1991). Lead, cadmium, and aluminum accumulation in the red swamp crayfish Procambarus clarkii G. collected from roadside drainage ditches in Louisiana. Archives of Environmental Contamination and Toxicology, 20(2): 253-258.
Nishikimi M., Rao N.A., Yagi K. (1972). The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochemical and Biophysical Research Communications, 46(2): 849-854.
OIE (2003). White spot disease. In: OIE (Ed.). OIE manual of diagnostic tests for aquatic animals. OIE, Paris, 285-297
Olive P.L., Banáth J.P., Durand R.E. (1990). Detection of etoposide resistance by measuring DNA damage in individual Chinese hamster cells. Journal of the National Cancer Institute, 82(9): 779-783.
Paglia D.E., Valentine W.N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of Laboratory and Clinical Medicine, 70(1): 158-169.
Ramos-Carreño S., valencia-Yáñez R., Correa-Sandoval F., Ruíz-García N., Díaz-Herrera F., Giffard-Mena I. (2014). White spot syndrome virus (WSSV) infection in shrimp (Litopenaeus vannamei) exposed to low and high salinity. Archives of Virology, 159: 2213-2222.
Richman L.K., Montali R.J., Nichols D.K., Lightner D.V. (1997). A newly recognized fatal baculovirus infection in freshwater crayfish. Proceedings of the American Association of Zoo Veterinarians.
Rodríguez J., Ruiz J., Maldonado M., Echeverría F. (2012). Immunodetection of hemocytes, peneidins and α2"macroglobulin in the lymphoid organ of white spot syndrome virus infected shrimp. Microbiology and Immunology, 56(8): 562-571.
Sablok G., Sánchez-Paz A., Wu X., Ranjan J., Kuo J., Bulla I. (2012). Genome dynamics in three different geographical isolates of white spot syndrome virus (WSSV). Archives of Virology, 157(12): 2357-2362.
Sahtout A.H., Hassan M., Shariff M. (2001). DNA fragmentation, an indicator of apoptosis, in cultured black tiger shrimp Penaeus monodon infected with white spot syndrome virus (WSSV). Diseases of Aquatic Organisms, 44(2): 155-159.
Sanchez-Zazueta E., Martinez-Cordero F.J. (2009). Economic risk assessment of a semi-intensive shrimp farm in Sinaloa, Mexico. Aquaculture Economics and Management, 13(4): 312-327.
Sánchez"Martínez J.G., Aguirre"Guzmán G., Mejía"Ruíz H. (2007). White spot syndrome virus in cultured shrimp: a review. Aquaculture Research, 38(13): 1339-1354.
Shi Z., Wang H., Zhang J., Xie Y., Li L., Chen X., Edgerton B.F., Bonami J.R. (2005). Response of crayfish, Procambarus clarkii, haemocytes infected by white spot syndrome virus. Journal of Fish Diseases, 28(3): 151-156.
Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175(1): 184-191.
Sriram M.I., Kanth S.B.M.; Kalishwaralal K., Gurunathan S. (2010). Antitumor activity of silver nanoparticles in Dalton's lymphoma ascites tumor model. International Journal of Nanomedicine, 5(1): 753-762.
Tan L., Soon S., Lee K., Shariff M., Hassan M., Omar A. (2001). Quantitative analysis of an experimental white spot syndrome virus (WSSV) infection in Penaeus monodon Fabricius using competitive polymerase chain reaction. Journal of Fish Diseases, 24(6): 315-323.
van Hulten M.C., Witteveldt J., Peters S., Kloosterboer N., Tarchini R., Fiers M., Sandbrink H., Lankhorst R.K., Vlak J.M. (2001). The white spot syndrome virus DNA genome sequence. Virology, 286(1): 7-22.
Vlak J.M., Bonami J.R., Flegel T.W., Kou G.H., Lightner D.V., Loh C.F., Walker P.C. (2005). Family Nimaviridae, genus Whispovirus. Virus taxonomy, 8th reportof the International Committee on Taxonomy of Viruses. London, United Kingdom, Elsevier/Academic Press. 1162 p.
Wang S., Zhao X.-F., Wang J.-X. (2009). Molecular cloning and characterization of the translationally controlled tumor protein from Fenneropenaeus chinensis. Molecular Biology Reports, 36(7): 1683-1693.
Wang Y., Hassan M., Shariff M., Zamri S., Chen X. (1999). Histopathology and cytopathology of white spot syndrome virus (WSSV) in cultured Penaeus monodon from peninsular Malaysia with emphasis on pathogenesis and the mechanism of white spot formation. Diseases of Aquatic Organisms, 39(1): 1-11.
Witteveldt J., Vlak J. (2004). Virus-host interactions of white spot syndrome virus. In: K.Y. Leung (Ed.). Current Trends in the Study of Bacterial and Viral Fish and Shrimp Diseases. pp: 237-255.
Zeng Y., Lu C.-P. (2009). Identification of differentially expressed genes in haemocytes of the crayfish (Procambarus clarkii) infected with white spot syndrome virus by suppression subtractive hybridization and cDNA microarrays. Fish and Shellfish Immunology, 26(4): 646-650.
Zhu Y., Gao G. (2008). ZAP-mediated mRNA degradation. RNA Biology, 5(2): 65-67.
Zou H.S., Juan C.C., Chen S.C., Wang H.Y., Lee C.Y. (2003). Dopaminergic regulation of crustacean hyperglycemic hormone and glucose levels in the hemolymph of the crayfish Procambarus clarkii. Journal of Experimental Zoology Part A: Comparative Experimental Biology, 298(1): 44-52.
