Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises

Venipuncture procedure Southern River terrapin Subcarapacial venous plexus Jugular vein Genomic.

Authors

  • Mohd Hairul Mohd Salleh Department of of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Yuzine Esa
    yuzine@upm.edu.my
    Royal Malaysian Customs Department, Persiaran Perdana, Presint 2, 62596 Putrajaya, Malaysia.
June 14, 2022
turtles and tortoises

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This technical note aims to describe the venipuncture procedure used to collect blood from Southern River terrapins via the subcarapacial venous plexus (SVP) and jugular vein. On uncooperative terrapins, SVP was applied while the jugular vein was reversed. 1.5 ml blood was preserved in 0.5 ml EDTA and stored at -20°C. ReliaPrepTM Blood genomic DNA Miniprep was used to extract DNA. Thermo ScientificTM NanoDrop 2000c was used to determine the concentrations of extracted DNAs. The greatest concentration of DNA is 136.3 g/L, and the highest purity is 1.90. The treatment is safe, minimally invasive, and effective.