Caspian Sea’s Navicula salinicola Hustedt 1939 and effect of the prolonged culture on its fatty acid profile

Ehsan Etesami, Farkhondeh Saba, Mostafa Noroozi, Mohammad Ali Amoozegar, Gholamreza Bakhshi Khaniki, Seyed Abolhassan Shahzadeh Fazeli

Abstract

Diatoms are a potent source of polyunsaturated fatty acids. This study was conducted for screening a Naviculoid diatom strains from the southern Caspian Sea with analyzing its lipid production and accumulation potentials. The isolate was identified as Navicula salinicola strain IBRC-M 5083 based on micro-morphological characterization and analysis of 18S rRNA genomic region. Navicula salinicola were cultured in the f/2 medium under both normal and prolonged culture (21 days) conditions. Total lipid percentages of this strain were found to be 31.83% under normal condition and 43.72±1.4% in prolonged culture respectively on the basis of their dry cell weight (DCW). Also, the oil droplets were detected in 21 days’ cells as shown by Sudan Black B staining experiments. Furthermore, the main fatty acids were found by Gas Chromatography analyses of this strain under prolonged condition to be Eicosapentaenoic acid (25.58%TFA). Such oil accumulation capabilities seem to be promising for performing further studies on this strain as a source of Omega-3 in aquafeed, pharmaceutical and biofuel industries.

Keywords

Algae, Omega3, Eicosapentaenoic acid, Docosahexaenoic acid.

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References

Axelsson M., Gentili F. (2014). A single-step method for rapid extraction of total lipids from green microalgae. PLoS One, 9(2): e89643.

Brett M., Muller-Navarra D.O. (1997). The role of highly unsaturated fatty acids in aquatic food web processes. Freshwater Biology, 38(3): 483-499.

Burdon K.L. (1946). Fatty material in bacteria and fungi revealed by staining dried, fixed slide preparations. Journal of Bacteriology, 52(6): 665.

Cavonius L.R., Carlsson N.G., Undeland I. (2014). Quantification of total fatty acids in microalgae: comparison of extraction and transesterification methods. Analytical and Bioanalytical Chemistry, 406(28): 7313-7322.

Cox E.J. (1990). Studies on the algae of a small softwater stream II. Algal standing crop (measured by chlorophyll-a) on soft and hard substrata. Archiv für Hydrobiologie (Supplement), 83(4): 553-566.

Crawford S.A., Higgins M.J., Mulvaney P., Wetherbee R. (2001). Nanostructure of the diatom frustule as revealed by atomic force and scanning electron microscopy. Journal of Phycology, 37(4): 543-554.

Dawson S.C., Pace N.R. (2002). Novel kingdom-level eukaryotic diversity in anoxic environments. Proceedings of the National Academy of Sciences, 99(12): 8324-8329.

D’Ippolito G., Sardo A., Paris D., Vella F.M., Adelfi M.G., Botte P., Fontana A. (2015). Potential of lipid metabolism in marine diatoms for biofuel production. Biotechnology for Biofuels, 8(1): 1.

Dunahay T., Benemann J., Roessler P. (1998). A look back at the US department of energy's aquatic species program: biodiesel from algae (Vol. 328). Golden: National Renewable Energy Laboratory.

Edgar S. M., Theriot E.C. (2004). Phylogeny of Aulacoseira (Bacillariophyta) based on molecules and morphology. Journal of Phycology, 40(4), 772-788.

Folch J., Lees M., Sloane-Stanley G.H. (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, 226(1): 497-509.

Graham J.M., Graham L.E., Zulkifly S.B., Pfleger B.F., Hoover S.W., Yoshitani J. (2012). Freshwater diatoms as a source of lipids for biofuels. Journal of Industrial Microbiology and Biotechnology, 39(3): 419-428.

Griffiths M.J, Harrison S.T.L (2009). Lipid productivity as a key characteristic for choosing algal species for biodiesel production. Journal of Applied Phycology 21: 493-507.

Guillard R.R.L. (1975). Culture of phytoplankton for feeding marine invertebrates. In: W.L. Smith, M.H. Chanley (Eeds). Culture of Marine Invertebrate Animals. New York: Plenum Press, p. 26e60.

Hildebrand M., Davis A.K., Smith S.R., Traller J.C., Abbriano R. (2012). The place of diatoms in the biofuels industry. Biofuels, 3(2): 221-240.

Hu Q., Sommerfeld M., Jarvis E., Ghirardi M., Posewitz M., Seibert M., Darzins A. (2008). Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. The Plant Journal, 54(4): 621-639.

Jape A., Harsulkar A., Sapre V. R. (2014). Modified Sudan Black B staining method for rapid screening of oleaginous marine yeasts. International Journal of Current Microbiology and Applied Sciences, 3(9): 41-46.

Juneja A., Ceballos R.M., Murthy G.S. (2013). Effects of environmental factors and nutrient availability on the biochemical composition of algae for biofuels production: a review. Energies, 6(9): 4607-4638.

Kitano M., Matsukawa, R., Karube I. (1997). Changes in eicosapentaenoic acid content of Navicula saprophila, Rhodomonas salina and Nitzschia sp. under mixotrophic conditions. Journal of applied phycology, 9(6), 559-563.

Khatoon H. (2006). Use of selected periphyton species to improve the water quality and shrimp post larval production (Doctoral dissertation, Universiti Putra Malaysia).

Kociolek J.P. (2005). A checklist and preliminary bibliography of the Recent, freshwater diatoms of inland environments of the continental United States. Proceedings of the California Academy of Sciences. Fourth Series, 56(27): 395-525.

Kociolek J.P., Stepanek J.G., Lowe R.L., Johansen J.R., Sherwood A.R. (2013). Molecular data show the enigmatic cave-dwelling diatom Diprora (Bacillariophyceae) to be a raphid diatom. European Journal of Phycology, 48(4): 474-484.

Lison L. (1934). Sur des nouveaux colorants histologiques spécifiques des lipides. Comptes Rendus des Seances de la Societe de Biologie et des ses Filiales (Paris), 115: 202-205.

Liu D., Coloe S., Baird R., Pedersen J. (2000). Rapid mini-preparation of fungal DNA for PCR. Journal of Clinical Microbiology, 38(1): 471-471.

Matsumoto M., Sugiyama H., Maeda Y., Sato R., Tanaka T., Matsunaga T. (2010). Marine diatom, Navicula sp. strain JPCC DA0580 and marine green alga, Chlorella sp. strain NKG400014 as potential sources for biodiesel production. Applied biochemistry and biotechnology, 161(1-8): 483-490.

Medlin L., Elwood H.J., Stickel S., Sogin M.L. (1988). The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene, 71(2): 491-499.

Mekhalfi M., Amara S., Robert S., Carrière F., Gontero B. (2014). Effect of environmental conditions on various enzyme activities and triacylglycerol contents in cultures of the freshwater diatom, Asterionella formosa (Bacillariophyceae). Biochimie, 101: 21-30.

Merz C.R., Main K.L. (2014). Microalgae (diatom) production the aquaculture and biofuel nexus. In Oceans-St. John's, IEEE. pp: 1-10.

Nagle N., Lemke P. (1990). Production of methyl ester fuel from microalgae. Applied Biochemistry and Biotechnology, 24(1): 355-361.

Preisig H.R., Andersen R.A. (2005). Historical review of algal culturing techniques. Algal Culturing Techniques, 65: 79-82.

Ravikumar K., Dakshayini J., Girisha S.T. (2012). Biodiesel production from oleaginous fungi. International Journal of Life Sciences, 6(1): 43-49.

Round F.E., Crawford R.M., Mann D.G. (1990). Diatoms: biology and morphology of the genera. Cambridge University Press. 747 p.

Simopoulos A.P. (1991). Omega-3 fatty acids in health and disease and in growth and development. The American Journal of Clinical Nutrition, 54(3): 438-463.

Sivakumar G., Vail D.R., Xu J., Burner D.M., Lay J.O., Ge X., Weathers P.J. (2010). Bioethanol and biodiesel: Alternative liquid fuels for future generations. Engineering in Life Sciences, 10(1): 8-18.

Sheehan J., Dunahay T., Benemann J., Roessler P. (1998). A look back at the U.S. Department of Energy’s Aquatic Species Program: Biodiesel from Algae. Close-out report. National Renewable Energy Lab, Department of Energy, Golden, Colorado, U.S.A. Report number NREL/TP-580-24190, dated July 1998.

Stoermer E.F., Kreis R.G. Jr., Andresen N.A. (1999). Checklist of Diatoms from the Laurentian Great Lakes. II. Journal of Great Lakes Research 25(3): 515-566.

Thajuddin N., Ilavarasi A., Baldev E., MubarakAli D., Alharbi N.S., Chinnathambi A., Alharbi S.A. (2015). Stress induced lipids accumulation in Naviculoid marine diatoms for bioenergy application. International Journal of Biotechnology for Wellness Industries, 4(1): 18-24.

Thakur M.S., Prapulla S.G., Karanth N.G. (1989). Estimation of intracellular lipids by the measurement of absorbance of yeast cells stained with Sudan Black B. Enzyme and Microbial Technology, 11: 252-254.

Yoneda K., Yoshida M., Suzuki I., Watanabe M.M. (2016). Identification of a major lipid droplet protein in a marine diatom Phaeodactylum tricornutum. Plant and Cell Physiology, 57(2): 397-406

Yongmanitchai W., Ward O.P. (1991). Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions. Applied and Environmental Microbiology, 57(2): 419-425.

Zheng W., Wang X., Wang Y., Chu C. (2007). Effects of different nutritional minerals on the growth of Navicula BT001. Oceanologia ET Limnologia Sinica, 38(2): 157.

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