Effect of propolis on gene expiration IL-1? in Cyprinus carpio challenged with Aeromonas hydrophilia
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Aquaculture has become a significant source of income in areas facing declining natural fishery productivity. This work aimed to investigate the effect of Water Ethanol Extract propolis (WEEP) on peritoneal macrophages in vitro production of cytokines, including interleukin-1beta (IL-1?) in common carp, Cyprinus carpio challenged with Aeromonas hydrophilia. The results of hematological parameters revealed substantial alterations, particularly significant in the 2g/kg propolis group (T2), where red blood cells, packed cell volume, and hemoglobin exhibited changes, indicating potential advantages for fish health by intriguing immunomodulatory effects. The 2 g/kg propolis group (T2) increased WBC count, suggesting propolis's potential as an immunostimulant. Interleukin-1? (IL-1?) gene expression results showed a significant decrease in T2 compared to T3. This finding revealed the intricate relationship between propolis concentrations and the modulation of the immune response. The results showed the positive effect of propolis on as potential application as an immunostimulant in aquaculture.
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Acharya G., Mohanty P.K. (2018). Effect of sex on haemocytobiochemical profiling of silver tiger fish (Datnioides jpolota Hamilton, 1822). Comparative Clinical Pathology, 27(5): 1335-1342.
Alwash S.W., Al-Faragi J.K., Al-Saadi T.M. (2022). Efficiency of copper nanoparticles coated mint as anti-fungal against saprolegniasis disease in common carp. Iraqi Journal of Agricultural Sciences, 53(5): 1129-1137.?
Anjum S.I., Ullah A., Khan K.A., Attaullah M., Khan H., Ali H., Bashir M.A., Tahir M., Ansari M.J., Ghramh H.A., Adgaba N., Dash C.K. (2019). Composition and functional properties of propolis (bee glue): A review. Saudi Journal of Biological Sciences, 26(7): 1695-1703.
Bajimaya M.M., Mahotra N.B., Shrestha L., Pradhan S., Malla N., Kandel S., Gurung S. (2021). Manual differential count and automated differential leukocyte count in normal individuals: a comparative study. Journal of Physiological Society of Nepal, 2(1): 21-24.?
Blaxhall P.C., Daisley K.W. (1973). Routine haematological methods for use with fisa blood. Journal of Fish Biology, 5(6):771-781.
Choobkar N. (2017). Persian bee propolis and pollen extracts enhanced the non-specific immune response of rainbow trout (Onchorhynchus mykiss) and resistance against Aeromonas hydrophila. Iranian Journal of Fisheries Science, 16(2): 654-667.
Choobkar N. (2017). Persian bee propolis and pollen extracts enhanced the non-specific immune response of rainbow trout (Onchorhynchus mykiss) and resistance against Aeromonas hydrophila. Iranian Journal of Fisheries Science, 16(2): 654-667.
Dinarello C.A. (2009). Immunological and inflammatory functions of the interleukin-1 family. Annual review of immunology, 27: 519-550.?
Eraslan G., Kanbur M., Silici S. (2007). Evaluation of propolis effects on some biochemical parameters in rats treated with sodium fluoride. Pesticide Biochemistry and Physiology, 88(3): 273-283.
Farag M.R., Abdelnour S.A., Patra A.K., Dhama K., Dawood M.A., Elnesr S.S., Alagawany M. (2021). Propolis: Properties and composition, health benefits and applications in fish nutrition. Fish and Shellfish Immunology, 115: 179-188.
Guo C., Duan Y., Ye W., Zhang W., Cheng Y., Shi M., Xia X.Q. (2023). FishGET: A fish gene expression and transcriptome database with improved accuracy and visualization. Iscience, 26(4): 1-12.
Jung C.H., Jung H., Shin Y.C., Park J.H., Jun C.Y., Kim H.M., Ko S.G. (2007). Eleutherococcus senticosus extract attenuates LPS-induced iNOS expression through the inhibition of Akt and JNK pathways in murine macrophage. Journal of Ethnopharmacology, 113(1): 183-187.?
Khalifa M.A. (2023). Efficacy of dietary propolis supplementation on growth, hematological and immunological responses in Cyprinus carpio L. challenged with Aeromonas hydrophila. University of Baghdad. College of Veterinary Medicine. 173 p.
Lovén J., Orlando D.A., Sigova A.A., Lin C.Y., Rahl P.B., Burge C.B., Young R.A. (2012). Revisiting global gene expression analysis. Cell, 151(3): 476-482.
Martins L.M., Marquez R.F., Yano T. (2002). Incidence of toxic Aeromonas isolated from food and human infection. FEMS Immunology and Medical Microbiology, 32(3): 237-242.
Orsatti C.L., Missima F., Pagliarone A.C., Sforcin J.M. (2010b). Th1/Th2 cytokines’ expression and production by propolis-treated mice. Journal of Ethnopharmacology, 129(3): 314-318.?
Orun I., Erdogan K. (2014). Role of propolis on biochemical and hematological parameters of Oncorhynchus mykiss exposed to cypermethrin. Journal of Survey in Fisheries Sciences, 1(1): 21-35.?
Pagliarone A.C., Missima F., Orsatti C.L., Bachiega T.F., Sforcin J.M. (2009a). Propolis effect on Th1/Th2 cytokines production by acutely stressed mice. Journal of Ethnopharmacology, 125(2): 230-233.?
Parker K., Schimmer B. (2001). Genetics of the development and function of the adrenal cortex. Reviews in Endocrine and Metabolic Disorders, 2(3): 245-252.
Reboucas-Silva J., Celes F.S., Lima J.B., Barud H.S., de Oliveira C.I., Berretta A.A., Borges V.M. (2017). Parasite killing of Leishmania (V) braziliensis by standardized propolis extracts. Evidence-based Complementary and Alternative Medicine, 17(2): 1-14.?
Reis M.I., do Vale A., Pereira P.J., Azevedo J.E., Dos Santos N.M. (2012). Caspase-1 and IL-1? processing in a teleost fish. PLoS One, 7(11): e50450.
Subramani P.A., Narasimha R.V., Balasubramanian R., Narala V.R., Ganesh M.R., Michael R.D. (2016). Cytotoxic effects of Aeromonas hydrophila culture supernatant on peripheral blood leukocytes of Nile tilapia (Oreochromis niloticus): Possible presence of a secreted cytotoxic lectin. Fish and Shellfish Immunology, 58: 604-611.?
Suhail R.N., Mustafa S.A., Al-Obodi E. (2022). Efficiency of silver nanoparticales as antibacterial against Aeromonas hydrophila isolated from infected common carp. Iraqi Journal of Agricultural Sciences, 53(3): 589-597.?
Szliszka E., Kucharska A.Z., Sokó?-??towska A., Mertas A., Czuba Z. P., Król W. (2013). Chemical composition and anti-inflammatory effect of ethanolic extract of Brazilian green propolis on activated J774A. 1 macrophages. Evidence-Based Complementary and Alternative Medicine, 2013: 976415.
Tani H., Hikami S., Takahashi S., Kimura Y., Matsuura N., Nakamura T., Koshino H. (2019). Isolation, identification, and synthesis of a new prenylated cinnamic acid derivative from Brazilian green propolis and simultaneous quantification of bioactive components by LC-MS/MS. Journal of Agricultural and Food Chemistry, 67(44): 12303-12312.?
Touzani S., Embaslat W., Imtara H., Kmail A., Kadan S., Zaid H. Saad B. (2019). In vitro evaluation of the potential use of propolis as a multitarget therapeutic product: physicochemical properties, chemical composition, and immunomodulatory, antibacterial, and anticancer properties. Biomed Research International, 2019(4836378): 11.?
Witeska M., Kondera E., ?ugowska K., Bojarski B. (2022). Hematological methods in fish–Not only for beginners. Aquaculture, 547: 737498.?
Xool-Tamayo J., Chan-Zapata I., Arana-Argaez V.E., Villa-de la Torre F., Torres-Romero J.C., Araujo-Leon J.A., Cauich-Rodríguez J.V. (2020). In vitro and in vivo anti-inflammatory properties of Mayan propolis. European Journal of Inflammation, 18: 1-11.?
Zhang X., Wang G., Gurley E.C., Zhou H. (2014). Flavonoid apigenin inhibits lipopolysaccharide-induced inflammatory response through multiple mechanisms in macrophages. PloS One, 9(9): e107072.
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