Molecular characterization of gonadotropin-releasing hormone (GnRH) in river catfish, Hemibagrus nemurus (Valenciennes 1840)

Fatin Nabilah Sahadan; Amirah Syafiqah Zamri; Annie Christianus; Muhammad Fadhil Syukri Ismail; Md Yasin Ina-Salwany; Roshani Othman; Zarirah  Zulperi

doi:10.22034/ijab.v11i5.1980

Authors

Fatin Nabilah Sahadan Aquatic Animal Health and Therapeutics Laboratory, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Amirah Syafiqah Zamri Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. https://orcid.org/0000-0002-0525-315X
Annie Christianus Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Muhammad Fadhil Syukri Ismail Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Md Yasin Ina-Salwany Aquatic Animal Health and Therapeutics Laboratory, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia
Roshani Othman Department of Science and Biotechnology, Faculty of Engineering and Life Sciences, Universiti Selangor, Bestari Jaya Campus, Jalan Timur Tambahan, 45600 Batang Berjuntai, Selangor, Malaysia.
Zarirah Zulperi
[email protected]
Aquatic Animal Health and Therapeutics Laboratory, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

Vol. 11 No. 5 (2023): October

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October 25, 2023

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Gonadotropin-releasing hormone (GnRH) is the foremost neuroendocrine peptide required in the reproduction system. Characterization and the involvement of GnRH in fish reproduction, especially in fish species has been complicated by the discovery of multiple GnRH forms. In this paper, we determined the molecular characterization and phylogenetic analysis of GnRH1 and GnRH2 genes in a commercially cultured catfish, Hemibagrus nemurus. This species is a high-demand freshwater fish worldwide especially in the Asia Pacific regions due to its thick flesh and high nutritional value. Problems in their breeding restrict the production of this species in captivity. Therefore, a thorough study of the GnRH genes is important due to their critical role in stimulating the secretion of gonadotropins hormone, which leads to the release of steroid hormones and activates the reproduction system. A complete open reading frame (ORF) of GnRH1 and GnRH2 genes was obtained through PCR amplification and cloned into TOPO® TA Cloning® kit, following sequence assembly and phylogenetic analysis. Phylogenetic analysis revealed the GnRH1 and GnRH2 of H. nemurus were clustered with Siluriformes, consisting of mostly catfish species including Pangasius nasutus and Pangasianodon hypopthalmus. The cDNA of GnRH1 was 371 bp with an ORF of 262 bp encoding a highly variable 81 amino acids, while the cDNA of GnRH2 was 376 bp with an ORF of 260 bp encoding a highly conserved 87 amino acids. This study could offer an advanced idea to develop a new GnRH agonist for artificial breeding of H. nemurus and other catfish sp.

Sahadan, F. N., Zamri , A. S., Christianus, A., Syukri Ismail, M. F., Ina-Salwany, M. Y., Othman, R., & Zulperi, Z. (2023). Molecular characterization of gonadotropin-releasing hormone (GnRH) in river catfish, Hemibagrus nemurus (Valenciennes 1840). International Journal of Aquatic Biology, 11(5), 391–405. https://doi.org/10.22034/ijab.v11i5.1980

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