Effect of coconut (Cocos nucifera) water, and aqueous extract of Mung bean (Vigna radiata) sprouts and Moringa (Moringa oleifera) leaf on the growth and nutrition of Caulerpa racemosa
Downloads
Caulerpa racemosa, or sea grape, has a high nutritional content and offers many health benefits to humans. The problem faced in cultivating sea grapes is growth, which affects production. This study aimed to determine the effect of different times of soaking C. racemosa in (Cocos nucifera) water, and aqueous extract of Mung bean (Vigna radiata) sprouts and Moringa (Moringa oleifera) leaf on its morphology and growth performance. Fifty grams of C. racemosa was soaked in coconut water and an aqueous extract of moringa leaves and mung bean sprouts at different times and was cultivated for 30 days. This study used a complete randomized design (CRD) with four treatments and three replicates: A (without soaking), B (extracts of Mung bean sprouts and Moringa leaves soaked for 2 hours), C (crude extracts of Mung bean sprouts and Moringa leaves soaked for 4 hours), D (coconut water soaked for 40 minutes), and E (coconut water soaked for 50 minutes). The results showed that the soaking process of C. racemosa in coconut water and aqueous extract of moringa and mung bean sprouts significantly affected the growth and nutrition of C. racemosa. Caulerpa racemosa soaked in coconut water for 40 minutes showed the highest absolute growth (206.66±7.96 g), specific growth (5.45±0.10 %/day), protein (9.11±0.29%), and fiber (1.02±0.20). The morphology of C. racemosa showed that the ramulli, rhizoid, fronds, and stolon were fresh, healthy, and clean. Soaking sea grape seeds in natural PGRs can increase the growth, production, and nutritional content. Further research can be conducted, such as identifying the bioactive substance and its amount in coconut water.
Downloads
Aisa A.T., Suradi., Patahiruddin. (2020). Analysis of the growth rate of seaweed (Gracillaria sp.) immersed in coconut water (Cocos nucifera). Fisheries of Wallacea Journal, 1(1): 31-36.
Anggadiredja J.T., Zatnika A., Purwoto H., Istini S. (2008). Seaweed. Jakarta: Penebar Swadaya. 147 p.
AOAC. (2005). Official methods of analysis (18th ed.). Association of Official Analytical Chemist Inc, USA.
Ariyanti M.Y., Maxiselly., Soleh M.A. (2020). The effect of application of coconut water as a natural growth regulator on the growth of cinchona (Cinchona ledgeriana Moens) after stem formation in marginal areas. Jurnal Agrosintesa, 3(1): 12-23.
Ayyubi N.N.A.A., Kusmanadhi B., Siswoyo T.A., Wijayanto Y. (2019) Effect of concentration of onion extract and coconut water on the growth of green deli honey water guava shoot cuttings (Syzygium samarangense). Berkala Ilmiah Pertanian, 2(1): 19-25.
Brault M., Maldiney R. (1999). Mechanisms of cytokinin action. Plant Physiology and Biochemistry, 37(5): 403-412.
Chen X., Sun Y., Liu H., Liu S., Qin Y., Li P. (2019). Advances in cultivation, wastewater treatment application, bioactive components of Caulerpa lentillifera and their biotechnological applications. PeerJ, (1): 1-15.
Costa M.L., Civello P.M., Chaves A.R., Martinez G.A. (2005) Effect of ethephon and 6-benzylaminopuryne on chlorophyll degrading enzymes and peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20oC. Postharvest Biology and Technology, 35: 191-199.
Culver M., Fanuel T., Chiteka A.Z. (2012). Effect of moringa extract on growth and yield of tomato. Greener Journal of Agricultural Sciences, 2(5): 207-211.
Dascaliuc. (2002). Hormones and synthetic plant growth regulators in agriculture. Institute of Genetics and Plant Physiology, Academy of Sciences of Moldova, 20 Padurii str., Chisinau, Moldova.
Davies P.J. (2010). The plant hormones: Their nature, occurrence, and functions. In: Davies, P.J. (eds) Plant Hormones. Springer, Dordrecht. https://doi.org/ 10.1007/978-1-4020-2686-7_1
Dodds J.H., Roberts L.R. (1982). Experiments in plants tissue culture. Cambridge University Press. Cambridge. 255 p.
Effendi I. (1997). Fisheries biology. Yogyakarta: Yayasan Pustaka Nusantara. 163 p.
Estevez R.E. (2021). Why coconut water is both a biostimulant and an anti-cancer agent. First cycle, G2E. Alnarp: SLU, Department of Biosystems and Technology. Swedish University of Agricultural Sciences.
Fatimah S.N. (2008). Effectiveness of Coconut and Lemon Water on the Growth of Bromeliad Ornamental Plants (Neoregelia carolinae) on Different Media. Universitas Muhammadiyah Surakarta.
Fithriani D. (2015). Opportunities and challenges for developing Caulerpa racemosa as functional foods. KNE Life Sciences, (1): 85-96.
Fu J., Huang B., Zhang G. (2000). Physiological and biochemical changes during seed filling in relation to leaf senescence in soybean. Biologia Plantarum, 43:(4): 545-548
Gaba V.B. (2005). Plant growth regulators in plant tissue culture and development. In: Trigiano and Gray. Plant Development and Biotechnology. London: CRC Press. 348 p.
Gabrielsen B.O. (1996). Historic review on immunological effects of seaweed. The Algae Symposium, Taipei, Taiwan. pp: 10-13.
George E.F. (1993). Plant propagation by tissue culture, Part 1, the Technology. 2nd Edition, Exegetics Limited, Edington. 501 p.
Hermawan D. (2015). Effect of different strains of Kappaphycus alvarezii seaweed on specific growth rates. Jurnal Perikanan dan Kelautan, 5(1): 71-78.
Heyl A., Riefler M., Romanov G., Schmulling T. (2012) Properties, functions and evolution of cytokinin receptors. European Journal of Cell Biology, 91: 246-256.
Ilustrisimo C.A., Palmitos I.C., Senagan R.D. (2013). Growth performance of Caulerpa lentillifera (Lato) in lowered seawater pH. Science and Technology in Partial Fulfillment of the Requirement, Philipinnes, 33 p.
Kandhasamy M., Arunachalam K.D. (2008). Evaluation of in vitro antibacterial property of seaweeds of southeast coast of India. African Journal of Biotechnology, 7(12): 1958-1961.
Khan N., Bano A., Babar M.D.A. (2020). Impacts of plant growth promoters and plant growth regulators on rainfed agriculture. PLoS One, 15(5): e0232926.
Kumar M., Gupta V., Kumari P., Reddy C.R K., Jha B. (2011). Assessment of nutrient composition and antioxidant potential of Caulerpaceae seaweeds. Journal of Food Composition and Analysis, 24(2): 270-278.
Ma’ruf W.F., Ibrahim R., Dewi E.N., Susanto E., Amalia U. (2013). Profile of marine algae Caulerpa racemosa and Gracilaria verrucosa as edible food. Jurnal Saintek Perikanan, 9(1): 68-74.
Mamaril J.C., Paner E.T., Trinidad L.C., Palacpac E.S., Cruz A.D. (1988). Enhancement of seedling growth with extracts from coconut water. Philippine Journal of Crop Science, 13(1): 1-7.
Marliah A., Nurhayati N., Mutia H. (2010). Effect of nasa organic fertilizer and atonik growth regulator on peanut growth and yield (Arachis hypogaea L.). Jurnal Agrista, 14(3): 94-99.
Mintah L.O., Arhin L., Ofusu-Anim J., Nkasah G.O. (2018). Effect of coconut (Cocos nucifera L.) water of different fruit maturity stages on axillary bud initiation, growth and development of plantain (Musa AAB.). Journal of Applied Horticulture, 20(1): 42-47.
Miransari M., Smith D.L. (2014) Plant hormones and seed germination. Environmental and Experimental Botany, 99: 110-121.
Mona A. (2013). The potential of Moringa oleifera extract as a biostimulant in enhancing the growth, biochemical and hormonal contents in rocket (Eruca vesicaria) plants. International Journal of Plant Physiology and Biochemistry, 5: 42-49.
Moubayidin L., Di Mambro R., Sabatini S. (2009). Cytokinin-auxin crosstalk. Trends in Plant Science, 14(10): 557-562.
Nagappan T., Vairappan C.S. (2014). Nutritional and bioactive properties of three edible species of green algae, genus Caulerpa (Caulerpaceae). Journal of Applied Phycology, 26: 1019-1027.
Nana J., Rimmer M., Raharjo S. (2012). Cultivation of Lawi-Lawi (Caulerpa sp.) in ponds as an effort to diversify fisheries cultivation. Jurnal Riset Akuakultur, 2-20.
Owusu S.E. (2020). Growth response of Ixora coccinea (L.) cuttings to coconut water treatment under a low polythene sheet dome. Journal of Agriculture and Ecology, 21(5): 1-8.
Patil M., Desai N., Naraynkar C., Waghmode., Gaikwad D.K. (2022). Effects of plant growth regulators on the carbohydrate accumulation in Simarouba glauca seedlings. Journal of Stress Physiology and Biochemistry, 19(2): 34-39.
Poonsapaya P.M.W., Nabors W., Kersi M., Vajrabhaya. (l989). A comparison of methods for callus culture and plant regeneration of RD-25 rice (Oryza sativa L.) in vitro laboratories. Plant Cell Tissue Organ Culture, 16: 175-186.
Rosniawaty S., Anjasari I.R.D., Sudirja R. (2018) Cytokinin applications for increase plant growth lowland tea. Journal of Industrial and Beverage Crops, 5(1): 31-38
Rosniawaty S., Suherman C., Sudirja R., Istiqomah D.N.A. (2020). Application of several concentrations of coconut water to increase the growth of ICCRI 08 H cocoa cultivar seedlings. Jurnal Kultivasi, 19(2).
Satyavathi V.V., Jauhar P.P., Elias E.M., Rao M.B. (2004). Genomics, molecular genetics and biotechnology effects of growth regulators on in vitro plant regeneration. Crop Science, 44: 1839-1846.
Septiyaningrum I., Utami M.A.F., Johan Y. (2020). Identification of Sea Grape Types (Caulerpa sp.) Sepang Bay, Bengkulu City. Jurnal Perikanan, 10(2): 195-204.
Shah S.H., Islam S., Parrey Z.A., Mohammad F. (2021). Role of exogenously applied plant growth regulators in growth and development of edible oilseed crops under variable environmental conditions: A review. Journal of Soil Science and Plant Nutrition, 21: 3284-3308.
Shah S.H., Parrey Z.A., Islam S., Tyagi A., Ahmad A., Mohammad F. (2022). Exogenously applied sulphur improves growth, photosynthetic efficiency, enzymatic activities, mineral nutrient contents, yield and quality of Brassica juncea L. Sustainability, 14 (14441).
Shah S.H., Islam S., Alamri S., Parrey Z.A., Mohammad F., Kalaji H.M. (2023). Plant growth regulators mediated changes in the growth, photosynthesis, nutrient acquisition and productivity of mustard. Agriculture, 13(570).
Simanjuntak P.T.H., Arifin Z., Mawardi M.K. (2017). The Influence of Production, International Prices and Rupiah Exchange Rates on Indonesian Seaweed Export Volume (Study in 2009–2014). Jurnal Administrasi Bisnis, 50: 163-171.
Sukmadi R.B. (2013). Indole-3-Acetic Acid (IAA) Phytohormone Activity from Several Rhizosphere and Endophyte Bacterial Isolates. Jurnal Sains dan Teknologi Indonesia, 14(3): 221-227.
Wantasen A.S.J., Tamrin. (2012). Feasibility analysis of seaweed cultivation locations in the waters of Dodinga Bay, West Halmahera Regency. Jurnal Perikanan dan Kelautan Tropis, 8(1): 23-27.
Wahyono N.D., Hasanah N., Surachman S.N.P. (2021). The concentration of soaking coconut water and the use of kinds of clon entries toward the growth of bud shoots of Cocoa (Theobroma cacao L). Proceedings of The Third International on Food and Agriculture, 19-27.
Warohmah M., Karyanto A. (2018). The effect of giving two types of natural growth substances on the growth of mangosteen seedlings (Garcinia mangostana L.). Jurnal Agrotek Tropika, 6(1): 15-20.
Widyastuti N., Tjokrokusumo D. (2007). The role of several plant growth regulatory substances in in vitro culture. Jurnal Sains dan Teknologi Indonesia, 3(5): 55-63.
Winata L. (1987). Tissue Culture Techniques. Bogor: PA. 252 p.
Windarto S., Prastiwahyudi A.H., Susilowati T., Haditomo A.H.C., Harwanto D. (2021). Effect of different substrates on growth and protein content of Caulerpa racemosa. Journal of Hunan University Natural Sciences, 48(7).
Xu C.S., Jiang Z., Shen W., Zou S.H. (2018). Toxicological characteristics of plant growth regulators and their impact on male reproductive health. Zhonghua Nan Ke Xue, 24(4): 370-375.
Copyright (c) 2023 International Journal of Aquatic Biology
This work is licensed under a Creative Commons Attribution 4.0 International License.