Effects of three anesthetics of clove extract, sodium bicarbonate, and lidocaine on blood parameters and cortisol level in male and female broodstocks of Caspian kutum (Rutilus kutum)
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In this study, 32 male and female Caspian kutum were anesthetized with clove extract (50 mg/L), lidocaine (150 mg/L), and sodium bicarbonate (300 mg/L). Blood parameters and serum cortisol levels were investigated in the control (no anesthetic) and the treated groups containing both males and females in 10 min and 24 h after anesthesia. Based on the result, difference in the red blood cells in the control group and treated groups males was not significant. In females, however, the red blood cells in all treated groups was different except lidocaine treatment after both sampling times (P>0.05). The white blood cells of males and females in control group was significantly different in the sodium bicarbonate group in 10 min and 24 hours after anesthesia (P<0.05). The hemoglobin and hematocrit did not change in male and female fish in lidocaine treatment after both times. Cortisol changed in male and female in the sodium bicarbonate group after both times. The WBCs in females was significantly higher in clove extract and lidocaine treatments, whereas RBCs, Hb, Hct, and cortisol were significantly higher in males (P<0.05). Therefore, due to irreversible stressful effects of the sodium bicarbonate, it is not a suitable anesthetic for the studied fish. Although the stressful effects of lidocaine were lower than clove extract, especially in females, the clove extract has no irreversible effects and the stress-induced effects had been moderated 24 h after anesthesia. Hence, lidocaine and then clove extract is recommended as suitable anesthetics, especially for female Caspian kutum.
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Ackerman P.A., Morgan J.D., Iwama G.K. (2005). Anesthetics. CCAC guidelines on: The care and use of fish in research, teaching and testing. Canadian Council on Animal Care, Ottawa CA. 22 p.
Altun T., Bilgin R., DanabaÅŸ D. (2009). Effects of sodium bicarbonate on anaesthesia of common carp (Cyprinus carpio L., 1758) juveniles. Turkish Journal of Fish Aquatic Sciences, 9: 29-31.
Blaxhall P.C., Daisley K.W. (1973). Routine haematological methods for use with fish bloods. Journal of Fish Biology, 5: 771-781.
Booke H.E., Hollender B., Lutterbie G. (1978). Sodium bicarbonate, an inexpensive fish anesthetic for field use. Progressive Fish Culturist, 40(1): 11-13.
Carrasco S., Sumano H., Navohro-Fierro R. (1984).The use of lidocaine-sodium bicarbonate as an anesthetic in fish. Aquaculture, 41: 161-163.
Chen N.H. (2012). Comparison of clinical hematological changes under anesthetization in Crucian carp (Carassius auratus auratus) following treatment with local anesthetics. African Journal of Biotechnology, 11(22): 6149-6142.
Collymore Ch., Tolwani A., Lieggi Ch., Rasmussen S. (2014). Efficacy and safety of 5 anesthetics in adult zebrafish (Danio rerio). Journal of the American Association for Laboratory Animal Science, 53: 198-203.
Effati M., Bahrekazemi M., Saiidi A.A. (2014). Effect of four anesthetics, clove and thyme extracts, lidocaine and sodium bicarbonate on the blood parameters and cortisol amount in silver carp (Hypophthalmichthys molitrix). Reproduction and Aquaculture Sciences Journal, 2: 37-46.
Effati M., Bahrekazemi M. (2017). Effects of four anesthetics, clove extract, thyme extract, lidocaine, and sodium bicarbonate on the blood parameters and cortisol amount in grass carp (Ctenopharyngodon idella). Journal of Marine Biology and Aquaculture, 4: 1-4.
Farahi A., Kasiri M., Sudagar M., Soleimani Iraei M. (2011). Size-relative effectiveness of clove essence as an anesthetic for kutum (Rutilus frisii kutum). Global Veterinaria, 6(2): 180-184.
Farrell A.P., Cech J.J., Richards J.G., Stevens E.D. (2011). Encyclopedia of fish physiology: From genome to environment. Vol 1. Elsevier Inc. USA. 2163 p.
Fish F.F. (1942). The anaesthesia of fish by high carbon dioxide concentrations. Transaction of the American Fisheries Society, 72: 25-29.
Hoseini S.M. (2011). Efficacy of clove powder solution on stress mitigation in juvenile common carps, Cyprinus carpio (Linnaeus). Comparative Clinical Pathology, 20: 359-362.
Hoseini S.M., Hoseini S.A., Nodeh A.J. (2011). Serum biochemical characteristics of Beluga, Huso huso (L.), in response to blood sampling after clove powder solution exposure. Fish Physiology and Biochemistry, 37: 567-572.
Hoseini S.M., Ghelichpour M. (2012). Efï¬cacy of clove solution on blood sampling and hematological study in Beluga, Huso huso. Fish Physiology and Biochemistry, 38: 493-498.
Hoseini S.M., Rajabiesterabad H., Tarkhani R. (2013). Anaesthetic efï¬cacy of eugenol on iridescent shark, Pangasius hypophthalmus (Sauvage, 1878) in different size classes. Aquaculture Research, 46: 405-412.
Houston A.H., Dobric N., Kahurananga R. (1996). The nature of hematological response in fish. Studies on rainbow trout Oncorhynchus mykiss exposed to stimulated winter, spring and summer conditions. Fish Physiology and Biochemistry, 15: 339-347.
Imanpoor M.R., Bagheri T., Hedayati S.S.A. (2010). The anesthetic effects of clove essence in Persian sturgeon, Acipencer persicus. World Journal of Fish and Marine Sciences, 2: 29-36.
Imanpoor M.R., Farahi A. (2011).The effects of different concentrations of clove extract on semen spermatological parameters and hematological characteristics in migrated kutum, Rutilus frisii kutum to Valiabad River. World Journal of Zoology, 6: 149-153.
Keene J.L., Noakes O.L.G., Moccia R.D., Soto C.G. (1998). The efficacy of clove oil as an anesthetic for rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research, 29: 89-101.
Kristan J., Stara A., Turek J., Policar T., Velisek J. (2012). Comparison of the effects of four anesthetics on he¬matological and blood biochemical profiles in pike¬perch (Sander lucioperca L.). Neuro Endocrinology Letters, 33 (3): 66-71.
Lepic P., Stara A., Turek J., Kozak P., Velisek J. (2014). The effects of four anesthetics on hematological and blood biochemical profiles in vimba bream, Vimba vimba. Veterinary Medicine, 59(2): 81-87.
Mazandarani M., Hoseini S.M., Shahriari R. (2015). Anesthesia of wild female Persian sturgeon, Acipenser persicus (Borodin, 1897) breeders during controlled propagation: effects on hematological parameters, stress response and reproductive performance. Journal of Applied Ichthyology, 31: 997-1001.
Mazandarani M., Hoseini S.M. (2016). Menthol and 1, 8-cineole as new anesthetics in common carp, Cyprinus carpio (Linnaeus, 1758). Aquaculture Research, 48: 3041-3051.
Mazandarani M., Hoseini S.M. (2017). Anesthesia of juvenile Persian sturgeon, Acipenser persicus; Borodin 1897, by peppermint, Mentha piperita, extract – Anesthetic efficacy, stress response and behavior. International Journal of Aquatic Biology, 5: 393-400.
Rehulka J. (2000). Influence of astaxanthin on growth rate, condition, and some blood indices of rainbow trout, Oncorhynchus mykiss. Aquaculture, 190: 27-47.
Ross L.G., Ross B. (2008). Anesthetic and sedative techniques for aquatic animals. Third Edition. Wiley publications. 222 p.
Small B.C. (2003). Anesthetic efficacy of metomidate and comparison of plasma cortisol responses to tricaine methane sulfonate, quinaldine and clove oil anesthetized channel catfish Ictalurus punctatus. Aquaculture, 218: 177-185.
Sudagar M., Mohammadi A., Mazandarani M., Pooralimotlagh S. (2009). The Efficacy of clove powder as an anesthetic and its effects on hematological parameters on roach (Rutilus rutilus). Journal of Aquatic Feed Sciences Nutrition, 1: 1-5.
Taheri Mirghaed A., Ghelichpour M., Hoseini S.M. (2016). Myrcene and linalool as new anesthetic and sedative agents in common carp, Cyprinus carpio - Comparison with eugenol. Aquaculture, 464: 165-170.
Velisek J., Svobodova Z., Piackova V., Groch L., Nepejchalova L. (2005). Effects of clove oil anesthesia on common carp (Cyprinus carpio L.). Veterinary Medicine, 50: 269-274.
Velisek J., Wlasow T., Gomulka P., Svobodova Z., Novotny L., Ziomek E. (2006). Effects of clove oil anesthesia on European catfish (Silurus glanis L.). Acta Veterinaria Brno, 75: 99-106.
Velisek J., Stara A., Li Z., Silovska S., Turek J. (2011). Com¬parison of the effects of four anesthetics on blood biochemical profiles and oxidative stress biomarkers in rainbow trout. Aquaculture, 310: 369-375.
Wagner E., Arndt R., Hilton B. (2002). Physiological stress responses, egg survival and sperm motility for rainbow trout broodstock anesthetized with clove oil, tricaine methanesulfonate or carbon dioxide. Aquaculture, 211: 353-366.
Wedemeyer G.A., Barton B.A., Mcleay D.J. (1990). Stress and acclimation. In: C.B. Schreck, P.B. Moyle (Eds.). Methods for fish biology, Bethesda, Maryland: American Fisheries Society. pp: 451-489.
Wendelaar Bonga S.E. (1997). The stress response in fish. Physiological Reviews, 77: 591-625.
Yaghobi M., Paykan Heyrati F., Dorafshan S., Mahmoudi N. (2015). Serum biochemical changes and acute stress responses of the endangered iridescent catfish (Pangasianodon hypophthalmus) supplied with dietary nucleotide. Journal of Agriculture Sciences and Technology, 17: 1161-1170.
Yousefi M., Hoseinifar H., Ghelichpour M., Hoseini S.M. (2018). Anesthetic efficacy and biochemical effects of citronellal and linalool in common carp (Cyprinus carpio Linnaeus, 1758) juveniles. Aquaculture, 493: 107-112.
