Biochemical and molecular identification of the isolated bacteria from the beach soil and saline water of Sawa Lake, Iraq
Downloads
This work was conducted to study the biochemical characteristics of the bacterial species isolated from the beach soil, and water of Sawa Lake using molecular and morphological markers. Samples were collected from the water, and surrounding beach area. The results showed the presence of isolates, including Proteus mirabilis, Providencia vermicola, Alcaligenes aquatilis, Raoultella planticola, Enterobacter cloacae, Klebsiella pneumonia, and P. rettgeri. The molecular diagnosis confirmed those of Biochemical traditional markers using 16S gene primers.
Downloads
Abu Samour H., Khatib H. (1999). Geography of Water Resources, Dar Al-Safa Publishing and Distribution, Amman, Hashemite Kingdom of Jordan.
Anzai Y., Kim H., Park J.Y., Wakabayashi H., Oyaizu H. (2000). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. International Journal of Systematic and Evolutionary Microbiology, 50(2): 1563-1589.
Arahal D.R., Ventosa, A. (2002). Moderately halophilic and halotolerant species of Bacillus and related genera. In Appbacteriaions and Systematics of Bacillus and Relatives, pp. 83–99.
Baron E.J., Finegold S.M. (1990). Diagnostic microbiology. 8th ed. The C.V.Mosby Company.
Collee J.G., Miles R.S., Watt B. (1996). Tests for the identification of bacteria. In: Mackie and McCartney practical medical Microbiology by Colle, J.G.; Fraser,A.G.; Marmion,B.P. and Simmons,A. 14th ed, Churchill Livingstone, Singapore. p:131-149.
Galinski E.A. (1993). Compatible solutes of halophilic eubacteria: molecular principles, water-solute interactions, stress protection. Experientia, 49: 487-496.
Jamil A.K. (1977). Geological and hydrogeochemical aspects of Sawa Lake, S. Iraq. ull.Coll.
Kumar S. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Oxford Journal, 35(6): 1547-1549.
Naik M.M., Dubey S.K. (2011). Lead-enhanced siderophore production and alteration in cell morphology in a Pb-resistant Pseudomonas aeruginosa strain 4EA. Current Microbiology, 62(2): 409-414.
Sambrook J., Fritgah E., Maniatis T. (1989). Molecular cloning, a laboratory manual, cold spring Harbour. Laboratory, 145(3): 1365-1373.
Saravanan T., Bhaskaran R., Muthusam M. (2004). Pseudomonas fluorescens induced enzymological changes in Banana roots (cv. Rasthali) against Fusarium with disease. The Plant Pathology Journal, 3: 72-80.
Ventosa A., Nieto J.J., Oren A. (1998). Biology of moderately halophilic aerobic bacteria. Microbiology and Molecular Biology Reviews, 62: 504-544.
wisniewski-Dyé F., Vial L. (2008). Phase and antigenic variation mediated by genome modifications. Antonie Van Leeuwenhoek, 94(4): 493-515.
Zakaria L., Kulaveraasingham H., Guan T.S., Abdullah F., Wan H.Y. (2005). Random amplified polymorphic DNA (RAPD) and random amplified microsatellite (RAMS) of G node infected oil palm and coconut stump in Malaysia. Asia Pacific Journal of Molecular Biology and Biotechnology, 13(1): 236 34.
Islam E., Sar P. (2011). Culture-dependent and -independent molecular analysis of bacterial community within uranium ore. Journal of Basic Microbiology, 4: 1-13.
Copyright (c) 2022 International Journal of Aquatic Biology
This work is licensed under a Creative Commons Attribution 4.0 International License.