Genetic characterization of Garra rufa (Heckel, 1843) populations in Tigris Basin, Iran using microsatellite markers

Hamed Kolangi Miandare; Ghasem Askari; Ali Shabany; Hamid Reza Rezaei

doi:10.22034/ijab.v4i1.123

Authors

Hamed Kolangi Miandare
[email protected]
Department of Fishery, Gorgan University of Agricultural Sciences and Natural Resources, Iran.
Ghasem Askari Department of Fishery, Gorgan University of Agricultural Sciences and Natural Resources, Iran.
Ali Shabany Department of Fishery, Gorgan University of Agricultural Sciences and Natural Resources, Iran.
Hamid Reza Rezaei Department of Fishery, Gorgan University of Agricultural Sciences and Natural Resources, Iran.

Vol. 4 No. 1 (2016): February

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April 1, 2016

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The isolation-by-distance theory states that the genetic differentiation between individuals raised by increasing geographic distance. Therefore, this study tested this hypothesis for Garra rufa, a freshwater fish species of Iranian inland waters, from six rivers located at the different distances in Tirgis basin. For this purpose, eight variable microsatellite loci were applied to identify geographic-based population structure of G. rufa. From 240 fish of six populations, 102 alleles were found with a mean number of 11.625 to 13.250 alleles. Heterozygosity was ranged 0.567-0.638 in six studied populations. Moreover, a significant deviations from Hardy-Weinberg were found in the studied populations. Unweight pair group analysis indicated that the six studied populations could be divided into four major clusters. The results revealed a fairly high level of genetic variation in the microsatellite loci within six studied populations. Wright's fixation index (Fst) ranged between 0.013-0.044 indicating little genetic differentiation between populations. Within this range, however, we found a strong positive relation between Fst and geographical distance lending support to the isolation-by-distance theory.

Kolangi Miandare, H., Askari, G., Shabany, A., & Rezaei, H. R. (2016). Genetic characterization of Garra rufa (Heckel, 1843) populations in Tigris Basin, Iran using microsatellite markers. International Journal of Aquatic Biology, 4(1), 57–68. https://doi.org/10.22034/ijab.v4i1.123

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