Effect of Tetraselmis suecica microalgae isolated from the Persian Gulf on the expression level of AKT/mTOR genes in the liver cancer cell line, Huh7

Mahnaz Roshan Cheragh; Hadideh Mabudi

doi:10.22034/ijab.v11i6.1986

Authors

Mahnaz Roshan Cheragh Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
Hadideh Mabudi
[email protected]
Department of Genetics, Khuzestan Science and Research Branch, Islamic Azad University, Ahvaz, Iran.

Vol. 11 No. 6 (2023): December

Articles

December 25, 2023

Downloads

PDF

Abstract
How to Cite
Metrics
References
License

The medicinal and therapeutic properties of microalgae, especially their anti-cancer properties in modulating cellular mechanisms such as cytotoxicity, reducing tumor cell invasion, and increasing apoptosis, have drawn attention to their therapeutic application in cancer. However, there are few reports on the effects of Tetraselmis suecica microalgae on liver cancer. As the aim of this study, after ethanolic extracting from T. suecica, and IC50 determination, the effect of T. suecica microalgae extract on cytotoxicity and apoptosis of Huh7 cells was investigated by MTT and Annexin V/PI staining, respectively. In addition, the expression of AKT/mTOR genes was measured by real-time PCR test. Based on the results, ethanolic extract of T. suecica to the Huh7 cell line significantly decreased the expression of AKT and mTOR genes compared to the control group. The vitality of cancer cells in concentrations of 500 and 1000 µg/ml of T. suecica extract in a 48-hour culture, decreased significantly. In addition, in the 72-hour treatment, a significant decrease in cell viability was observed in the concentrations of 250, 500, and 1000 ?g/ml. According to the results, T. suecica microalgae can offer good benefits as valuable natural materials for the pharmaceutical industry, especially anticancer drugs.

Roshan Cheragh, M., & Mabudi, H. (2023). Effect of Tetraselmis suecica microalgae isolated from the Persian Gulf on the expression level of AKT/mTOR genes in the liver cancer cell line, Huh7. International Journal of Aquatic Biology, 11(6), 563–570. https://doi.org/10.22034/ijab.v11i6.1986

Download Citation

Abd El-Hack M.E., Abdelnour S., Alagawany M., Abdo M., Sakr M.A., Khafaga A.F., Mahgoub S.A., Elnesr S.S., Gebriel M.G. (2019). Microalgae in modern cancer therapy: Current knowledge. Biomedicine and Pharmacotherapy, 111: 42-50.

Bray F., Ferlay J., Soerjomataram I., Siegel R.L., Torre L.A., Jemal A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 68(6): 394-424.

Cha K.H., Koo S.Y., Lee D.U. (2008). Antiproliferative effects of carotenoids extracted from Chlorella ellipsoidea and Chlorella vulgaris on human colon cancer cells. Journal of Agricultural and Food Chemistry, 56(22): 10521-10526.

Chen C., Wang G. (2015). Mechanisms of hepatocellular carcinoma and challenges and opportunities for molecular targeted therapy. World Journal of Hepatology, 7(15): 1964.

Chuang S.C., La Vecchia C., Boffetta P. (2009). Liver cancer: descriptive epidemiology and risk factors other than HBV and HCV infection. Cancer Letters, 286(1): 9-14.

Farooqi A.A., Butt G., Razzaq Z. (2012). Algae extracts and methyl jasmonate anti-cancer activities in prostate cancer: choreographers of ‘the dance macabre’. Cancer Cell International, 12(1): 1-6.

Galicia-Moreno M., Silva-Gomez J.A., Lucano-Landeros S., Santos A., Monroy-Ramirez H.C., Armendariz-Borunda J. (2021). Liver cancer: therapeutic challenges and the importance of experimental models. Canadian Journal of Gastroenterology and Hepatology, 8837811.

Gardeva E., Toshkova R., Yossifova L., Minkova K., Gigova L. (2012). Cytotoxic and apoptogenic potential of red microalgal polysaccharides. Biotechnology and Biotechnological Equipment, 26(4): 3167-3172.

Gloria N.F., Soares N., Brand C., Oliveira F.L., Borojevic R., Teodoro A.J. (2014). Lycopene and beta-carotene induce cell-cycle arrest and apoptosis in human breast cancer cell lines. Anticancer Research, 34(3): 1377-1386.

Holst B., Williamson G. (2008). Nutrients and phytochemicals: from bioavailability to bioefficacy beyond antioxidants. Current Opinion in Biotechnology, 19(2): 73-82.

Huang J., Lok V., Ngai C.H., Chu C., Patel H.K., Thoguluva Chandraseka V., Zhang L., Chen P., Wang S., Lao X.Q., Tse L.A. (2021). Disease burden, risk factors, and recent trends of liver cancer: a global country-level analysis. Liver Cancer, 10(4): 330-345.

Jafari M., Ghadami E., Dadkhah T., Akhavan?Niaki H. (2019). PI3k/AKT signaling pathway: erythropoiesis and beyond. Journal of Cellular Physiology, 234(3): 2373-2385.

Kumar S.R., Hosokawa M., Miyashita K. (2013). Fucoxanthin: A marine carotenoid exerting anti-cancer effects by affecting multiple mechanisms. Marine Drugs, 11(12): 5130-5147.

Lee J.C., Hou M.F., Huang H.W., Chang F.R., Yeh C.C., Tang J.Y., Chang H.W. (2013). Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties. Cancer Cell International, 13: 1-7.

Li Y.J., Li X.F., Yang E.H., Shi M. (2019). Reaserch advances on the role of PI3K/AKT signaling pathway and MiRNA in acute T-Cell lymphocytic leukemia--Review. Zhongguo shi yan xue ye xue za zhi, 27(4): 1344-1347.

Liu C.Y., Chen K.F., Chen P.J. (2015). Treatment of liver cancer. Cold Spring Harbor Perspectives in Medicine, 5(9): a021535.

Nicholson K.M., Anderson N.G. (2002). The protein kinase B/Akt signalling pathway in human malignancy. Cellular Signalling, 14(5): 381-395.

Nitulescu G.M., Van De Venter M., Nitulescu G., Ungurianu A., Juzenas P., Peng Q., Olaru O.T., Gr?dinaru D., Tsatsakis A., Tsoukalas D., Spandidos D.A., Margina D. (2018). The Akt pathway in oncology therapy and beyond. International Journal of Oncology, 53(6): 2319-31.

Prabakaran G., Moovendhan M., Arumugam A., Matharasi A., Dineshkumar R., Sampathkumar P. (2018). Quantitative analysis of phytochemical profile in marine microalgae Chlorella vulgaris. International Journal of Pharmacy and Biological Sciences, 8(2): 562-5.

Parra-Riofrío G., García-Márquez J., Casas-Arrojo V., Uribe-Tapia E., Abdala-Díaz R.T. (2020). Antioxidant and cytotoxic effects on tumor cells of exopolysaccharides from Tetraselmis suecica (Kylin) butcher grown under autotrophic and heterotrophic conditions. Marine Drugs, 18(11): 534.

Sansone C., Galasso C., Orefice I., Nuzzo G., Luongo E,, Cutignano A., Romano G., Christophe Brunet C., Fontana A., Esposito F., Ianora A. (2017). The green microalga Tetraselmis suecica reduces oxidative stress and induces repairing mechanisms in human cells. Scientific Reports, 7(1): 1-12.

Sharawy Z.Z., Ashour M., Abbas E., Ashry O., Helal M., Nazmi H., Kelany M., Kamel A., Hassaan M., Rossi Jr, W., El?Haroun E. (2020). Effects of dietary marine microalgae, Tetraselmis suecica, on production, gene expression, protein markers and bacterial count of Pacific white shrimp Litopenaeus vannamei. Aquaculture Research, 51(6): 2216-2228.