Histopathological study of common carp (Cyprinus carpio) fed aflatoxin-contaminated diets
This study aimed to evaluate the effects of aflatoxin-contaminated diet on histopathological alterations of the gill, liver, kidney and intestine tissues in common carp. Fish were randomly distributed into 15 tanks, i.e. in five experimental groups; (I) control fed with normal diet without solvent and aflatoxin, (II) positive control received feed with only solvent, and (III-V) fed on diets containing 0.5, 0.7 and 1.4 mg kg-1 of aflatoxin, respectively. After 21-days, 12 fish per treatment were randomly caught, anesthetized and euthanized. Then, histological sections of the tissues were prepared. The main aflatoxicosis symptoms in the gills were fusion and disorganisation of the secondary gill lamellae, shortening of the secondary lamellae, inflammation of mucous membranes, and exfoliation of the gill epithelium. Liver of the infected fish indicated cloudy swelling of hepatocytes, cellular hypertrophy, formation of vacuoles in the cytoplasm, and necrosis of liver parenchyma. Expansion of Bowman's space, necrosis of urinary tract, exfoliation and degeneration of the urinary tract epithelium, expansion of the urinary lumen and dilation of the urinary space were observed symptoms in the kidney. Changes in the intestine of the aflatoxin-treated fish were; expansion of goblet cells, necrosis of mucous layers, exfoliation of the mucous epithelium, and bleeding in the intestinal wall. The results indicates that feeding common carp with diets contaminated with aflatoxin, even in low concentrations (â‰¤ 1.4 mg kg-1 feed) can cause histopathological damages and disturb their physiological balance.
Adedara I.A., Owumi S.E., Uwaifo A.O., Farombi E.O. (2010). Aflatoxin B1 and ethanol co-exposure induces hepatic oxidative damage in mice. Toxicology and Industrial Health, 26: 717-724.
Almeida I.F.M., Martins H.M.L., Santos S.M.O., Freitas M.S., da Costa, J.M.G.N., d'Almeida Bernardo F.M. (2011). Mycobiota and aflatoxin B1 in feed for farmed sea bass (Dicentrarchus labrax). Toxins, 3: 163-171.
Applegate T.J., Schatzmayer G., Pricket K., Troche C., Jiang Z. (2009). Effect of aflatoxin culture on intestinal function and nutrient loss in laying hens. Poultry Science, 88(6): 1235-1241.
Arafa M.M., Ali A.T., Mohammed S.F. (2014). Detecting the effect of Aflatoxin B1 polluted rations on freshwater fish, Lates niloticus reared in fish cultured ponds. Current Science International, 3(1): 34-39.
Arana S., Alves V.A.F., Sabino M., Tabata Y.A., Nonogaki S., Zaidan-Dagli M.L., Hernandez-Blazquez F.J. (2014). Immunohistochemical evidence for myofibro-blast-like cells associated with liver injury induced by aflatoxin B1 in rainbow trout (Oncorhynchus mykiss). Journal of Comparative Pathology, 150(2): 258-265.
Banaee M., Sureda A., Mirvagefei A.R. Ahmadi K. (2013). Histopathological alterations induced by diazinon in rainbow Trout (Oncorhynchus mykiss). International Journal of Environmental Research, 7(3): 735-744.
Bernabucci U., Colavecchia L., Danieli P.P., Basiricò L., Lacetera N., Nardone, A., Ronchi B. (2011). Aflatoxin B1 and fumonisin B1 affect the oxidative status of bovine peripheral blood mononuclear cells. Toxicology in Vitro, 25(3): 684-691.
Chávez-Sánchez M.C., Martínez Palacios C.A., Moreno I.O. (1994). Pathological effects of feeding young Oreochromis niloticus diets supplemented with different levels of aflatoxin B1. Aquaculture, 127(1): 49-60.
Chawanthayatham S., Thiantanawat A., Egner P.A., Groopman J.D., Wogan G.N., Croy R.G., Essigmann J.M. (2015). Prenatal exposure of mice to the human liver carcinogen Aflatoxin B1 reveals a critical window of susceptibility to genetic change. International Journal of Cancer, 136(6): 1254-1262.
Deng S.X., Tian L.X., Liu F.J., Jin S.J., Liang G.Y., Yang H.J., Du Z.Y., Liu Y.J. (2010). Toxic effects and residue of aflatoxin B1 in tilapia (Oreochromis niloticus í— O. aureus) during long-term dietary exposure. Aquaculture, 307(3): 233-240.
Denli M., Blandon J.C., Guynot M.E., Salado S., Perez J.F. (2009). Effects of dietary AflaDetox on performance, serum biochemistry, histopathological changes, and aflatoxin residues in broilers exposed to aflatoxin B1. Poultry Science, 88(7): 1444-1451.
Eagderi S., Mojazi Amiri B., Adriaens D. (2013). Description of the ovarian follicle maturation of the migratory adult female bulatmai barbel (Luciobarbus capito, Gí¼ldenstädt 1772) in captivity. Iranian Journal of Fisheries Sciences, 12(3): 550-560.
El-Greisy Z.A., El-Gamal A.H.A. (2015). Experimental studies on the effect of cadmium chloride, zinc acetate, their mixture and the mitigation with vitamin C supplementation on hatchability, size and quality of newly hatched larvae of common carp, Cyprinus carpio. Egyptian Journal of Aquatic Research, 41: 219-226.
El-Sayed Y.S., Khalil R.H. (2009). Toxicity, biochemical effects and residue of aflatoxin B1, in marine water-reared sea bass (Diacentrarchus labrax L.). Food and Chemical Toxicology, 47(7): 1606-1609.
Huang Y., Han D., Zhu X., Yang Y., Jin J., Chen Y., Xie S. (2011). Response and recovery of gibel carp from subchronic oral administration of aflatoxin B1. Aquaculture, 319(2): 89-97.
Kensler T.W., Roebuck B.D., Wogan G.N., Groopman J.D. (2011). Aflatoxin: a 50-year odyssey of mechanistic and translational toxicology. Toxicological Sciences, 120(1): 28-48.
Mahfouz, M.E. (2015). Ameliorative effect of curcumin on aflatoxin B1-induced changes in liver gene expression of Oreochromis niloticus. Molecular Biology, 49(2): 275-286.
Mahfouz M.E., Sherif H.A. (2015). A multiparameter investigation into adverse eï¬€ects of aï¬‚atoxin on Oreochromis niloticus health status. The Journal of Basic and Applied Zoology, 71: 48-59.
Mary V.S., Theumer M.G., Arias S.L., Rubinstein H.R. (2012). Reactive oxygen species sources and biomolecular oxidative damage induced by aflatoxin B1 and fumonisin B1 in rat spleen mononuclear cells. Toxicology, 302(3): 299-307.
Matur E., Ergul E., Akyazi I., Eraslan E., Inal G., Bilgic S., Demircan H. (2011). Effects of Saccharomyces cerevisiae extract on haematological parameters, immune function and the antioxidant defense system in breeder hens fed aflatoxin contaminated diets. British Poultry Science, 52(5): 541-550.
McKean C., Tang L., Tang M., Billam M., Wang Z., Theodorakis C.W., Kendall R.J., Wang J.S. (2006). Comparative acute and combinative toxicity of aflatoxin B1 and fumonisin B1 in animals and human cells. Food and Chemical Toxicology, 44(6): 868-876.
Mehrim A.I., Abdelhamid A.M., Abo Shosha A.A.M., Salem M.F.I., El-Sharawy M.A.M.M. (2006). Nutrtious attempts to detoxify aflatoxic diets of tilapia fish 2-clinical, biochemical and histological parameters. Journal of the Arabian Aquaculture Society, 1(2): 69-90.
Mobasher M., Aramesh K., Aldavoud S., Ashrafganjooei N., Divsalar K., Phillips C., Larijani B. (2008). Proposing a national ethical framework for animal research in Iran. Iranian Journal of Public Health, 37(1): 39-46.
Mohapatra S., Sahu N.P., Pal A.K., Prusty A.K., Kumar V., Kumar S. (2011). Haemato-immunology and histo-architectural changes in Labeo rohita fingerlings: effect of dietary aflatoxin and mould inhibitor. Fish Physiology and Biochemistry, 37(1): 177-186.
Raghavan P.R., Zhu X., Lei W., Han D., Yang Y., Xie S. (2011). Low levels of aflatoxin B1 could cause mortalities in juvenile hybrid sturgeon, Acipenser ruthenus â™‚ í— A. baeri. Aquaculture Nutrition, 17(2): 39-47.
Roberts R.J. (2001). Fish pathology, 3rd ed. Edinburgh, England: W.B. Saunders, Harcornt publishers.
Ruby D.S., Masood A., Fatmi A. (2013). Effect of aflatoxin contaminated feed on growth and survival of fish Labeo rohita (Hamilton). Current World Environment, 8(3): 479-482.
Russo J.R., Yanong R.P.E. (2010). Molds in fish feeds and aflatoxicosis. Institute of Food and Agriculture Sciences, University of Florida; Gainesville, FL, USA.
Sahoo P.K., Mukherjee S.C., Jain A.K., Mukherjee A. (2003). Histopathological and electron microscopic studies of gills and opisthonephros of rohu, Labeo rohita, to acute and subchronic aflatoxin B1 toxicity. Asian Fisheries Science, 16: 257-268.
Sahoo P.K., Mukherjee S.C., Nayak S.K., Dey S. (2001). Acute and subchronic toxicity of aflatoxin B1 to rohu, Labeo rohita (Hamilton). Indian Journal of Experimental Biology, 39(5): 453-458.
Shotwell O.L., Hesseltine C.W., Stubblefield R.D., Sorenson W.A. (1996). Production of aflatoxin on rice. Journal of Applied Microbiology, 14: 425-428.
Singh R.N. (2012). Histopathological alterations in the kidney of Cyprinus carpio after exposure to dimethoate (EC 30%). Indian Journal of Science Research, 3(1): 127-131.
Spring P., Fegan D.F. (2010). Mycotoxins - A rising threat to aquaculture, Brentwood, TN, USA: Alltech Inc.
Taheri S., Banaee M., Nematdoost Haghi B., Mohiseni M. (2017). Evaluation of nephrotoxic effects of aflatoxins on common carp (Cyprinus carpio). Iranian Journal of Toxicology, 11(2): 51-58.
Taheri T., Semnani S., Roshandel G., Namjoo M., Keshavarzian H., Chogan A.G., Ghasemi Kebria F., Joshaghani H. (2012). Aflatoxin Contamination in Wheat Flour Samples from Golestan Province, Northeast of Iran. Iranian Journal of Public Health, 41(9): 42-47.
Tuan N.A., Grizzle J.M., Lovell R.T., Manning B.B., Rottinghaus E.G. (2002). Growth and hepatic lesions of Nile tilapia Oreochromis niloticus fed diets containing aflatoxin B1. Aquaculture, 212(4): 311-319.
Varior S. (2003). Biochemical and Histopathological effects of aflatoxin on Oreochromis mossambicus (Peters). Ph.D Thesis, Faculty of Marine Sicence, Cochin University of Science and Technology, Kochi, India. 209 p.
Vaziryan M., Banaee M., Nematdoost Hagi B., Mohiseni M. (2017). Effects of dietary exposure to aflatoxins on some plasma biochemical parameters of common carp (Cyprinus carpio). Iranian Journal of Fisheries Sciences, (In Press)
Wacoo A.P., Wendiro D., Vuzi P.C., Hawumba J.F. (2014). Methods for detection of aflatoxins in agricultural food crops. Journal of Applied Chemistry, 706291: 1-15.
Zychowski K.E., Hoffmann A.R., Ly H.J., Pohlenz C., Buentello A., Romoser A., Gatlin D.M., Phillips T.D. (2013). The effect of aflatoxin-B1 on Red Drum (Sciaenops ocellatus) and assessment of dietary supplementation of NovaSil for the prevention of aflatoxicosis. Toxins, 5(9): 1555-1573.