The study of enrichment capability of adult Artemia franciscana with singular or combined administration of Pediococcus acidilactici and fructooligosaccharide

Mahmood Azimirad, Said Meshkini, Nasrollah Ahmadifard, Seyed hossein Hoseinifar


The present study investigates the possibility of enriching adult Artemia franciscana with singular or combined administration of Pediococcus acidilactici and fructooligosaccharide (FOS). The experiment was conducted in a completely randomized design with four treatments, including synbiotic, P. acidilactici +FOS (T1), probiotic, P. acidilactici (T2), prebiotic, FOS (T3) and control (T4). To evaluate the enrichment of adult Artemia with each treatment, sampling was performed at 2, 4 and 6 hrs post enrichment. The bacterial counts was measured using the microbial culture and expressed as log CFU per g Artemia. A pre-experiment has been designed and probiotic was used in three levels (107, 108 and 109 CFU per litter of suspension) and prebiotic was used in three levels of 1, 2 and 5 g per litter of suspension. Based on pre experiment results, 108 CFU per litter of probiotic and 5 g per litter of prebiotic was selected. The results of this experiment showed that over time, consumed bacteria increased by adult Artemia and there was a significant difference between sampling in terms of ingested bacteria. The highest bacterial count (6.78±0.03 log CFU g-1) was observed 6 hrs after the start of enrichment. Based on microbial culture, the number of bacteria P. acidilactici in T1 and T2 was significantly higher than those in T4 (control) and T3 (prebiotic). There was no significance difference between T2 (probiotic) and T1 (synbiotic). In conclusion, the results of this study showed that adult Artemia in a short time (about 4 hrs) can retain a large amount of probiotic bacteria.


Probiotic, Synbiotic, P. acidilactici, Enrichment, Artemia franciscana.

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Abid A., Davies S.J., Waines P., Emery M., Castex M., Gioacchini G., Carnevali O., Brickerdike R., Romero J., Merrifield D.L. (2013). Dietary symbiotic application modulates Atlantic salmon (Salmo salar) intestinal microbial communities and intestinal immunity. Fish & Shellfish Immunology, 35(6): 1948-1956.

Agh N., Sorgeloos P. (2005). Handbook of protocols and guidelines for culture and enrichment of live food for use in larviculture. Artemia and Aquatic Animals Research Center, Urmia University, Urmia, 60 p.

Campbell R., Adams A., Tatner M.F., Chair M., Sorgeloos P. (1993). Uptake of Vibrio anguillarum vaccine by Artemia salina as a potential oral delivery system to fish fry. Fish and Shellfish Immunology, 3: 451-459.

Daniels C.L., Merrifield D.L., Ringo E., Davies S.J. (2013). Probiotic, prebiotic, synbiotic applications for the improvement of larval European lobster (Homarus gammarus) culture. Aquaculture, 416: 396-406.

Dhont J., Lavens P. (1996). Tank production and use of ongrown Artemia, In: P. Lavens, P. Soregeloos (Eds.). Manual on the production and use of live food for aquaculture. FAO fisheries technical paper, Rome, 361 p.

Flickinger E.A., Van Loo J., Fahey G.C. (2003). Nutritional responses to the presence of inulin and oligofructose in the diets of domesticated animals: a review. Critical Review Food Science Nutrition, 43:19-60.

Fuller R. (1989). Probiotics in man and animals. Journal Applied bacteriology, 66: 365-366.

Gatesoupe F.J., Ronald Ross W., Victor R.P. (2010). Probiotics and other microbial Manipulations in Fish Feeds: Prospective Health Benefits. Bioactive Foods in Promoting Health, Boston, Academic Press, Pp: 541-552.

Gibson G.R. (2004). Fibre and effects on probiotics (the prebiotic concept). Clinical Nutrition Supplements, 1: 25-31.

Gomez-Gil B., Herrera-Vega M.A., Aberu Grobis F. A., Roque A. (1998). Bioencapsulation of two different Vibrio species in nauplii of the Brine shrimp (Artemia fransiscana). Applied Environmental microbiology, 64: 2318-2322.

Havennar R., Ten Brink B., Huisint J.H.J. (1992). Selection of strains for probiotic use. In: R. Fuller (ed.). Probiotics, the scientific basis. Chapman and Hall, London, pp: 209-224.

Hoseinifar S.H., Mirvaghefi A., Amoozegar M.A., Sharifian M., Esteban M.A. (2015). Modulation of innate immune response, mucosal parameters and disease resistance in rainbow trout (Oncorhyncchus mykiss) upon symbiotic feeding. Fish and Shellfish Immunology, 45: 27-32.

Mahious A.S., Ollevier F. (2005). Probiotics and Prebiotics in Aquaculture: A review. In: N. Agh, P. Sorgeloos (eds). 1st regional workshop on techniques for enrichment of live food for use in larviculture. Urmia, Iran, pp: 17-26.

Makridis P., Fjellheim J.A., Skjermo J., Vadstein O. (2000). Colonization of the gut in first feeding turbot by bacterial strains added to the water or bioencapsulated in rotifers. Aquaculture International, 8: 367-380.

Merrifield D.L., Dimitroglou A., Foey A., Davies S.J., Baker R.T.M. (2010). The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture, 302: 1-18.

Montajami S., Hajiahmadyan M., Forouhar Vajargah M., Hosseini Zarandeh A.S., Shirood Mirzaie F., Hosseini S.A. (2012). Effect of symbiotic (Biomin imbo) on growth performance and survival rate of Texas cichlid (Herichthys cyanoguttatus) larvae. Global Vertebrate, 9(3): 358-361.

Olafsen J.A. (2001). Interactions between fish larvae and bacteria in marine aquaculture. Aquaculture, 200: 223-247.

Parta S.K., Mohamed K.S. (2003). Enrichment of Artemia nauplii with the probiotic yeast Sacharomyces boulardii and its resistance against a pathogenic vibrio. Aquaculture international, 11: 505-514.

Peter H., Sneath A. (1986). Bergeys manual of systematic Bacteriology, 1104-1154.

Reitan K.I., Rainuzzo J.R., Oie G., Olsen Y. (1993). Nutritional effects of algal addition in first feeding tanks of turbot Scophthalmus maximus L. larvae. Aquaculture, 118: 257-275.

Rengpipat S., Phianphak W., Piyatiratitivorakul S., Menasveta P. (1998). Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth. Aquaculture, 167: 301-313.

Ringo E., Sinclair P.D., Birkbeck H., Barbour A. (1992). Production of eicosapentaenoic acid 20:5 n-3 by Vibrio pelagius isolated from turbot Scophthalmus maximus L. larvae. Applied Environment Microbiology, 58: 3777-3778.

Ritar A.J., Dunstan G.A., Nelson M.M., Brown M.R., Nichols P.D., Thomas G.W., Smith E.G., Crear B.J., Kolkovski S. (2004). Nutritional and bacterial profiles of juvenile Artemia fed different enrichments and during starvation. Aquaculture, 239: 351-373.

Roberfroid M. (2007). Prebiotics: the concept revisited. The Journal of Nutrition, 137:830s.

Rodriguez-Estrada U., Satoh S., Haga Y., Fushimi H., Sweetman J. (2009). Effects of single and combined supplementation of Enterococcus faecalis, mannanoligosaccharide and polyhydrobutyric acid on growth performance and immune response of rainbow trout Oncorhynchus mykiss. Aquaculture Science, 57: 609-617.

Sorgeloos P., Dhert P., Candreva P. (2001). Use of the brine shrimp, Artemia spp., in marine fish larviculture. Aquaculture, 200:147-159.

Sorgeloos P., Lavens P., Leger P., Tackaert W., Versichele D. (1986). Manual for the culture and use of brine shrimp Artemia in aquaculture. State University of Ghent, Belgium. 319 p.

Teresita D.N.J., Maldonado-Montiel Leticia G. (2005). Biomass production and nutritional value of Artemia spp. (Anostraca: Artemiidae) in Campeche. Mexico Revista de Biological Tropical, 53(3-4): 447-454.

Ziaei-Nejad S., Rezaei M.H., Takami G.A., Lovett D.L., Mirvaghefi A.R., Shakouri M. (2006). The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. Aquaculture, 252: 516-524.


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