Effect of density on some physiological responses to transportation stress in Mesopotamichthys sharpeyi (Günther 1874) fingerlings

Tayebe Nazari, Vahid Yavari, Amir Parviz Salati, Abdoali Movahedinia

Abstract

In the present study, the effect of density on transportation stress in Mesopotamichthys sharpey fingerlings was evaluated. For this purpose, four different densities, including 40, 80, 120 and 160 g/l were used as treatments each with 3 replicates. Simulation of transport procedure was carried out for 4 hrs. The blood samples were collected from the fish prior to loading from the stocking tank (control), after 4 hrs of transportation and from released fish into recovery glass tanks at 6, 12, 24, 48 and 96 hrs after transportation during recovery period. For blood sampling, fish immediately anesthetized by adding 2% 2-phenoxy ethanol and the blood samples were prepared. The cortisol, glucose and lactate value of plasma were measured. The results showed a significant increase in cortisol and glucose levels (in highest density) after transportation in all treatments (P<0.05). Lactate did not show a significant difference in experimental groups (P>0.05). The results showed that only cortisol level was significantly different with basal level at 96 hrs. Our findings showed that this species can be transported at higher densities up to 120 g/l.

Keywords

Stress, Cortisol, Lactate, Glucose, Mesopotamichthys sharpeyi.

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References

Abreu J., Takahashi L., Hoshiba M., Urbinati E. (2009). Biological indicators of stress in pacu (Piaractus mesopotamicus) after capture. Brazilian Journal of Biology, 69(2): 415-421.

Abreu J.S., Sanabria-Ochoa A.I., Gonçalves F.D., Urbinati E.C. (2008). Stress responses of juvenile matrinxã (Brycon amazonicus) after transport in a closed system under different loading densities. Ciência Rural, 38(5): 1413-1417.

Acerete L., Balasch J., Espinosa E., Josa A., Tort L. (2004). Physiological responses in Eurasian perch (Perca fluviatilis, L.) subjected to stress by transport and handling. Aquaculture, 237(1): 167-178.

Adamante W., Nuñer A., Barcellos L., Soso A., Finco J. (2008). Stress in Salminus brasiliensis fingerlings due to different densities and times of transportation. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 60(3): 755-761.

Ashley P.J. (2007). Fish welfare: current issues in aquaculture. Applied Animal Behaviour Science, 104(3): 199-235.

Barton B.A. (2002). Stress in fishes: a diversity of responses with particular reference to changes in circulating corticosteroids. Integrative and Comparative Biology, 42(3): 517-525.

Bolasina S.N. (2011). Stress response of juvenile flounder (Paralichthys orbignyanus, Valenciennes 1839), to acute and chronic stressors. Aquaculture, 313: 140-143.

Carmichael G., Wedemeyer G., McCraren J., Millard J. (1983). Physiological effects of handling and hauling stress on smallmouth bass. The Progressive Fish-Culturist, 45(2): 110-113.

Carneiro P.C.F., Kaiseler P.H.S., Swarofsky E.A.C., Baldisserotto B. (2009). Transport of jundiá Rhamdia quelen juveniles at different loading densities: water quality and blood parameters. Neotropical Ichthyology, 7(2): 283-288.

Conte F. (2004). Stress and the welfare of cultured fish. Applied Animal Behavior Science, 86(3): 205-223.

Dobšíková R., Svobodová Z., Bláhová J., Modrá H., Velíšek J. (2006). Stress responses to long distance transportation of common carp (Cyprinus carpio L.). Acta veterinaria, 75: 437-448.

Dobšíková R., Svobodová Z., Bláhová J., Modrá H., Velíšek J. (2009). The effect of transport on biochemical and haematological indices of common carp (Cyprinus carpio L.). Czech Journal of Animal Science, 54 (11): 510-518.

Fazio E., Ferlazzo A. (2003). Evaluation of stress during transport. Veterinary Research Communications, 27: 519-524.

Gaedi G., Yavari V., Falahatkar B., Nikbakht G., Sheibani M.T., Salati A.P. (2013). Whole egg and alevin changes of cortisol and interrenal tissue differences in rainbow trout Oncorhynchus mykiss exposed to different stocking densities during early development. Zoological Science, 30(12): 1102-1109.

Gbore F.A., Oginni O., Adewole A.M., Aladeton J.O. (2006). The effect of transportation and handling stress on haematology and plasma biochemistry in fingerling of Clarias gariepinus and Tilapia zillii. World Journal of Agriculture Sciences, 2 (2): 208-212.

Grutter A., Pankhurst N. (2000). The effects of capture, handling, confinement and ectoparasite load on plasma levels of cortisol, glucose and lactate in the coral reef fish Hemigymnus melapterus. Journal of Fish Biology, 57(2): 391-401.

Harmon T.S. (2009). Methods for reducing stressors and maintaining water quality associated with live fish transport in tanks: a review of the basics. Aquaculture, 1(1): 58-66.

Hur J.W., Park I.S., Chang Y.J. (2006). Physiological responses of the olive flounder, Paralichthys olivaceus, to a series stress during the transportation process. Ichthyological Research, 54(1): 32-37.

Iversen M., Finstad B., Nilssen K.J. (1998). Recovery from loading and transport stress in Atlantic salmon (Salmo salar L.) smolts. Aquaculture, 168: 387-394.

Iversen M., Finstad B., McKinley R.S., Eliassen R.A., Carlsen K.T., Evjen T. (2005). Stress responses in Atlantic salmon (Salmo salar L.) smolts during commercial well boat transports, and effects on survival after transfer to sea. Aquaculture, 243(1): 373-382.

Kayali B., Yigit M., Bulut, M. (2011). Evaluation of the Recovery Time of Sea Bass (Dicentrarchus Labrax Linnaeus, 1758) Juveniles from transport and handling stress: using ammonia nitrogen excretion rates as a stress indicator. Journal of Marine Science and Technology, 19(6): 102-106.

Kubilay A., Uluköy G. (2002). The effects of acute stress on rainbow trout (Oncorhynchus mykiss). Turkish Journal of Zoology, 26: 249-254.

Martinez-Alvarez R., Hidalgo M., Domezain A., Morales A., García-Gallego M.A., Sanz A. (2002). Physiological changes of sturgeon Acipenser naccarii caused by increasing environmental salinity. Journal of Experimental Biology, 205(23): 3699-3706.

Nomura M., Sloman K., Von Keyserlingk M., Farrell A. (2009). Physiology and behavior of Atlantic salmon (Salmo salar) smolts during commercial land and sea transport. Physiology and behavior, 96(2): 233-243.

Pavlidis M., Angellotti L., Papandroulakis N., Divanach P. (2003). Evaluation of transportation procedures on water quality and fry performance in red porgy (Pagrus pagrus) fry. Aquaculture, 218(1): 187-202.

Standard Method for examination of water and wastewater. (1992). published by American Public Health Association, Washington DC, USA.

Salati A.P., Baghbanzadeh A., Soltani M., Peyghan R., Riazi G.H. (2010). The responses of plasma glucose, lactate, protein and haematological parameters to osmotic challenge in Common carp (Cyprinus carpio). International and Journal of Veterinary Research, 4: 49-52.

Sulikowski J.A., Fairchild E.A., Rennels N., Howell W.H., Tsang P.C.W. (2006). The effects of transport density on cortisol levels in juvenile winter flounder, Pseudopleuronectes americanus. Journal of the World Aquaculture Society, 37(1): 107-112.

Svobodova Z., Kalab P., Dusek L., Vykusova B. (1999). The effect of handling and transport on the concentration of glucose and cortisol in the blood plasma of common carp. Acta Veterinaria Brno, 68: 265-274.

Urbinati E.C., De Abreu J.S., da Silva Camargo A.C., Landinez Parra M.A. (2004). Loading and transport stress of juvenile matrinxã (Brycon cephalus, Characidae) at various densities. Aquaculture, 229 (1): 389-400.

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