Assessment of flocculation induced by pH increase for harvesting microalgae Cyanothece sp.

Zahra Aminikhoei; Elnaz Erfani far; Saaedeh Taherpanah; Mahsa Naderi Samani

Authors

Zahra Aminikhoei
[email protected]
Agricultural Research Education and Extension Organization (AREEO), Iranian Fisheries Science Research Institute (IFSRI), offshore fisheries Research Center, Chabahar, Iran.
Elnaz Erfani far Agricultural Research Education and Extension Organization (AREEO), Iranian Fisheries Science Research Institute (IFSRI), offshore fisheries Research Center, Chabahar, Iran.
Saaedeh Taherpanah Iranian Biological Resource Center, Tehran, Iran.
Mahsa Naderi Samani Iran Fisheries Organization, Tehran, Iran.

Vol. 9 No. 5 (2021): October

Articles

December 2, 2021

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One of the most important challenges lies in the microalgae mass production is the high cost of harvesting process which is the separation of a low amount of biomass consisting of small individual cells from a large volume of culture medium. Therefore, finding an efficient and cost-effective technique for harvesting microalgae is important issue. In the current study, pH-induced flocculation method was tested for microalgae Cyanothece sp. harvesting. The halophilic microalgae were cultured and grown in laboratory with hypersaline water in F/2 medium. After reaching the stationary phase, the impact of pH induction (from natural medium culture pH:8.2 to pH:11) on flocculation efficiency, chlorophyll a, chlorophyll b, total carotenoid, Î²-Carotene and phycocyanin component and the possibility of reuse flocculated medium of microalgae Cyanothece sp. were evaluated. The results indicated that the increasing the medium pH value by adding NaOH from pH natural at 8.2 to 9.4 increased flocculation efficiency significantly from 10 up to 90% (P<0.05), but after that remained stable up to pH: 11. Regarding the pigment content, the increase in pH value from natural pH: 8.2 to pH: 9.1 had a relatively a medium effect on pigment components, including chlorophyll a, chlorophyll b, total carotenoid, Î²-Carotene and phycocyanin amount of the harvested biomass of Cyanothece sp, but after that from pH: 9.4 to 11 the reduction was severe. The medium culture from pH: 8.5 to pH: 11 was reusable for new culture of microalgae. Thus, the flocculation induced by pH increase up to pH: 9.1 is a suitable method for harvesting microalgae Cyanothece sp. with no serious adverse effect on pigment component.

Aminikhoei, Z., Erfani far, E., Taherpanah, S., & Naderi Samani, M. (2021). Assessment of flocculation induced by pH increase for harvesting microalgae Cyanothece sp. International Journal of Aquatic Biology, 9(5), 326–332. Retrieved from http://ij-aquaticbiology.com/index.php/ijab/article/view/1393

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Ahmed R.A., He M., Aftab R.A., Zheng S., Nagi M., Bakri R., Wang C. (2017). Bioenergy application of Dunaliella salina SA 134 grown at various salinity levels for lipid production. Scientific Reports, 7: 1-10.

Arnon A. (1967). Method of extraction of chlorophyll in the plants. Agronomy Journal, 23: 112-121.

Ben-Amotz A. (1999). Dunaliella Î²-carotene. In Enigmatic microorganisms and life in extreme environments Springer, Dordrecht. pp: 399-410.

Bandyopadhyay A., Elvitigala T., Welsh E., Stöckel J., Liberton M., Min H., Sherman L.A. Pakrasi H.B. (2011). Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing cyanobacteria. MBio, 2(5): 00214-11.

Borowitzka M.A. (2013a). Dunaliella: biology, production, and markets Handbook of microalgal culture Wiley. pp: 359-368.

Borowitzka M.A. (2013b). High-value products from microalgae"”their development and commercialisation. Journal of Applied Phycology, 25: 743-756.

Borowitzka M.A., Borowitzka L.J. (1988). Micro-algal biotechnology. Cambridge University Press. 477 p.

Branyikova I., Prochazkova G., Potocar T., Jezkova Z., Branyik T. 2018. Harvesting of microalgae by flocculation. Fermentation, 4(4): 93-105.

Cardoso L.C., Serrano C.M., Rodríguez M.R., de la Ossa E.J.M., Lubián L.M. (2012). Extraction of carotenoids and fatty acids from microalgae using supercritical technology. American Journal of Analytical Chemistry, 3: 877-883.

Chi Z., Su C.D., Lu W.D. (2007). A new exopolysaccharide produced by marine Cyanothece sp. 113. Bioresource Technology, 98(6): 1329-1332.

Gerde J.A., Yao L., Lio J., Wen, Z., Wang T. (2014). Microalgae flocculation: impact of flocculant type, algae species and cell concentration. Algal Research, 3: 30-35.

Guillard R.R.L, Ryther J.H. (1962). Studies of marine planktonic diatoms: I. Cyclotella nana Hustedt, and Detonula conferraceae (Cleve) gran. Canadian Journal of Microbiology, 8(2): 229-239.

Hejazi M.A., Kleinegris D., Wijffels R.H. (2004). Mechanism of extraction of Î²"carotene from microalga Dunaliellea salina in two"phase bioreactors. Biotechnology and Bioengineering, 88(5): 593-600.

Horiuchi J-I., Ohba I., Tada K., Kobayashi M., Kanno T., Kishimoto M. (2003). Effective cell harvesting of the halotolerant microalga Dunaliella tertiolecta with pH control. Journal of Bioscience and Bioengineering , 95: 412-415.

Kumar D., Kumar N., Pabbi S. (2013). Protocol optimization for enhanced production of pigments in Spirulina. Indian Journal of Plant Physiology, 18(3): 308-312.

Kwon H., Lu M., Lee E.Y., Lee J. (2014). Harvesting of microalgae using flocculation combined with dissolved air flotation. Biotechnology and Bioprocess Engineering, 19:143-149.

Lichtenthaler H.K. (1987). Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in Enzymology, 148: 350-382.

Liu J et al. (2013) Freshwater microalgae harvested via flocculation induced by pH decrease. Biotechnology for Biofuels, 6(1): 1-11.

Maji G., Choudhury S., Hamid S., Prashanth R., Sibi G. (2018). Microalgae harvesting via flocculation: impact of pH, algae species and biomass concentration. Methods of Microbiology and Molecular Biology, 1(2): 106.

Moheimani N.R. (2013). Long-term outdoor growth and lipid productivity of Tetraselmis suecica, Dunaliella tertiolecta and Chlorella sp. (Chlorophyta) in bag photobioreactors. Journal of Applied Phycology, 25: 167-176.

Pérez L., Salgueiro J.L., Maceiras R., Cancela í., Sánchez í. (2017). An effective method for harvesting of marine microalgae: pH induced flocculation. Biomass and Bioenergy, 97: 20-26.

Sanyano N., Chetpattananondh P., Chongkhong S. (2013). Coagulation–flocculation of marine Chlorella sp. for biodiesel production. Bioresource Technology, 147: 471-476.

Spilling K., Seppälä J., Tamminen T. (2011). Inducing autoflocculation in the diatom Phaeodactylum tricornutum through CO2 regulation. Journal of Applied Phycology, 23: 959-966.

Spolaore P., Joannis-Cassan C., Duran E., Isambert A. (2006). Commercial applications of microalgae. Journal of bioscience and bioengineering, 101: 87-96.

Vandamme D., Beuckels A., Markou G., Foubert I., Muylaert K. (2015). Reversible flocculation of microalgae using magnesium hydroxide BioEnergy Research, 8: 716-725.

Wu Z., Zhu Y., Huang W., Zhang C., Li T., Zhang Y., Li A. (2012). Evaluation of flocculation induced by pH increase for harvesting microalgae and reuse of flocculated medium. Bioresource Technology, 110: 496-502.

Yang F., Xiang W., Fan J., Wu H., Li T., Long L. (2016). High pH-induced flocculation of marine Chlorella sp. for biofuel production. Journal of Applied Phycology, 28: 747-756.

Zhu C.J., Lee, Y.K. (1997). Determination of biomass dry weight of marine microalgae. Journal of applied phycology, 9(2): 189-194.

Zhang X., Yang S., Sun J., Wu C., Wang J., Zhang G., Ding C. (2018). Morphology, ultrastructure and phylogeny of Cyanothece sp. (Cyanobacteriaceae: Cyanophyceae) isolated from the eastern Indian Ocean. Acta Oceanologica Sinica, 37(10): 4-10.