Influence of probiotic, Lactobacillus plantarum on serum biochemical and immune parameters in vaccinated rainbow trout (Oncorhynchus mykiss) against streptococcosis/lactococosis

Ali. M. Kane; Mehdi Soltani; Hossein Ali Ebrahimzahe-Mousavi; Komeil Pakzad

doi:10.22034/ijab.v4i4.226

Authors

Ali. M. Kane Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Mehdi Soltani
[email protected]
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Hossein Ali Ebrahimzahe-Mousavi Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Komeil Pakzad Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Vol. 4 No. 4 (2016): August

Articles

October 10, 2016

Downloads

PDF

Abstract
How to Cite
Metrics
References

This study evaluated the effects of probiotic, Lactobacillus plantarum on serum biochemical and some immune parameters of immunized rainbow trout weighting 29.6±1.84 g, with streptococcosis/lactococosis vaccine at 16±1.5°C, for 60 days. A commercial diet was used as the control. Fish in the first treatment were immunized with streptococcosis/lactococosis vaccine in bathing route for 1 min. In the second group, the vaccinated trout were also fed diet containing L. plantarum (10⁸ CFU g^-1). In the third treatment, fish were only fed the diet supplemented with L. plantarum (10⁸ CFU g^-1). The results showed that vaccinated trout with or without L. plantarum feeding diets significantly decreased heterophils. Meanwhile it enhances serum lysozyme, alternative complement activities, antibody titer, total leukocytes, lymphocytes, and serum biochemical parameters, including ALP, IgM, and total protein levels compared to control groups. Moreover, the highest levels of above mentioned parameters were found in vaccinated fish that fed L. plantarum. In addition, the vaccinated fish that fed L. plantarum showed significantly elevated cholesterol levels compared to the control group. The results showed that the dietary L. plantarum improved the immunity of immunized trout with streptococcosis/lactococosis vaccine.

Kane, A. M., Soltani, M., Ebrahimzahe-Mousavi, H. A., & Pakzad, K. (2016). Influence of probiotic, Lactobacillus plantarum on serum biochemical and immune parameters in vaccinated rainbow trout (Oncorhynchus mykiss) against streptococcosis/lactococosis. International Journal of Aquatic Biology, 4(4), 285–294. https://doi.org/10.22034/ijab.v4i4.226

Download Citation

Allen F.M., Patrick J.W., Roger T.H. (2005). Blood biochemistry of the oyster toadfish. Journal of Aquatic Animal Health, 17: 170-176.

Austin B., Austin D.A. (2007). Bacterial fish pathogens: disease of farmed and wild fish. 3th Edn. Springer Praxis, Godalming, UK. 457 p.

Bachrach G., Zlotkin A., Hurvitz A., Evans D.L., Eldar A. (2001). Recovery of Streptococcus iniae from diseased fish previously vaccinated with a streptococcus vaccine. Applied and Environmental Microbiology, 67: 3756-3758.

Buchanan J.T., Stannard J.A., Lauth X., Ostland V.E., Powell H.C., Westerman M.E., Nizet V. (2005). Streptococcus iniae phosphoglucomutase is a virulence factor and a target for vaccine development. Infection and Immunity, 73: 6935-6944.

Cebeci A., Gurakan C. (2003). Properties of potential probiotic Lactobacillus plantarum strains. Food Microbiology, 20: 511-518.

Cheng S., Hu Y.H., Jiao X.D., Sun L. (2010). Identification and immunoprotective analysis of a Streptococcus iniae subunit vaccine candidate. Vaccine, 28: 2636-2641.

Cruz P.M., Ibáñez A.L., Hermosillo O.A.M., Saad H.C.R. (2012). Use of probiotics in aquaculture. International Scholarly Research Network Microbiology. doi: 10.5402/2012/916845.

Eguchi M. (1995). Alkaline phosphatase isozymes in insects and comparison with mammalian enzyme. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 111: 151-162.

Ellis A.E. (1990). Lysozyme assays. In: J.S. Stolen, T.C. Fletcher, D.P. Anderson, B.S. Roberson, W.B. van Muiswinkel (Eds.). Techniques in Fish Immunology. SOS Publications. New Jersey, USA. pp: 101-113.

Evans J.J., Klesius P.H., Shoemaker C.A. (2004). Efficacy of Streptococcus agalactiae (Group B) vaccine in tilapia (Oreochromis niloticus) by intraperitoneal and bath immersion administration. Vaccine, 22: 3769-3773.

Eyngor M., Tekoah Y., Shapira R., Hurvitz A., Zlotkin A., Lublin A., Eldar A. (2008). Emergence of novel Streptococcus iniae exopolysaccharide-producing strains following vaccination with nonproducing strains. Applied and Environmental Microbiology, 74: 6892-6897.

Ghosh S., Sinha A., Sahu C. (2008). Dietary probiotic supplementation in growth and health of live-bearing ornamental fishes. Aquaculture Nutrition, 14: 298-299.

Giri S.S., Sukumaran V., Oviya M. (2013). Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita. Fish and Shellfish Immunology, 34: 660-666.

Giri S.S., Sukumaran V., Sen S.S., Jena P.K. (2014). Effects of dietary supplementation of potential probiotic Bacillus subtilis VSG1 singularly or in combination with Lactobacillus plantarum VSG3 or/and Pseudomonas aeruginosa VSG2 on the growth, immunity and disease resistance of Labeo rohita. Aquaculture Nutrition, 20: 163-171.

Haghighi Karsidani S., Soltani M., Nikbakhat-Brojeni G., Ghasemi M., Skall H.F. (2010). Molecular epidemiology of zoonotic streptococcosis/ lactococcosis in rainbow trout (Oncorhynchus mykiss) aquaculture in Iran. Iran Journal of Microbiology, 2: 198-209.

Hastein T., Gudding R., Evensen O. (2005). Bacterial vaccines for fish-an update of the current situation worldwide. Developments in Biologicals (Basel), 121: 55-74.

Kim D.H., Austin B. (2006). Innate immune responses in rainbow trout (Oncorhynchus mykiss, Walbaum) induced by probiotics. Fish and Shellfish Immunology, 21: 513-524.

Klontz G.W. (1994). Fish hematology. In: J.S. Stolen, T.C. Fletcher, A.F. Rowley, T.C. Kelikoff, S.L. Kaattari, S.A. Smith (Eds.). Techniques in fish immunology. Vol 3, SOS Publications, Fair Haven, New Jersey, USA. pp: 121-132.

Misra C.K., Das B.K., Mukherjee S.C., Meher P.K. (2006). The immunomodulatory effects of tuftsin on the non-specific immune system of Indian Major carp, Labeo rohita. Fish and Shellfish Immunology, 20: 728-738.

Newaj-Fyzul A., Austin B. (2015). Probiotics, immunostimulants, plant products and oral vaccines, and their role as feed supplements in the control of bacterial fish diseases. Journal of Fish Diseases, 38: 937-955.

Panigrahi A., Kiron V., Kobayashi T., Puangkaew J., Satoh S., Sugita H. (2005). The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout Oncorhynchus mykiss. Aquaculture, 243: 241-253.

Pridgeon J.W., Klesius P.H. (2012). Major bacterial diseases in aquaculture and their vaccine development. CAB Reviews, 7(48). doi: 10.1079/PAVSNNR20127048.

Pridgeon J.W., Klesius P.H. (2011). Development and efficacy of a novobiocin-resistant Streptococcus iniae as a novel vaccine in Nile tilapia (Oreochromis niloticus). Vaccine, 29: 5986-5993.

Ridha M.T., Azad I.S. (2012). Preliminary evaluation of growth performance and immune response of Nile tilapia Oreochromis niloticus supplemented with two putative probiotic bacteria. Aquaculture Research, 43: 843-852.

Roberson B.S. (1990). Bacterial agglutination. In: J.S. Stolen, T.C. Fletcher, D.P. Anderson, B.S. Roberson, W.B. van Muiswinkel (Eds.). Techniques in fish immunology. SOS Publications, New Haven, New Jersey, USA. pp: 81-86.

Sharifuzzaman S.M., Austin B. (2010a). Kocuria SM1controls vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum). Journal of Applied Microbiology, 108: 2162-2170.

Sharifuzzaman S.M., Austin B. (2010b). Development of protection in rainbow trout (Oncorhynchus mykiss, Walbaum) to Vibrio anguillarum following use of the probiotic Kocuria SM1. Fish and Shellfish Immunology, 29: 212-216.

Soltani M., Jamshidi S, Sharifpour I. (2005). Streptococcosis caused by Streptococcus iniaein farmed rainbow trout (O. mykiss) in Iran: Biophysical characteristics and pathogenesis. Bulletin- European Association of Fish Pathologists, 25: 95-106.

Soltani M., Alishahi M., Mirzargar S., Nikbakht G. (2007). Vaccination of rainbow trout against Streptococcus iniae infection: comparison of different routes of administration and different vaccines. Iranian Journal of Fisheries Sciences, 7: 129-40.

Sommerset I., Krossí¸y B., Biering E., Frost P. (2005). Vaccines for fish in aquaculture. Expert Review of Vaccines, 4: 89-101.

Son V.M., Chang C.C., Wu M.C., Guu Y.K., Chiu C.H., Cheng W. (2009). Dietary administration of the probiotic, Lactobacillus plantarum, enhanced the growth, innate immune responses, and disease resistance of the grouper Epinephelus coioides. Fish and Shellfish Immunology, 26: 691-698.

Sun Y., Sun L., Xing M-Q., Liu C.S., Hu Y.H. (2013). SagE induces highly effective protective immunity against Streptococcus iniae mainly through an immunogenic domain in the extracellular region. Acta Veterinaria Scandinavica, 12: 55-78.

Sun Y.Z., Yang H.L., Ma R.L., Lin W.Y. (2010). Probiotic applications of two dominant gut Bacillus strains with antagonistic activity improved the growth performance and immune responses of grouper Epinephelus coioides. Fish and Shellfish Immunology, 29: 803-809.

Zou L., Wang J., Huang B., Xie M., Li A., Mts B. (2011). A hydrophobic membrane protein of Streptococcus iniae, is an effective subunit vaccine candidate. Vaccine, 29: 391-394.