Isolation, characterization and molecular identification of potential probiotic lactobacilli from gastrointestinal tracts of Capoeta razii and Aristichthys nobilis

Meysam Rameshgar; Mohamad Reza Ghomi Marzdashti; Seyed Masoud Hashemi Karoui; Syed Mahdi Hoseini Fard; Seysd Reza Tabari Pour

Authors

Meysam Rameshgar
[email protected]
Department of Aquaculture, Islamic Azad University, Tonekabon branch, Tonekabon, Iran.
Mohamad Reza Ghomi Marzdashti Department of Aquaculture, Islamic Azad University, Tonekabon branch, Tonekabon, Iran.
Seyed Masoud Hashemi Karoui Department of Microbiology, Islamic Azad University, Babol branch, Babol, Iran.
Syed Mahdi Hoseini Fard Department of Aquaculture, Islamic Azad University, Babol branch, Babol, Iran.
Seysd Reza Tabari Pour Department of Cellular and Molecular, Islamic Azad University, Babol branch, Babol, Iran.

Vol. 8 No. 6 (2020): December

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September 25, 2020

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The aim of this study was to isolate and identify Lactobacillus with probiotic potential from gastrointestinal tracts (GIT) of Capoeta razii and bighead carp, Aristichthys nobilis. GIT samples of fifteen bighead carp and sixteen C. razii were collected and homogenized in 0.9% NaCl buffer and cultured on MRS medium. Nine isolates were obtained from C. razii and 18 insolates from bighead carp. The isolates were sequentially subjected to acid- and bile salt-resistance test. Three isolates from C. razii and one isolate from bighead carp were both acid- and bile salt-resistant. These isolates were identified by sequencing 16s rRNA and subjected to BLAST analysis to find the highest similarity in GeneBank. The isolate from bighead carp and two isolates from C. razii were L. plantarum with the highest similarity with strain DR7. One of the isolates from C. razii was L. brevis with the highest similarity with strains 100D8 and BDGP6. Further studies are encouraged to assess these probiotic effects in aquaculture species growth performance and health status.

Rameshgar, M., Ghomi Marzdashti, M. R., Hashemi Karoui, S. M., Hoseini Fard, S. M., & Tabari Pour, S. R. (2020). Isolation, characterization and molecular identification of potential probiotic lactobacilli from gastrointestinal tracts of Capoeta razii and Aristichthys nobilis. International Journal of Aquatic Biology, 8(6), 396–403. Retrieved from http://ij-aquaticbiology.com/index.php/ijab/article/view/1003

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Ahmadifar E., Moghadam M.S., Dawood M.A., Hoseinifar S.H. (2019). Lactobacillus fermentum and/or ferulic acid improved the immune responses, antioxidative defence and resistance against Aeromonas hydrophila in common carp (Cyprinus carpio) fingerlings. Fish and Shellfish Immunology, 94: 916-923.

Alderman D., Hastings T. (1998). Antibiotic use in aquaculture: development of antibiotic resistance–potential for consumer health risks. International Journal of Food Science and Technology, 33: 139-155.

Allameh S.K., Ringí¸ E., Yusoff F.M., Daud H.M., Ideris A. (2014). Properties of Enterococcus faecalis, a new probiotic bacterium isolated from the intestine of snakehead fish (Channa striatus Bloch). African Journal of Microbiology Research, 8: 2215-2222.

Balcázar J.L., Vendrell D., de Blas I., Ruiz-Zarzuela I., Muzquiz J.L., Girones O. (2008). Characterization of probiotic properties of lactic acid bacteria isolated from intestinal microbiota of fish. Aquaculture (Amsterdam, Netherlands), 278: 188-191.

Banerjee S.P., Dora K.C., Chowdhury S. (2013). Detection, partial purification and characterization of bacteriocin produced by Lactobacillus brevis FPTLB3 isolated from freshwater fish. Journal of Food science and Technology, 50: 17-25.

Castex M., Lemaire P., Wabete N., Chim L. (2010). Effect of probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress of Litopenaeus stylirostris under Vibrio nigripulchritudo challenge. Fish and Shellfish Immunology, 28: 622-631.

Castillo S., Rosales M., Pohlenz C., Gatlin III D.M. (2014). Effects of organic acids on growth performance and digestive enzyme activities of juvenile red drum Sciaenops ocellatus. Aquaculture (Amsterdam, Netherlands), 433: 6-12.

Chong H.-X., Yusoff N.A.A., Hor Y.-Y., Lew L.-C., Jaafar M.H., Choi S.-B., Yusoff M.S., Wahid N., Abdullah M.F.I., Zakaria N. (2019a). Lactobacillus plantarum DR7 improved upper respiratory tract infections via enhancing immune and inflammatory parameters: A randomized, double-blind, placebo-controlled study. Journal of Dairy Science, 102: 4783-4797.

Chong H., Yusoff N., Hor Y.-Y., Lew L.-C., Jaafar M., Choi S.-B., Yusoff M., Wahid N., Abdullah M., Zakaria N. (2019b). Lactobacillus plantarum DR7 alleviates stress and anxiety in adults: a randomised, double-blind, placebo-controlled study. Beneficial Microbes, 10: 355-373.

Ebrahimi M., Daeman N.H., Chong C.M., Karami A., Kumar V., Hoseinifar S.H., Romano N. (2017). Comparing the effects of different dietary organic acids on the growth, intestinal short-chain fatty acids, and liver histopathology of red hybrid tilapia (Oreochromis sp.) and potential use of these as preservatives. Fish Physiology and Biochemistry, 43: 1195-1207.

FAO. (2020). FAO statistics. Global aquaculture production. Available at http://www.fao.org/fishery /statistics/global-aquaculture-production/en

FeÄkaninová A., KošÄová J., MudroÅˆová D., Schusterová P., MarušÄáková I.C., Popelka P. (2019). Characterization of two novel lactic acid bacteria isolated from the intestine of rainbow trout (Oncorhynchus mykiss, Walbaum) in Slovakia. Aquaculture (Amsterdam, Netherlands), 506: 294-301.

Ghanbari M., Rezaei M., Jami M., Nazari R. (2009). Isolation and characterization of Lactobacillus species from intestinal contents of beluga (Huso huso) and Persian sturgeon (Acipenser persicus). Iranian Journal of Veterinary Research, 10: 152-157.

Ghelichpour M., Rajabiesterabadi H., Hoseini S.M. (2016). Gill histopathological characteristics of Caspian roach (Rutilus rutilus caspicus) fingerlings treated with potassium permanganate and formalin. Aquaculture Research, 47: 276-282.

Ghosh S., Ringo E., Selvam A.D.G., Rahiman K.M., Sathyan N., John N., Hatha A. (2014). Gut associated lactic acid bacteria isolated from the estuarine fish Mugil cephalus: molecular diversity and antibacterial activities against pathogens. International Journal of Aquaculture, 4.

Giri S.S., Sukumaran V., Oviya M. (2013). Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita. Fish and Shellfish Immunology, 34: 660-666.

He W., Rahimnejad S., Wang L., Song K., Lu K., Zhang C. (2017). Effects of organic acids and essential oils blend on growth, gut microbiota, immune response and disease resistance of Pacific white shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus. Fish and Shellfish Immunology, 70: 164-173.

Hoseini S.M., Tarkhani R. (2013). Serum biochemical characteristics of Carassius auratus (L) following short-term formalin or NaCl treatment. International Journal of Aquatic Biology, 1: 14-21.

Hoseini S.M., Yousefi M. (2019). Beneficial effects of thyme (Thymus vulgaris) extract on oxytetracycline-induced stress response, immunosuppression, oxidative stress and enzymatic changes in rainbow trout (Oncorhynchus mykiss). Aquaculture Nutrition, 25: 298-309.

Hoseini S.M., Mirghaed A.T., Iri Y., Ghelichpour M. (2018). Effects of dietary cineole administration on growth performance, hematological and biochemical parameters of rainbow trout (Oncorhynchus mykiss). Aquaculture (Amsterdam, Netherlands), 495: 766-772.

Hoseini S.M., Yousefi M., Hoseinifar S.H., Van Doan H. (2019). Antioxidant, enzymatic and hematological responses of common carp (Cyprinus carpio) fed with myrcene- or menthol-supplemented diets and exposed to ambient ammonia. Aquaculture (Amsterdam, Netherlands), 506: 246-255.

Hoseini S.M., Mirghaed A.T., Paray B.A., Hoseinifar S.H., Van Doan H. (2020). Effects of dietary menthol on growth performance and antioxidant, immunological and biochemical responses of rainbow trout (Oncorhynchus mykiss). Aquaculture (Amsterdam, Netherlands), 735260.

Hoseinifar S.H., Hoseini S.M., Bagheri D. (2017). Effects of galactooligosaccharide and Pediococcus acidilactici on antioxidant defence and disease resistance of rainbow trout, Oncorhynchus mykiss. Annals of Animal Science, 17: 217-227.

Hoseinifar S.H., Mirvaghefi A., Amoozegar M.A., Sharifian M., Esteban M.í. (2015). Modulation of innate immune response, mucosal parameters and disease resistance in rainbow trout (Oncorhynchus mykiss) upon synbiotic feeding. Fish and Shellfish Immunology, 45: 27-32.

Kavitha M., Raja M., Perumal P. (2018). Evaluation of probiotic potential of Bacillus spp. isolated from the digestive tract of freshwater fish Labeo calbasu (Hamilton, 1822). Aquaculture Reports, 11: 59-69.

Khodadadi M., Abbasi N., Adorian T.J., Farsani H.G., Hedayati A., Hoseini S.M. (2018). Growth performance, survival, body composition, hematological parameters, intestinal histomorphology, and digestive enzymes' activity in juvenile rainbow trout (Oncorhynchus mykiss) fed dietary Immunogen®. Journal of Applied Aquaculture, 30: 174-186.

Lee C.-S., Lim C., Webster C.D., (2015). Dietary nutrients, additives, and fish health. Wiley-Blackwell, NJ, USA. 355 p.

Liu W., Ren P., He S., Xu L., Yang Y., Gu Z., Zhou Z. (2013). Comparison of adhesive gut bacteria composition, immunity, and disease resistance in juvenile hybrid tilapia fed two different Lactobacillus strains. Fish and Shellfish Immunology, 35: 54-62.

Merrifield D.L., Carnevali O. (2014). Probiotic modulation of the gut microbiota of fish. Aquaculture Nutrition: Gut Health, Probiotics and Prebiotics, 185-222.

Paray B.A., Hoseini S.M., Hoseinifar S.H., Van Doan H. (2020). Effects of dietary oak (Quercus castaneifolia) leaf extract on growth, antioxidant, and immune characteristics and responses to crowding stress in common carp (Cyprinus carpio). Aquaculture (Amsterdam, Netherlands), 735276.

Piazzon M.C., Calduch-Giner J.A., Fouz B., Estensoro I., Simó-Mirabet P., Puyalto M., Karalazos V., Palenzuela O., Sitjí -Bobadilla A., Pérez-Sánchez J. (2017). Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets. Microbiome, 5: 164.

Pimentel S.S., Katagiri T. (2008). Differences of probiotic effects on Edwardsiella tarda challenged Nile tilapia (Oreochromis niloticus) fed with four Lactobacillus species. Aquaculture Science, 56: 401-408.

Ringo E., Hossein S., Ghosh K., Doan H.V., Beck B.R., Song S. (2018). Lactic acid bacteria in finfish–an update. Frontiers in Microbiology, 9: 1818.

Taheri Mirghaed A., Fayaz S., Hoseini S.M. (2019a). Effects of dietary 1, 8-cineole supplementation on serum stress and antioxidant markers of common carp (Cyprinus carpio) acutely exposed to ambient ammonia. Aquaculture (Amsterdam, Netherlands), 509: 8-15.

Taheri Mirghaed A., Yarahmadi P., Soltani M., Paknejad H., Hoseini S.M. (2019b). Dietary sodium butyrate (Butirex® C4) supplementation modulates intestinal transcriptomic responses and augments disease resistance of rainbow trout (Oncorhynchus mykiss). Fish and Shellfish Immunology, 92: 621-628.

Taheri Mirghaed A., Yarahmadi P., Hosseinifar S.H., Tahmasebi D., Gheisvandi N., Ghaedi A. (2018). The effects singular or combined administration of fermentable fiber and probiotic on mucosal immune parameters, digestive enzyme activity, gut microbiota and growth performance of Caspian white fish (Rutilus frisii kutum) fingerlings. Fish and Shellfish Immunology, 77: 194-199.

Tarkhani R., Imani A., Hoseinifar S.H., Moghanlou K.S., Manaffar R. (2020). The effects of host-associated Enterococcus faecium CGMCC1. 2136 on serum immune parameters, digestive enzymes activity and growth performance of the Caspian roach (Rutilus rutilus caspicus) fingerlings. Aquaculture (Amsterdam, Netherlands), 519: 734741.

Yousefi M., Vatnikov Y.A., Kulikov E.V., Plushikov V.G., Drukovsky S.G., Hoseinifar S.H., Van Doan H. (2020). The protective effects of dietary garlic on common carp (Cyprinus carpio) exposed to ambient ammonia toxicity. Aquaculture (Amsterdam, Netherlands), 735400.

Zargar A., Taheri Mirghaed A., Mirzargar S.S., Ghelichpour M., Yousefi M., Hoseini S.M. (2020). Dietary ginger administration attenuates oxidative stress and immunosuppression caused by oxytetracycline in rainbow trout (Oncorhynchus mykiss). Aquaculture Research: In press.

Zhou Z., Wang W., Liu W., Gatlin III D.M., Zhang Y., Yao B., Ringí¸ E. (2012). Identification of highly-adhesive gut Lactobacillus strains in zebrafish (Danio rerio) by partial rpoB gene sequence analysis. Aquaculture (Amsterdam, Netherlands), 370: 150-157.